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Registro Completo |
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Biblioteca(s): |
Biblioteca Rui Tendinha. |
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Data corrente: |
02/07/2019 |
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Data da última atualização: |
02/07/2019 |
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Tipo da produção científica: |
Publicação em Anais de Congresso |
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Autoria: |
OLIVEIRA, C. M. de; RODRIGUES, W. N.; FERRÃO, M. A. G.; FERRÃO, R. G.; FONSECA, A. F. A. da.; TOMAZ, M. A. |
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Afiliação: |
Cíntia Machado de Oliveira, UENF; Wagner Nunes Rodrigues, UFES; Maria Amélia Gava Ferrão, Incaper/Embrapa Café; Romário Gava Ferrão, Incaper; Aymbiré Francisco Almeida da Fonseca, Incaper/Embrapa Café; Marcelo Antonio Tomaz, UFES. |
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Título: |
Severidade do bicho mineiro em genótipos de café conilon de ciclo tardio selecionados no Norte e no Sul do Estado do Espírito Santo. |
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Ano de publicação: |
2011 |
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Fonte/Imprenta: |
In: SIMPÓSIO DE PESQUISA DOS CAFÉS DO BRASIL, 7., 2011, Araxá. Anais... Brasília, DF: Embrapa Café, 2011 |
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Idioma: |
Português |
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Conteúdo: |
Este trabalho foi realizado na Fazenda Experimental de Bananal do Norte (Incaper) no sul do Estado, em delineamento em blocos casualizados, com quatro repetições e cinco plantas por parcela. Foram avaliados 40 genótipos de maturação tardia, que passaram por um contínuo processo de seleção em duas regiões diferentes, no norte e no sul do Estado. Objetivou-se estudar a severidade do bicho mineiro em genótipos de café conilon de ciclo de maturação tardio, selecionados no norte e no sul do Estado do Espírito Santo. Os 40 genótipos de café conilon de ciclo de maturação tardio apresentam diversidade quanto à severidade do bicho-mineiro. Os genótipos selecionados tanto no norte quanto no sul demonstraram comportamento semelhante, concentraram-se nas classes de menor severidade e de severidade intermediárias. |
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Palavras-Chave: |
Genótipos. |
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Thesagro: |
Coffea Canephora; Leucoptera Coffeella; Praga. |
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Categoria do assunto: |
G Melhoramento Genético |
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URL: |
https://biblioteca.incaper.es.gov.br/digital/bitstream/123456789/3618/1/Severidade-do-bicho-mineiro.pdf
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Marc: |
LEADER 01592nam a2200217 a 4500
001 1021458
005 2019-07-02
008 2011 bl uuuu u01u1 u #d
100 1 $aOLIVEIRA, C. M. de
245 $aSeveridade do bicho mineiro em genótipos de café conilon de ciclo tardio selecionados no Norte e no Sul do Estado do Espírito Santo.$h[electronic resource]
260 $aIn: SIMPÓSIO DE PESQUISA DOS CAFÉS DO BRASIL, 7., 2011, Araxá. Anais... Brasília, DF: Embrapa Café$c2011
520 $aEste trabalho foi realizado na Fazenda Experimental de Bananal do Norte (Incaper) no sul do Estado, em delineamento em blocos casualizados, com quatro repetições e cinco plantas por parcela. Foram avaliados 40 genótipos de maturação tardia, que passaram por um contínuo processo de seleção em duas regiões diferentes, no norte e no sul do Estado. Objetivou-se estudar a severidade do bicho mineiro em genótipos de café conilon de ciclo de maturação tardio, selecionados no norte e no sul do Estado do Espírito Santo. Os 40 genótipos de café conilon de ciclo de maturação tardio apresentam diversidade quanto à severidade do bicho-mineiro. Os genótipos selecionados tanto no norte quanto no sul demonstraram comportamento semelhante, concentraram-se nas classes de menor severidade e de severidade intermediárias.
650 $aCoffea Canephora
650 $aLeucoptera Coffeella
650 $aPraga
653 $aGenótipos
700 1 $aRODRIGUES, W. N.
700 1 $aFERRÃO, M. A. G.
700 1 $aFERRÃO, R. G.
700 1 $aFONSECA, A. F. A. da.
700 1 $aTOMAZ, M. A.
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Registro original: |
Biblioteca Rui Tendinha (BRT) |
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Biblioteca |
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Tipo/Formato |
Classificação |
Cutter |
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Registro Completo |
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Biblioteca(s): |
Biblioteca Rui Tendinha. |
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Data corrente: |
09/09/2020 |
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Data da última atualização: |
09/09/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
DUARTE, M. F.; PEREIRA-CARVALHO, R. de C.; REIS, L. de N. A. dos; ROJAS, M. J.; GILBERTSON, R.; COSTA, H.; BOITEUX, L. S.; FONSECA, M. E. N. |
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Afiliação: |
Macaria Ferreira Duarte, UnB; Rita de Cássia Pereira Carvalho, UnB; Luciane de Nazaré Almeida dos Reis, UnB; Maria J. Rojas, UC-DAVIS; Robert Gilbertson, UC-DAVIS; Helcio Costa, Incaper; Leonardo Silva Boiteux, EMBRAPA; Maria Esther N. Fonseca, EMBRAPA. |
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Título: |
Natural Infection of Tomatoes (Solanum lycopersicum) by Euphorbia yellow mosaic virus Isolates across Four Brazilian States. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Plant Disease, set. 2020. |
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Idioma: |
Inglês |
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Conteúdo: |
Severe yield losses induced by a complex of whitefly?transmitted Begomovirus species (family Geminiviridae) have been reported in tomatoes in Brazil (Reis et al. 2020). Nine isolates were obtained from tomato plants exhibiting begomovirus?like symptoms (viz. apical and interveinal chlorosis, yellow spots, and stunting) during independent field surveys: one isolate in Sumaré, São Paulo?SP State (isolate SP?066) in 2001, two in Serra Negra, Minas Gerais?MG (MG?012 and MG?016) in 2002, five in Caxias do Sul, Rio Grande do Sul?RS (RS?039, RS?045, RS?046, RS?047 and RS?058) in 2011 and one in Domingos Martins, Espírito Santo?ES (ES?148) in 2016. Disease incidence across all sampled fields ranged from 30% (in Domingos Martins?ES) to 90% in Sumaré?SP. Total DNA extraction was done by a modified CTAB method (Boiteux et al., 1999). Begomovirus infection was confirmed in all isolates by selective amplification of viral DNA?A segments using the primer pairs ?PAL1v1978 / PAR1c496? (Rojas et al., 1993) and ?BegomoAFor1? / ?BegomoARev1? (Ha et al., 2006), which produce two large and non?overlapping segments (≈1120 bp and ≈1205 bp, respectively). These PCR amplicons were initially characterized via direct Sanger dideoxy sequencing at CNPH. BLASTn analysis of the partial DNA?A genomes of these nine isolates indicated identity levels of 95?97% to three euphorbia yellow mosaic virus (EuYMV) reference isolates (= KY559532, JF756674, and KY559583) found infecting the weed Euphorbia heterophylla L. The entire DNA?A (2,609 nts = MN746971) and DNA?B (2,579 nts = MN746970) components of the MG?016 isolate were obtained via high?performance sequencing using Illumina HiSeq 2500 system (Macrogen Inc., South Korea). Sequences were assembled with the CLC Genomics Workbench program 10. Contigs were validated by BLASTx and BLASTn and compared to the ssDNA virus database at NCBI (www.ncbi.nlm.nih.gov). The fully?characterized MG?016 isolate displayed identity levels ranging from 97 to 99% to the EuYMV reference isolates as well as similar genomic features such as the conserved TATA box, nonanucleotide, and iterons (that were in agreement with a cognate nature of the DNA?A and DNA?B components). A partial sequence of the DNA?B genome was also obtained for the MG?012 isolate (MT7831942). The isolates MG?012 and MG?016 were found in mixed infections with tomato severe rugose virus (ToSRV) and tomato golden vein virus (TGVV), respectively. In addition, the complete DNA?A genomes of ES?148 (MN746972) and SP?066 (MN782438) were also obtained via a combination of primer walking and Sanger dideoxy sequencing, displaying 96?98% identity to EuYMV isolates. To our knowledge, this is the first report of multiple and independent events of natural infection of tomatoes by EuYMV isolates. Our results confirm the natural host status of tomatoes to EuYMV isolates as indicated in previous infectivity assays using biolistic inoculation (Barreto et al., 2013). The weed E. heterophylla is widely disseminated and very often present within tomato fields due to its higher levels of tolerance to the major herbicide (metribuzin) employed in this crop. Therefore, this weed may act as a persistent reservoir of tomato?infecting EuYMV isolates, which may allow the selection of viral populations potentially more adapted to this vegetable crop. MenosSevere yield losses induced by a complex of whitefly?transmitted Begomovirus species (family Geminiviridae) have been reported in tomatoes in Brazil (Reis et al. 2020). Nine isolates were obtained from tomato plants exhibiting begomovirus?like symptoms (viz. apical and interveinal chlorosis, yellow spots, and stunting) during independent field surveys: one isolate in Sumaré, São Paulo?SP State (isolate SP?066) in 2001, two in Serra Negra, Minas Gerais?MG (MG?012 and MG?016) in 2002, five in Caxias do Sul, Rio Grande do Sul?RS (RS?039, RS?045, RS?046, RS?047 and RS?058) in 2011 and one in Domingos Martins, Espírito Santo?ES (ES?148) in 2016. Disease incidence across all sampled fields ranged from 30% (in Domingos Martins?ES) to 90% in Sumaré?SP. Total DNA extraction was done by a modified CTAB method (Boiteux et al., 1999). Begomovirus infection was confirmed in all isolates by selective amplification of viral DNA?A segments using the primer pairs ?PAL1v1978 / PAR1c496? (Rojas et al., 1993) and ?BegomoAFor1? / ?BegomoARev1? (Ha et al., 2006), which produce two large and non?overlapping segments (≈1120 bp and ≈1205 bp, respectively). These PCR amplicons were initially characterized via direct Sanger dideoxy sequencing at CNPH. BLASTn analysis of the partial DNA?A genomes of these nine isolates indicated identity levels of 95?97% to three euphorbia yellow mosaic virus (EuYMV) reference isolates (= KY559532, JF756674, and KY559583) found infecting the weed Euphorbia ... Mostrar Tudo |
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Thesagro: |
Doença de Planta; Erva Daninha; Mosaico; Tomate. |
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Thesaurus NAL: |
Begomovirus. |
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Categoria do assunto: |
H Saúde e Patologia |
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URL: |
https://biblioteca.incaper.es.gov.br/digital/bitstream/123456789/4073/1/natural-infection-tomatoes-costa.pdf
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Marc: |
LEADER 04125naa a2200265 a 4500
001 1022277
005 2020-09-09
008 2020 bl uuuu u00u1 u #d
100 1 $aDUARTE, M. F.
245 $aNatural Infection of Tomatoes (Solanum lycopersicum) by Euphorbia yellow mosaic virus Isolates across Four Brazilian States.$h[electronic resource]
260 $c2020
520 $aSevere yield losses induced by a complex of whitefly?transmitted Begomovirus species (family Geminiviridae) have been reported in tomatoes in Brazil (Reis et al. 2020). Nine isolates were obtained from tomato plants exhibiting begomovirus?like symptoms (viz. apical and interveinal chlorosis, yellow spots, and stunting) during independent field surveys: one isolate in Sumaré, São Paulo?SP State (isolate SP?066) in 2001, two in Serra Negra, Minas Gerais?MG (MG?012 and MG?016) in 2002, five in Caxias do Sul, Rio Grande do Sul?RS (RS?039, RS?045, RS?046, RS?047 and RS?058) in 2011 and one in Domingos Martins, Espírito Santo?ES (ES?148) in 2016. Disease incidence across all sampled fields ranged from 30% (in Domingos Martins?ES) to 90% in Sumaré?SP. Total DNA extraction was done by a modified CTAB method (Boiteux et al., 1999). Begomovirus infection was confirmed in all isolates by selective amplification of viral DNA?A segments using the primer pairs ?PAL1v1978 / PAR1c496? (Rojas et al., 1993) and ?BegomoAFor1? / ?BegomoARev1? (Ha et al., 2006), which produce two large and non?overlapping segments (≈1120 bp and ≈1205 bp, respectively). These PCR amplicons were initially characterized via direct Sanger dideoxy sequencing at CNPH. BLASTn analysis of the partial DNA?A genomes of these nine isolates indicated identity levels of 95?97% to three euphorbia yellow mosaic virus (EuYMV) reference isolates (= KY559532, JF756674, and KY559583) found infecting the weed Euphorbia heterophylla L. The entire DNA?A (2,609 nts = MN746971) and DNA?B (2,579 nts = MN746970) components of the MG?016 isolate were obtained via high?performance sequencing using Illumina HiSeq 2500 system (Macrogen Inc., South Korea). Sequences were assembled with the CLC Genomics Workbench program 10. Contigs were validated by BLASTx and BLASTn and compared to the ssDNA virus database at NCBI (www.ncbi.nlm.nih.gov). The fully?characterized MG?016 isolate displayed identity levels ranging from 97 to 99% to the EuYMV reference isolates as well as similar genomic features such as the conserved TATA box, nonanucleotide, and iterons (that were in agreement with a cognate nature of the DNA?A and DNA?B components). A partial sequence of the DNA?B genome was also obtained for the MG?012 isolate (MT7831942). The isolates MG?012 and MG?016 were found in mixed infections with tomato severe rugose virus (ToSRV) and tomato golden vein virus (TGVV), respectively. In addition, the complete DNA?A genomes of ES?148 (MN746972) and SP?066 (MN782438) were also obtained via a combination of primer walking and Sanger dideoxy sequencing, displaying 96?98% identity to EuYMV isolates. To our knowledge, this is the first report of multiple and independent events of natural infection of tomatoes by EuYMV isolates. Our results confirm the natural host status of tomatoes to EuYMV isolates as indicated in previous infectivity assays using biolistic inoculation (Barreto et al., 2013). The weed E. heterophylla is widely disseminated and very often present within tomato fields due to its higher levels of tolerance to the major herbicide (metribuzin) employed in this crop. Therefore, this weed may act as a persistent reservoir of tomato?infecting EuYMV isolates, which may allow the selection of viral populations potentially more adapted to this vegetable crop.
650 $aBegomovirus
650 $aDoença de Planta
650 $aErva Daninha
650 $aMosaico
650 $aTomate
700 1 $aPEREIRA-CARVALHO, R. de C.
700 1 $aREIS, L. de N. A. dos
700 1 $aROJAS, M. J.
700 1 $aGILBERTSON, R.
700 1 $aCOSTA, H.
700 1 $aBOITEUX, L. S.
700 1 $aFONSECA, M. E. N.
773 $tPlant Disease, set. 2020.
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