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Registros recuperados : 4 | |
Registros recuperados : 4 | |
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Registro Completo |
Biblioteca(s): |
Biblioteca Rui Tendinha. |
Data corrente: |
04/08/2016 |
Data da última atualização: |
04/08/2016 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
- - - |
Autoria: |
ATHAYDE, J. T.; ROMEIRO, R. da S. |
Afiliação: |
José Tadeu Athayde, EMCAPA; Reginaldo da Silva Romeiro, UFV. |
Título: |
Atividade biológica do exopolissacarídeos de Xantomonas campestris pv. manihotis. |
Ano de publicação: |
1983 |
Fonte/Imprenta: |
FITOPOPATOLOGIA BRASILEIRA, v.8 n.3 p. 485-90, |
Idioma: |
Português |
Conteúdo: |
A crude preparation of the exopolysaccaride (EPS) of Xanthomonas campestris pv. manihotis (Berthet & Bondar, 1915) Dye 1978 was obtained by growing the pathogen on solid culture medium for 30 h at 280C. The bacterial growth was harvested in PBS and the
suspension contrefuged at 10.000 g/20 mino Ethanol was added to the supernatant to a concentraction of 70% giving rise to a white precipitate that was dialysed against destilled water. The dialysate was considered the crude preparation of the EPS. Cassava shoots ('Chagas' and 'Branquinha' cultuvars) were used to test biological acitivity of the cru de EPS. The basal part of shoots was immersed in different dilutions of the cru de EPS in destilled water. The time for wilting was inversely proportional to EPS concentration. Turgescence recorver was observed when 0,5 em of the basal part of the shoots was remved ant the shoots transfered to destilled water. |
Palavras-Chave: |
Patógeno; Polissacarídeos; Xantomonas campestris. |
Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
URL: |
http://biblioteca.incaper.es.gov.br/digital/bitstream/item/2073/1/atividade-biologica-do-polissacarideo0001.pdf
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Marc: |
LEADER 01422naa a2200169 a 4500 001 1011435 005 2016-08-04 008 1983 bl uuuu u00u1 u #d 100 1 $aATHAYDE, J. T. 245 $aAtividade biológica do exopolissacarídeos de Xantomonas campestris pv. manihotis.$h[electronic resource] 260 $c1983 520 $aA crude preparation of the exopolysaccaride (EPS) of Xanthomonas campestris pv. manihotis (Berthet & Bondar, 1915) Dye 1978 was obtained by growing the pathogen on solid culture medium for 30 h at 280C. The bacterial growth was harvested in PBS and the suspension contrefuged at 10.000 g/20 mino Ethanol was added to the supernatant to a concentraction of 70% giving rise to a white precipitate that was dialysed against destilled water. The dialysate was considered the crude preparation of the EPS. Cassava shoots ('Chagas' and 'Branquinha' cultuvars) were used to test biological acitivity of the cru de EPS. The basal part of shoots was immersed in different dilutions of the cru de EPS in destilled water. The time for wilting was inversely proportional to EPS concentration. Turgescence recorver was observed when 0,5 em of the basal part of the shoots was remved ant the shoots transfered to destilled water. 653 $aPatógeno 653 $aPolissacarídeos 653 $aXantomonas campestris 700 1 $aROMEIRO, R. da S. 773 $tFITOPOPATOLOGIA BRASILEIRA$gv.8 n.3 p. 485-90
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