01702naa a2200253 a 450000100080000000500110000800800410001910000120006024501320007226000090020452009580021365000120117165000150118365000180119865000200121665000310123665300290126770000140129670000160131070000190132670000140134570000160135977300730137510215382019-08-12       2013    bl uuuu     u00u1 u    #d1 aMELO, L  aA phytoplasma representative of a new subgroup, 16SrXIII-E, associated with Papaya apical curl necrosis.h[electronic resource]  c2013  aPapaya apical curl necrosis (PACN) has frequently been observed in several Brazilian states. Affected plants exhibit foliar chlorosis, curvature of the apex, shortening of the internodes leading to bunching of the crown leaves, necrosis of the young apical parts, leaf drop, and dieback. Naturally infected plants were sampled and subjected to PCR assays, which confirmed that a phytoplasma was associated with the disease. Sequencing of the 16S rRNA gene, conventional and computer-simulated RFLP analyses, and phylogenetic analysis allowed the determination of the PACN phytoplasma as a representative of a new subgroup, designated 16SrXIII-E. The phytoplasmas of various 16Sr groups, including 16SrI, 16SrII, 16SrX, 16SrXII, and 16SrXVII, are known to be involved in anomalies in papaya plants in several countries. However, the present study reports, for the first time, the occurrence of a 16SrXIII phytoplasma in association with a papaya disease.  aDieback  aMollicutes  aPapaya apical  aPhloem bacteria  aPhytoplasma classification  aEspírito Santo (Estado)1 aSILVA, E.1 aFLÔRES, D.1 aVENTURA, J. A.1 aCOSTA, H.1 aBEDENDO, I.  tEuropean Journal of Plant Pathologygv. 137, n. 3, p. 445-450, 2013.