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http://biblioteca.incaper.es.gov.br/digital/handle/123456789/3087| Título: | Transcriptome analysis provides insights into the delayed sticky disease symptoms in Carica papaya |
| Autor(es): | MADRONERO, J.  RODRIGUES, S. P. ANTUNES, T. F. S. ABREU, P. M. V. VENTURA, J. A. FERNANDES, A. A. R. FERNANDES, P. M. B. Johana Madroñero, UFES; Silas P. Rodrigues, UFES; Tathiana F. S. Antunes, UFES; Paolla M. V. Abreu, UFES; Jose Aires Ventura, Incaper; A. Alberto R. Fernandes, UFES; Patricia Machado Bueno Fernandes, UFES. |
| Data do documento: | 17-Abr-2018 |
| Editor: | Plant Cell Reports, p. 1-14, 2018. |
| Descrição: | Carica papaya plants develop the papaya sticky disease (PSD) as a result of the combined infection of papaya meleira virus (PMeV) and papaya meleira virus 2 (PMeV2), or PMeV complex. PSD symptoms appear only after C. papaya flowers. To understand the mechanisms involved in this phenomenon, the global gene expression patterns of PMeV complex-infected C. papaya at pre-and post-flowering stages were assessed by RNA-Seq. The result was 633 and 88 differentially expressed genes at pre- and post-flowering stages, respectively. At pre-flowering stage, genes related to stress and transport were up-regulated while metabolism-related genes were down-regulated. It was observed that induction of several salicylic acid (SA)-activated genes, including PR1, PR2, PR5, WRKY transcription factors, ROS and callose genes, suggesting SA signaling involvement in the delayed symptoms. In fact, pre-flowering C. papaya treated with exogenous SA showed a tendency to decrease the PMeV and PMeV2 loads when compared to control plants. However, pre-flowering C. papaya also accumulated transcripts encoding a NPR1-inhibitor (NPR1-I/NIM1-I) candidate, genes coding for UDP-glucosyltransferases (UGTs) and several genes involved with ethylene pathway, known to be negative regulators of SA signaling. At post-flowering, when PSD symptoms appeared, the down-regulation of PR-1 encoding gene and the induction of BSMT1 and JA metabolism-related genes were observed. Hence, SA signaling likely operates at the pre-flowering stage of PMeV complex-infected C. papaya inhibiting the development of PSD symptoms, but the induction of its negative regulators prevents the full-scale and long-lasting tolerance. |
| URI: | http://biblioteca.incaper.es.gov.br/digital/handle/123456789/3087 |
| Aparece nas coleções: | Memória Técnica do Incaper |
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| BRT-biologicalaspectsandinsecticideactionofplantspecies-neves.pdf | 520,16 kB | Adobe PDF | Visualizar/Abrir |
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