Data/hora: 16/06/2024 08:09:53

Biblioteca(s): 	Biblioteca Rui Tendinha.	
Data corrente: 	05/07/2020	
Data da última atualização: 	05/07/2020	
Autoria: 	MAURASTONI, M.; SÁ-ANTUNES, T. F.; OLIVEIRA, S.; SANTOS, A. M. C.; VENTURA, J. A.; FERNANDES, P. M. B.	
Afiliação: 	Marlonni Maurastoni, UFES; Tathiana F. Sá?Antunes, UFES; Scarlett A. Oliveira, UFES; Alexandre M. C. Santos, UFES; Jose Aires Ventura, Incaper; Patricia M. B. Fernandes, UFES.	
Título: 	A multiplex RT-PCR method to detect papaya meleira virus complex in adult pre-flowering plants.	
Ano de publicação: 	2020	
Fonte/Imprenta: 	Archives of Virology, v. 165, p. 1211-1214, july 2020.	
Idioma: 	Inglês	
Conteúdo: 	Papaya sticky disease (PSD), which can destroy orchards, was first attributed to papaya meleira virus (PMeV). However, the discovery of papaya meleira virus 2 (PMeV2) associated with PSD plants impose the need to detect this viral complex. We developed a multiplex RT-PCR (mPCR) technique capable of detecting two viruses in a single assay from pre-flowering plant samples, which is a useful tool for early diagnosis of PSD. We also determined the limit of detection (LOD) using asymmetric plasmid dilutions of both PMeV and PMeV2, which revealed that a higher titer of one virus prevents detection of the other. Thus, this technique is an alternative method for detecting PMeV and PMeV2 in a single reaction.	
Palavras-Chave: 	Doença das Planta; Multiplex; Papaya meleira virus (PMeV).	
Thesagro: 	Carica papaya; Doença de planta.	
Categoria do assunto: 	H Saúde e Patologia	
Registro original: 	Biblioteca Rui Tendinha (BRT)	
Exemplares: 	BRT - (23438-1) - UMT - AP - DD -  -  -  - DIS