01439naa a2200241 a 450000100080000000500110000800800410001910000190006024501230007926000090020252007140021165000180092565000220094365300230096565300140098865300320100270000230103470000170105770000210107470000190109570000240111477300590113810222332020-07-05       2020    bl uuuu     u00u1 u    #d1 aMAURASTONI, M.  aA multiplex RT-PCR method to detect papaya meleira virus complex in adult pre-flowering plants.h[electronic resource]  c2020  aPapaya sticky disease (PSD), which can destroy orchards, was first attributed to papaya meleira virus (PMeV). However, the discovery of papaya meleira virus 2 (PMeV2) associated with PSD plants impose the need to detect this viral complex. We developed a multiplex RT-PCR (mPCR) technique capable of detecting two viruses in a single assay from pre-flowering plant samples, which is a useful tool for early diagnosis of PSD. We also determined the limit of detection (LOD) using asymmetric plasmid dilutions of both PMeV and PMeV2, which revealed that a higher titer of one virus prevents detection of the other. Thus, this technique is an alternative method for detecting PMeV and PMeV2 in a single reaction.  aCarica papaya  aDoença de planta  aDoença das Planta  aMultiplex  aPapaya meleira virus (PMeV)1 aSÁ-ANTUNES, T. F.1 aOLIVEIRA, S.1 aSANTOS, A. M. C.1 aVENTURA, J. A.1 aFERNANDES, P. M. B.  tArchives of Virologygv. 165, p. 1211-1214, july 2020.