02552naa a2200313 a 450000100080000000500110000800800410001910000360006024501240009626000090022052016510022965000190188065000120189965000110191165000260192265000250194865000140197365000230198765300120201065300140202265300210203665300220205770000180207970000160209770000190211370000240213270000240215677300580218010215402019-08-12       2010    bl uuuu     u00u1 u    #d1 aDE FARIAS VIÉGAS AGUIJE, G. M.  aCell wall alterations in the leaves of fusariosis-resistant and susceptible pineapple cultivars.h[electronic resource]  c2010  aFusariosis, caused by the fungus Fusarium subglutinans f. sp. ananas (Syn. F. guttiforme), is one of the main phytosanitary threats to pineapple (Ananas comosus var. comosus). Identification of plant cell responses to pathogens is important in understanding the plant?pathogen relationship and establishing strategies to improve and select resistant cultivars. Studies of the structural properties and phenolic content of cell walls in resistant (Vitoria) and susceptible (Perola) pineapple cultivars, related to resistance to the fungus, were performed. The non-chlorophyll base of physiologically mature leaves was inoculated with a conidia suspension. Analyses were performed post-inoculation by light, atomic force, scanning and transmission electron microscopy, and measurement of cell wall-bound phenolic compounds. Non-inoculated leaves were used as controls to define the constitutive tissue characteristics. Analyses indicated that morphological differences, such as cell wall thickness, cicatrization process and lignification, were related to resistance to the pathogen. Atomic force microscopy indicated a considerable difference in the mechanical properties of the resistant and susceptible cultivars, with more structural integrity, associated with higher levels of cell wall-bound phenolics, found in the resistant cultivar. p-Coumaric and ferulic acids were shown to be the major phenolics bound to the cell walls and were found in higher amounts in the resistant cultivar. Leaves of the resistant cultivar had reduced fungal penetration and a faster and more effective cicatrization response compared to the susceptible cultivar.  aAnanas comosus  aDisease  aFungus  aFusarium subglutinans  aParasite interaction  aPineapple  aResistant cultivar  aAbacaxi  aFusariose  aVariedade Perola  aVariedade Vitoria1 aZORZAL, P. B.1 aBUSS, D. S.1 aVENTURA, J. A.1 aFERNANDES, P. M. B.1 aFERNANDES, A. A. R.  tPlant Cell Reportsgv. 29, n. 10, p. 1109-1117, 2010.