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dc.contributor.authorMAURASTONI, M.
dc.contributor.authorSÁ-ANTUNES, T. F.
dc.contributor.authorOLIVEIRA, S.
dc.contributor.authorSANTOS, A. M. C.
dc.contributor.authorVENTURA, J. A.
dc.contributor.authorFERNANDES, P. M. B.
dc.contributor.otherMarlonni Maurastoni, UFES; Tathiana F. Sá?Antunes, UFES; Scarlett A. Oliveira, UFES; Alexandre M. C. Santos, UFES; Jose Aires Ventura, Incaper; Patricia M. B. Fernandes, UFES.
dc.date.accessioned2020-07-06T00:06:31Z-
dc.date.available2020-07-06T00:06:31Z-
dc.date.created2020
dc.date.issued2020-07-05
dc.identifier.other23438
dc.identifier.urihttp://biblioteca.incaper.es.gov.br/digital/handle/123456789/4040-
dc.descriptionPapaya sticky disease (PSD), which can destroy orchards, was first attributed to papaya meleira virus (PMeV). However, the discovery of papaya meleira virus 2 (PMeV2) associated with PSD plants impose the need to detect this viral complex. We developed a multiplex RT-PCR (mPCR) technique capable of detecting two viruses in a single assay from pre-flowering plant samples, which is a useful tool for early diagnosis of PSD. We also determined the limit of detection (LOD) using asymmetric plasmid dilutions of both PMeV and PMeV2, which revealed that a higher titer of one virus prevents detection of the other. Thus, this technique is an alternative method for detecting PMeV and PMeV2 in a single reaction.
dc.languageen
dc.publisherArch Virol, v. 165, p. 1211 1214, july 2020.
dc.subjectDoença das Planta
dc.subjectPapaya meleira virus (PMeV)
dc.subjectMultiplex
dc.titleA multiplex RT‑PCR method to detect papaya meleira virus complex in adult pre‑flowering plants.
dc.type--
dc.ainfo.id22233
dc.ainfo.lastupdate2020-07-05
dc.ainfo.depositanteMerielem Frasson
dc.subject.thesagroCarica papaya
dc.subject.thesagroDoença de planta
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