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Campo DC | Valor | Idioma |
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dc.contributor.author | PERON, F. N. | |
dc.contributor.author | CALLONI, R. | |
dc.contributor.author | VENTURA, J. A. | |
dc.contributor.author | FERNANDES, P. M. B. | |
dc.contributor.other | UFES; UFGRS; Jose Aires Ventura, Incaper; UFES. | |
dc.date.accessioned | 2019-06-24T12:34:09Z | - |
dc.date.available | 2019-06-24T12:34:09Z | - |
dc.date.created | 2019 | |
dc.date.issued | 2019-06-24 | |
dc.identifier.other | 22610 | |
dc.identifier.uri | http://biblioteca.incaper.es.gov.br/digital/handle/123456789/3593 | - |
dc.description | Mealybug wilt of pineapple (MWP) is a disease caused by the Pineapple mealybug wilt-associated virus (PMWaV) complex transmitted by Dysmicoccus brevipes and D. neobrevipes. MWP symptoms are characterized by root dessication, leaf wilting and consequent failure to produce a fruit. The molecular mechanisms involved in the pineapple-PMWaV interaction for MWP symptomatology are still unclear. In this work, mRNAs of asymptomatic and symptomatic pineapple plants were evaluated using Illumina RNA sequencing technology. From a total of 79 million reads per sample, 16,097 genes were identified using STAR aligner and HTseq for paired-end files. Differentially expressed genes (DEGs) between the evaluated groups were estimated using DESeq2 and edgeR, with an FDR cutoff of ≤0.05. A total of 207 DEGs were detected, with 61 upregulated and 146 downregulated in symptomatic plants infected by PMWaV-2. The methodologies improved by the assays presented in this article and the detected DEGs can substantiate further researches with pineapple and the MWP disease. | |
dc.language | pt_BR | |
dc.publisher | Acta Horticulturae, 1239, p. 177-184, 2019. | |
dc.subject | Abacaxi | |
dc.title | Bioinformatics approach to the study of the molecular behaviour of mealybug wilt of pineapple. | |
dc.type | -- | |
dc.ainfo.id | 21431 | |
dc.ainfo.lastupdate | 2019-06-24 | |
dc.ainfo.depositante | Merielem Frasson | |
dc.subject.nalthesaurus | Bioinformatics | |
dc.subject.nalthesaurus | Pineapple | |
dc.subject.nalthesaurus | Transcriptomic | |
dc.subject.nalthesaurus | PMWaV | |
dc.subject.nalthesaurus | STAR | |
dc.subject.nalthesaurus | DESeq2 | |
dc.subject.nalthesaurus | EdgeR | |
dc.identifier.doi | 10.17660/ActaHortic.2019.1239.22 | |
Aparece nas coleções: | Memória Técnica do Incaper |
Arquivos associados a este item:
Arquivo | Descrição | Tamanho | Formato | |
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Bioinformatics-approach-to-the-molecular-study-Mealybug-wilt-pineapple-2019.pdf | 465,2 kB | Adobe PDF | Visualizar/Abrir |
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