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Registro Completo |
Biblioteca(s): |
Biblioteca Rui Tendinha. |
Data corrente: |
19/12/2022 |
Data da última atualização: |
19/12/2022 |
Autoria: |
MORENO, P. V.; ZULATO, J. B.; SOUZA, N. A. de |
Afiliação: |
Pedro Vale Moreno; Jair Barbosa Zulato; Noely Antonio de Souza. |
Título: |
Calendário mensal de trabalho: Uma orientação. |
Ano de publicação: |
1985 |
Fonte/Imprenta: |
Brasília: EMBRATER, 1985. |
Páginas: |
11 p. |
Série: |
(Serie Leituras Selecionadas-20). |
Idioma: |
Português |
Conteúdo: |
Um instrumento de planejamento a ser utilizado a nível local pelos extensionistas para, a partir do Cronograma de Atividades do Programa Local, detalhar e reprogramar as ações a serem desenvolvidas no mês vigente. |
Thesagro: |
Extensão Rural; Extensionista; Método de Pesquisa; Pesquisa; Planejamento; Programa de Pesquisa. |
Categoria do assunto: |
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Marc: |
LEADER 00817nam a2200229 a 4500 001 1024603 005 2022-12-19 008 1985 bl uuuu u0uu1 u #d 100 1 $aMORENO, P. V. 245 $aCalendário mensal de trabalho$bUma orientação. 260 $aBrasília: EMBRATER$c1985 300 $a11 p. 490 $a(Serie Leituras Selecionadas-20). 520 $aUm instrumento de planejamento a ser utilizado a nível local pelos extensionistas para, a partir do Cronograma de Atividades do Programa Local, detalhar e reprogramar as ações a serem desenvolvidas no mês vigente. 650 $aExtensão Rural 650 $aExtensionista 650 $aMétodo de Pesquisa 650 $aPesquisa 650 $aPlanejamento 650 $aPrograma de Pesquisa 700 1 $aZULATO, J. B. 700 1 $aSOUZA, N. A. de
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Biblioteca Rui Tendinha (BRT) |
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Registro Completo |
Biblioteca(s): |
Biblioteca Rui Tendinha. |
Data corrente: |
30/12/2014 |
Data da última atualização: |
01/09/2015 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 1 |
Autoria: |
COSTA, H. B.; FERNANDES, P. M. B.; ROMÃO, W.; VENTURA, J. A. |
Afiliação: |
Helber B. Costa; Patricia M.B. Fernandes; Wanderson Romão; Jose Aires Ventura, Incaper. |
Título: |
A new procedure based on column chromatography to purify bromelain by ion exchange plus gel filtration chromatographies. |
Ano de publicação: |
2014 |
Fonte/Imprenta: |
Industrial Crops and Products, Vol. 59, p. 163-168, august 2014. |
Páginas: |
6 p. |
Idioma: |
Inglês |
Conteúdo: |
Bioproducts separation and purification processes are an important segment of the biotechnical industry. Bromelain is an enzyme which has great commercial value and is of wide interest in the pharmaceutical, alimentary, and textile industries, among others. The goal of this study was to develop a new method for bromelain purification from the stem residues resulting from agricultural processing of the pineapple plant. Bromelain was purified using two liquid chromatography steps, ion exchange plus gel filtration chromatography. Use of the methodology which was developed produced an enzyme with a molecular weight of 30 kDa (confirmed by SDS-PAGE), high recovery of enzymatic activity (89%), and with a purification factor of 16.93, a result superior to the methodologies described in the literature. HPLC showed the presence of two peaks in the ion exchange chromatogram and only one protein in the gel filtration chromatogram. Results indicate that, depending on the destination of the bromelain, the process can be stopped after the first purification step. The MALDI-TOF MS provided the peptide mass fingerprint of bromelain and MALDI-MS/MS the fragmentation profile and sequencing of the ions of m/z 951 and 1584. Thus, the connectivity and chemical structure of bromelain was confirmed. Moreover, besides its superiority to other methodologies, it can be applied to take advantage of the agricultural and industrial pineapple plant residues. |
Thesaurus NAL: |
Bromelain; Enzyme; Forbromelain; Pineapple; Purification. |
Categoria do assunto: |
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URL: |
http://biblioteca.incaper.es.gov.br/digital/bitstream/item/419/1/New-Procedure-Chromatography-to-Purify-Bromelain-2014.pdf
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Marc: |
LEADER 02130naa a2200229 a 4500 001 1004754 005 2015-09-01 008 2014 bl uuuu u00u1 u #d 100 1 $aCOSTA, H. B. 245 $aA new procedure based on column chromatography to purify bromelain by ion exchange plus gel filtration chromatographies.$h[electronic resource] 260 $c2014 300 $a6 p. 520 $aBioproducts separation and purification processes are an important segment of the biotechnical industry. Bromelain is an enzyme which has great commercial value and is of wide interest in the pharmaceutical, alimentary, and textile industries, among others. The goal of this study was to develop a new method for bromelain purification from the stem residues resulting from agricultural processing of the pineapple plant. Bromelain was purified using two liquid chromatography steps, ion exchange plus gel filtration chromatography. Use of the methodology which was developed produced an enzyme with a molecular weight of 30 kDa (confirmed by SDS-PAGE), high recovery of enzymatic activity (89%), and with a purification factor of 16.93, a result superior to the methodologies described in the literature. HPLC showed the presence of two peaks in the ion exchange chromatogram and only one protein in the gel filtration chromatogram. Results indicate that, depending on the destination of the bromelain, the process can be stopped after the first purification step. The MALDI-TOF MS provided the peptide mass fingerprint of bromelain and MALDI-MS/MS the fragmentation profile and sequencing of the ions of m/z 951 and 1584. Thus, the connectivity and chemical structure of bromelain was confirmed. Moreover, besides its superiority to other methodologies, it can be applied to take advantage of the agricultural and industrial pineapple plant residues. 650 $aBromelain 650 $aEnzyme 650 $aForbromelain 650 $aPineapple 650 $aPurification 700 1 $aFERNANDES, P. M. B. 700 1 $aROMÃO, W. 700 1 $aVENTURA, J. A. 773 $tIndustrial Crops and Products, Vol. 59, p. 163-168, august 2014.
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