| |
|
|
| Registros recuperados : 2 | |
| 1. |  | FERRÃO, M. A. G.; FONSECA, A. F. A. da.; FERRÃO, R. G.; MARRACCINI, P.; POT, D.; LEROY, T.; JR. BLOCH, C.; ANDRADE, A. C. Study of biological mechanims involved in drought tolerance in coffee plants. In: 22nd International Conference on Coffee Science, ASIC 2008, Campinas, SP, Brazil, 14-19 September, 2008. Association Scientifique Internationale du Café (ASIC), 2009. p. 874-881. p. 874-881| Biblioteca(s): Biblioteca Rui Tendinha. |
|    |
| 2. | | MARRACCINI, P.; VINECKY, F.; ALVES, G. S. C.; RAMOS, H. J. O.; ELBELT, S.; VIEIRA, N. G.; CARNEIRO, F. A.; SUJII, P. S.; ALEKCEVETCH, J. C.; SILVA, V. A.; DaMATTA, F. M.; FERRÃO, M. A. G.; LEROY, T.; POT, D.; VIEIRA, L. G. E.; SILVA, F. R. da; ANDRADE, A. C. Differentially expressed genes and proteins upon drought acclimation in tolerant and sensitive genotypes of Coffea canephora. Journal of Experimental Botany, Oxford v. 63, n. 11, p. 4191-4212, 2012.| Biblioteca(s): Biblioteca Rui Tendinha. |
|  |
| Registros recuperados : 2 | |
|
|
|
|
Registro Completo |
|
Biblioteca(s): |
Biblioteca Rui Tendinha. |
|
Data corrente: |
11/04/2024 |
|
Data da última atualização: |
11/04/2024 |
|
Autoria: |
FERRÃO, M. A. G.; FONSECA, A. F. A. da.; FERRÃO, R. G.; MARRACCINI, P.; POT, D.; LEROY, T.; JR. BLOCH, C.; ANDRADE, A. C. |
|
Afiliação: |
Maria Amélia Gava Ferrão, Incaper/Embrapa Café; Aymbiré Francisco Almeida da Fonseca, Incaper/Embrapa Café; Romário Gava Ferrão, Incaper; Pierre Marraccini; David Pot; Thierry Leroy; Carlos Jr. Bloch; Alan C. Andrade. |
|
Título: |
Study of biological mechanims involved in drought tolerance in coffee plants. |
|
Ano de publicação: |
2009 |
|
Fonte/Imprenta: |
In: 22nd International Conference on Coffee Science, ASIC 2008, Campinas, SP, Brazil, 14-19 September, 2008. Association Scientifique Internationale du Café (ASIC), 2009. p. 874-881. |
|
Páginas: |
p. 874-881 |
|
Idioma: |
Português |
|
Conteúdo: |
The principal aim of this study was to investigate the molecular mechanisms underlying the response to drought stress in coffee plants by different approaches. In order to identify candidate genes involved in controlling drought tolerance in coffee plants, different strategies were followed in our laboratories. The first used the nucleic data generated by the Brazilian Coffee EST project to identify candidate genes (CG) by in silico analysis (electronic Northern-blot). Differential expression of these CG was verified in leaves and roots from drought-tolerant and susceptible clones of C. canephora var. Conilon by Northern-blot and quantitative PCR experiments. The second was based on the screening of macroarray membranes spotted with coffee ESTs which were hybridized separately with leaf cDNA probes of the same clones. Finally, 2D gel electrophoresis was also performed to selected proteins presenting differential accumulation in leaves of the same clones. These proteins were analyzed by MALDI-TOF-MS/MS leading to the identification of a new set of CG. Results concerning the identification of CG by these different approaches are presented and discussed. |
|
Thesagro: |
Coffea Canephora; DNA; Gene. |
|
Categoria do assunto: |
-- |
|
URL: |
https://biblioteca.incaper.es.gov.br/digital/bitstream/item/4668/1/Study-of-Biological-Mechanims-Involved-in-Drought-Tolerance-in-Coffee-Plants.pdf
|
|
Marc: |
LEADER 01994naa a2200253 a 4500
001 1025586
005 2024-04-11
008 2009 bl uuuu u00u1 u #d
100 1 $aFERRÃO, M. A. G.
245 $aStudy of biological mechanims involved in drought tolerance in coffee plants.$h[electronic resource]
260 $c2009
300 $ap. 874-881
520 $aThe principal aim of this study was to investigate the molecular mechanisms underlying the response to drought stress in coffee plants by different approaches. In order to identify candidate genes involved in controlling drought tolerance in coffee plants, different strategies were followed in our laboratories. The first used the nucleic data generated by the Brazilian Coffee EST project to identify candidate genes (CG) by in silico analysis (electronic Northern-blot). Differential expression of these CG was verified in leaves and roots from drought-tolerant and susceptible clones of C. canephora var. Conilon by Northern-blot and quantitative PCR experiments. The second was based on the screening of macroarray membranes spotted with coffee ESTs which were hybridized separately with leaf cDNA probes of the same clones. Finally, 2D gel electrophoresis was also performed to selected proteins presenting differential accumulation in leaves of the same clones. These proteins were analyzed by MALDI-TOF-MS/MS leading to the identification of a new set of CG. Results concerning the identification of CG by these different approaches are presented and discussed.
650 $aCoffea Canephora
650 $aDNA
650 $aGene
700 1 $aFONSECA, A. F. A. da.
700 1 $aFERRÃO, R. G.
700 1 $aMARRACCINI, P.
700 1 $aPOT, D.
700 1 $aLEROY, T.
700 1 $aJR. BLOCH, C.
700 1 $aANDRADE, A. C.
773 $tIn: 22nd International Conference on Coffee Science, ASIC 2008, Campinas, SP, Brazil, 14-19 September, 2008. Association Scientifique Internationale du Café (ASIC), 2009. p. 874-881.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Biblioteca Rui Tendinha (BRT) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
| Expressão de busca inválida. Verifique!!! |
|
|
