02422naa a2200241 a 450000100080000000500110000800800410001910000180006024501260007826000090020452016910021365000180190465000220192265000250194465000420196970000210201170000220203270000200205470000190207470000240209370000240211777300390214110200182018-04-17       2018    bl uuuu     u00u1 u    #d1 aMADRONERO, J.  aTranscriptome analysis provides insights into the delayed sticky disease symptoms in Carica papayah[electronic resource]  c2018  aCarica papaya plants develop the papaya sticky disease (PSD) as a result of the combined infection of papaya meleira virus (PMeV) and papaya meleira virus 2 (PMeV2), or PMeV complex. PSD symptoms appear only after C. papaya flowers. To understand the mechanisms involved in this phenomenon, the global gene expression patterns of PMeV complex-infected C. papaya at pre-and post-flowering stages were assessed by RNA-Seq. The result was 633 and 88 differentially expressed genes at pre- and post-flowering stages, respectively. At pre-flowering stage, genes related to stress and transport were up-regulated while metabolism-related genes were down-regulated. It was observed that induction of several salicylic acid (SA)-activated genes, including PR1, PR2, PR5, WRKY transcription factors, ROS and callose genes, suggesting SA signaling involvement in the delayed symptoms. In fact, pre-flowering C. papaya treated with exogenous SA showed a tendency to decrease the PMeV and PMeV2 loads when compared to control plants. However, pre-flowering C. papaya also accumulated transcripts encoding a NPR1-inhibitor (NPR1-I/NIM1-I) candidate, genes coding for UDP-glucosyltransferases (UGTs) and several genes involved with ethylene pathway, known to be negative regulators of SA signaling. At post-flowering, when PSD symptoms appeared, the down-regulation of PR-1 encoding gene and the induction of BSMT1 and JA metabolism-related genes were observed. Hence, SA signaling likely operates at the pre-flowering stage of PMeV complex-infected C. papaya inhibiting the development of PSD symptoms, but the induction of its negative regulators prevents the full-scale and long-lasting tolerance.  aCarica papaya  aDefense responses  aPapaya meleira virus  aTranscriptome Plant'virus interaction1 aRODRIGUES, S. P.1 aANTUNES, T. F. S.1 aABREU, P. M. V.1 aVENTURA, J. A.1 aFERNANDES, A. A. R.1 aFERNANDES, P. M. B.  tPlant Cell Reports, p. 1-14, 2018.