01445naa a2200229 a 450000100080000000500110000800800410001902400350006010000150009524501590011026000090026952007090027865000270098765000300101465000160104465000140106070000190107470000140109370000240110770000130113177300710114410066922015-10-23       2015    bl uuuu     u00u1 u    #d7 a10.1007/s40858-015-0027-72DOI1 aSANTOS, C.  aMALDI-TOF MS to identify the pineapple pathogen Fusarium guttiforme and its antagonist Trichoderma asperellum on decayed pineapple.h[electronic resource]  c2015  aMatrix-assisted laser desorption⁄ionisation time-offlight mass spectrometry (MALDI-TOF MS) has been used to identify some Fusarium and Trichoderma species, but early detection of fungal diseases by this technique has not yet been fully addressed. In this study, MALDI-TOF MS was tested to identify F. guttiforme on pineapple side shoots in situ. The efficacy of filamentous fungi for controlling fungal diseases is well documented. However, there is uncertainty whether the biocontrol agent is out growing the pathogen sufficiently to be identified. In this paper, a multistep identification of a plant pathogen (F. guttiforme) and its antagonist (T. asperellum) using MALDI-TOF MS is demonstrated.  aFungal early detection  aFungal ribosomal proteins  aMALDI-TOFMS  aPineapple1 aVENTURA, J. A.1 aCOSTA, H.1 aFERNANDES, P. M. B.1 aLIMA, N.  tTropical Plant Pathologygv. 40, Issue 4, p. 227-232, august 2015.