| |
|
|
 | Acesso ao texto completo restrito à biblioteca da Biblioteca Rui Tendinha. Para informações adicionais entre em contato com biblioteca@incaper.es.gov.br. |
|
Registro Completo |
|
Biblioteca(s): |
Biblioteca Rui Tendinha. |
|
Data corrente: |
13/01/2015 |
|
Data da última atualização: |
13/01/2015 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Autoria: |
MARRACCINI, P.; VINECKY, F.; ALVES, G. S. C.; RAMOS, H. J. O.; ELBELT, S.; VIEIRA, N. G.; CARNEIRO, F. A.; SUJII, P. S.; ALEKCEVETCH, J. C.; SILVA, V. A.; DaMATTA, F. M.; FERRÃO, M. A. G.; LEROY, T.; POT, D.; VIEIRA, L. G. E.; SILVA, F. R. da; ANDRADE, A. C. |
|
Afiliação: |
Maria Amélia Gava Ferrão, Incaper/Embrapa Café. |
|
Título: |
Differentially expressed genes and proteins upon drought acclimation in tolerant and sensitive genotypes of Coffea canephora. |
|
Ano de publicação: |
2012 |
|
Fonte/Imprenta: |
Journal of Experimental Botany, Oxford v. 63, n. 11, p. 4191-4212, 2012. |
|
Idioma: |
Inglês |
|
Conteúdo: |
The aim of this study was to investigate the molecular mechanisms underlying drought acclimation in coffee plants by the identification of candidate genes (CGs) using different approaches. The first approach used the data generated during the Brazilian Coffee expressed sequence tag (EST) project to select 13 CGs by an in silico analysis (electronic northern). The second approach was based on screening macroarrays spotted with plasmid DNA (coffee ESTs) with separate hybridizations using leaf cDNA probes from drought-tolerant and susceptible clones of Coffea canephora var. Conilon, grown under different water regimes. This allowed the isolation of seven additional CGs. The third approach used two-dimensional gel electrophoresis to identify proteins displaying differential accumulation in leaves of drought-tolerant and susceptible clones of C. canephora. Six of them were characterized by MALDI-TOF-MS/MS (matrix-assisted laser desorption-time of flight-tandem mass spectrometry) and the corresponding proteins were identified. Finally, additional CGs were selected from the literature, and quantitative real-time polymerase chain reaction (qPCR) was performed to analyse the expression of all identified CGs. Altogether, >40 genes presenting differential gene expression during drought acclimation were identified, some of them showing different expression profiles between drought-tolerant and susceptible clones. Based on the obtained results, it can be concluded that factors involved a complex network of responses probably involving the abscisic signalling pathway and nitric oxide are major molecular determinants that might explain the better efficiency in controlling stomata closure and transpiration displayed by drought-tolerant clones of C. canephora. MenosThe aim of this study was to investigate the molecular mechanisms underlying drought acclimation in coffee plants by the identification of candidate genes (CGs) using different approaches. The first approach used the data generated during the Brazilian Coffee expressed sequence tag (EST) project to select 13 CGs by an in silico analysis (electronic northern). The second approach was based on screening macroarrays spotted with plasmid DNA (coffee ESTs) with separate hybridizations using leaf cDNA probes from drought-tolerant and susceptible clones of Coffea canephora var. Conilon, grown under different water regimes. This allowed the isolation of seven additional CGs. The third approach used two-dimensional gel electrophoresis to identify proteins displaying differential accumulation in leaves of drought-tolerant and susceptible clones of C. canephora. Six of them were characterized by MALDI-TOF-MS/MS (matrix-assisted laser desorption-time of flight-tandem mass spectrometry) and the corresponding proteins were identified. Finally, additional CGs were selected from the literature, and quantitative real-time polymerase chain reaction (qPCR) was performed to analyse the expression of all identified CGs. Altogether, >40 genes presenting differential gene expression during drought acclimation were identified, some of them showing different expression profiles between drought-toler... Mostrar Tudo |
|
Palavras-Chave: |
Candidate gene; Coffea canephora; Differential expression; Drought acclimation; Genética; Proteomic; Proteômica; Real time PCR. |
|
Thesagro: |
Café; Coffea canephora; Genetics. |
|
Categoria do assunto: |
-- |
|
Marc: |
LEADER 03238naa a2200445 a 4500
001 1004907
005 2015-01-13
008 2012 bl uuuu u00u1 u #d
100 1 $aMARRACCINI, P.
245 $aDifferentially expressed genes and proteins upon drought acclimation in tolerant and sensitive genotypes of Coffea canephora.$h[electronic resource]
260 $c2012
520 $aThe aim of this study was to investigate the molecular mechanisms underlying drought acclimation in coffee plants by the identification of candidate genes (CGs) using different approaches. The first approach used the data generated during the Brazilian Coffee expressed sequence tag (EST) project to select 13 CGs by an in silico analysis (electronic northern). The second approach was based on screening macroarrays spotted with plasmid DNA (coffee ESTs) with separate hybridizations using leaf cDNA probes from drought-tolerant and susceptible clones of Coffea canephora var. Conilon, grown under different water regimes. This allowed the isolation of seven additional CGs. The third approach used two-dimensional gel electrophoresis to identify proteins displaying differential accumulation in leaves of drought-tolerant and susceptible clones of C. canephora. Six of them were characterized by MALDI-TOF-MS/MS (matrix-assisted laser desorption-time of flight-tandem mass spectrometry) and the corresponding proteins were identified. Finally, additional CGs were selected from the literature, and quantitative real-time polymerase chain reaction (qPCR) was performed to analyse the expression of all identified CGs. Altogether, >40 genes presenting differential gene expression during drought acclimation were identified, some of them showing different expression profiles between drought-tolerant and susceptible clones. Based on the obtained results, it can be concluded that factors involved a complex network of responses probably involving the abscisic signalling pathway and nitric oxide are major molecular determinants that might explain the better efficiency in controlling stomata closure and transpiration displayed by drought-tolerant clones of C. canephora.
650 $aCafé
650 $aCoffea canephora
650 $aGenetics
653 $aCandidate gene
653 $aCoffea canephora
653 $aDifferential expression
653 $aDrought acclimation
653 $aGenética
653 $aProteomic
653 $aProteômica
653 $aReal time PCR
700 1 $aVINECKY, F.
700 1 $aALVES, G. S. C.
700 1 $aRAMOS, H. J. O.
700 1 $aELBELT, S.
700 1 $aVIEIRA, N. G.
700 1 $aCARNEIRO, F. A.
700 1 $aSUJII, P. S.
700 1 $aALEKCEVETCH, J. C.
700 1 $aSILVA, V. A.
700 1 $aDaMATTA, F. M.
700 1 $aFERRÃO, M. A. G.
700 1 $aLEROY, T.
700 1 $aPOT, D.
700 1 $aVIEIRA, L. G. E.
700 1 $aSILVA, F. R. da
700 1 $aANDRADE, A. C.
773 $tJournal of Experimental Botany, Oxford$gv. 63, n. 11, p. 4191-4212, 2012.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Biblioteca Rui Tendinha (BRT) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
|
|
|
Registro Completo |
|
Biblioteca(s): |
Biblioteca Rui Tendinha. |
|
Data corrente: |
04/02/2020 |
|
Data da última atualização: |
14/12/2020 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Circulação/Nível: |
B - 1 |
|
Autoria: |
MAURI, A. L.; ARAÚJO, E. F.; AMARO, H. T. R.; ARAUJO, R. F.; POSSE, S. C. P. |
|
Afiliação: |
Aldo Luiz Mauri, Incaper; Eduardo Fontes Araujo, UFV; Hugo Tiago Ribeiro Amaro, Unimontes; Roberto Fontes Araujo, EPAMIG; Sheila Cristina Prucoli Posse, Incaper. |
|
Título: |
Tratamentos sanitários na qualidade fisiológica e sanitária de sementes de tomate produzidas sob manejo orgânico. |
|
Ano de publicação: |
2019 |
|
Fonte/Imprenta: |
Revista de Ciências Agrárias, v. 452, n. 4, p. 991-999, 2019. |
|
DOI: |
https://doi.org/10.19084/rca.17142 |
|
Idioma: |
Português |
|
Conteúdo: |
O uso de sementes de alta qualidade fisiológica e sanitária representa importante estratégia na implantação de lavouras de tomate orgânico. Este trabalho foi realizado com o objetivo de avaliar o efeito de tratamentos sanitários na qualidade fisiológica e sanitária de sementes de tomate cereja produzidas sob manejo orgânico. Foram utilizados seis lotes de sementes de tomate cereja oriundos do Rio Grande do Sul e do Espírito Santo. As sementes foram submetidas a tratamentos sanitários alternativos que constaram de imersão das sementes em extratos vegetais (alfavaca-cravo e timbó) e em água destilada, microbiolização das sementes, termoterapia, além de um tratamento padrão com fungicida comercial e uma testemunha sem tratamento. Em seguida, as sementes foram avaliadas quanto à qualidade fisiológica e sanitária. O extrato de timbó reduziu a qualidade fisiológica das sementes de tomate. O tratamento de sementes com extrato de alfavaca-cravo e extrato de timbó inibiu a incidência de Penicillium sp. em sementes de tomate cereja, produzidas sob manejo orgânico. O extrato de alfavaca-cravo é eficiente na manutenção da qualidade fisiológica e sanitária das sementes de tomate cereja sob manejo orgânico.
The use of high quality physiological and sanitary seeds represents an important strategy in the implantation of organic tomato crops. This work was carried out to evaluate the effect of sanitary treatments on physiological and sanitary quality of seeds of tomato grown under organic management. We used six seed lots of cherry tomatoes coming from Rio Grande do Sul and Espírito Santo. The seeds were subjected to alternative sanitary treatments that consisted of soaking seeds in plant extracts and in distilled water, seeds microbiolization, thermotherapy, in addition to standard treatment with commercial fungicide and a control without any treatment. Then the seeds were evaluated for physiological and sanitary quality. Timbó extract reduced the physiological quality of tomato seeds. The treatment of seeds with alfavaca-clove extract and timbó extract inhibited the incidence of Penicillium sp. in cherry tomato seeds, produced under organic management. The alfavaca-cravo extract is efficient in maintaining the physiological and sanitary quality of cherry tomato seeds under organic management. MenosO uso de sementes de alta qualidade fisiológica e sanitária representa importante estratégia na implantação de lavouras de tomate orgânico. Este trabalho foi realizado com o objetivo de avaliar o efeito de tratamentos sanitários na qualidade fisiológica e sanitária de sementes de tomate cereja produzidas sob manejo orgânico. Foram utilizados seis lotes de sementes de tomate cereja oriundos do Rio Grande do Sul e do Espírito Santo. As sementes foram submetidas a tratamentos sanitários alternativos que constaram de imersão das sementes em extratos vegetais (alfavaca-cravo e timbó) e em água destilada, microbiolização das sementes, termoterapia, além de um tratamento padrão com fungicida comercial e uma testemunha sem tratamento. Em seguida, as sementes foram avaliadas quanto à qualidade fisiológica e sanitária. O extrato de timbó reduziu a qualidade fisiológica das sementes de tomate. O tratamento de sementes com extrato de alfavaca-cravo e extrato de timbó inibiu a incidência de Penicillium sp. em sementes de tomate cereja, produzidas sob manejo orgânico. O extrato de alfavaca-cravo é eficiente na manutenção da qualidade fisiológica e sanitária das sementes de tomate cereja sob manejo orgânico.
The use of high quality physiological and sanitary seeds represents an important strategy in the implantation of organic tomato crops. This work was carried out to evaluate the effect of sanitary treatments on physiological and sanitary quality of seeds of tomato grown under organic m... Mostrar Tudo |
|
Palavras-Chave: |
Agricultura orgânica; Germinação; Lycopersicon esculentum Mill; Produção de sementes; Tomate; Tomate orgânico. |
|
Thesagro: |
Agricultura Orgânica; Semente; Teste de Sementes; Tomate. |
|
Thesaurus NAL: |
Germination; Organic farming; Seed production. |
|
Categoria do assunto: |
H Saúde e Patologia |
|
URL: |
https://biblioteca.incaper.es.gov.br/digital/bitstream/123456789/3967/1/tomate-organico.pdf
|
|
Marc: |
LEADER 03380naa a2200337 a 4500
001 1022106
005 2020-12-14
008 2019 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.19084/rca.17142$2DOI
100 1 $aMAURI, A. L.
245 $aTratamentos sanitários na qualidade fisiológica e sanitária de sementes de tomate produzidas sob manejo orgânico.$h[electronic resource]
260 $c2019
520 $aO uso de sementes de alta qualidade fisiológica e sanitária representa importante estratégia na implantação de lavouras de tomate orgânico. Este trabalho foi realizado com o objetivo de avaliar o efeito de tratamentos sanitários na qualidade fisiológica e sanitária de sementes de tomate cereja produzidas sob manejo orgânico. Foram utilizados seis lotes de sementes de tomate cereja oriundos do Rio Grande do Sul e do Espírito Santo. As sementes foram submetidas a tratamentos sanitários alternativos que constaram de imersão das sementes em extratos vegetais (alfavaca-cravo e timbó) e em água destilada, microbiolização das sementes, termoterapia, além de um tratamento padrão com fungicida comercial e uma testemunha sem tratamento. Em seguida, as sementes foram avaliadas quanto à qualidade fisiológica e sanitária. O extrato de timbó reduziu a qualidade fisiológica das sementes de tomate. O tratamento de sementes com extrato de alfavaca-cravo e extrato de timbó inibiu a incidência de Penicillium sp. em sementes de tomate cereja, produzidas sob manejo orgânico. O extrato de alfavaca-cravo é eficiente na manutenção da qualidade fisiológica e sanitária das sementes de tomate cereja sob manejo orgânico. The use of high quality physiological and sanitary seeds represents an important strategy in the implantation of organic tomato crops. This work was carried out to evaluate the effect of sanitary treatments on physiological and sanitary quality of seeds of tomato grown under organic management. We used six seed lots of cherry tomatoes coming from Rio Grande do Sul and Espírito Santo. The seeds were subjected to alternative sanitary treatments that consisted of soaking seeds in plant extracts and in distilled water, seeds microbiolization, thermotherapy, in addition to standard treatment with commercial fungicide and a control without any treatment. Then the seeds were evaluated for physiological and sanitary quality. Timbó extract reduced the physiological quality of tomato seeds. The treatment of seeds with alfavaca-clove extract and timbó extract inhibited the incidence of Penicillium sp. in cherry tomato seeds, produced under organic management. The alfavaca-cravo extract is efficient in maintaining the physiological and sanitary quality of cherry tomato seeds under organic management.
650 $aGermination
650 $aOrganic farming
650 $aSeed production
650 $aAgricultura Orgânica
650 $aSemente
650 $aTeste de Sementes
650 $aTomate
653 $aAgricultura orgânica
653 $aGerminação
653 $aLycopersicon esculentum Mill
653 $aProdução de sementes
653 $aTomate
653 $aTomate orgânico
700 1 $aARAÚJO, E. F.
700 1 $aAMARO, H. T. R.
700 1 $aARAUJO, R. F.
700 1 $aPOSSE, S. C. P.
773 $tRevista de Ciências Agrárias$gv. 452, n. 4, p. 991-999, 2019.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Biblioteca Rui Tendinha (BRT) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
| Expressão de busca inválida. Verifique!!! |
|
|
