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dc.contributor.authorABREU, P. M. V.pt_BR
dc.contributor.authorPICCIN, J. G.pt_BR
dc.contributor.authorRODRIGUES, S. P.pt_BR
dc.contributor.authorVENTURA. J. A.pt_BR
dc.contributor.authorFERNANDES, P. M. B.pt_BR
dc.contributor.otherPaolla M.V. Abreu, UFES; João G. Piccin, UFES; Silas P. Rodrigues, UFES; Jose Aires Ventura, Incaper; Patricia M.B. Fernandes, UFES.pt_BR
dc.date.accessioned2014-01-08T11:07:57Z-
dc.date.available2014-01-08T11:07:57Z-
dc.date.created2012pt_BR
dc.date.issued2014-01-08pt_BR
dc.identifier.other1482pt_BR
dc.identifier.urihttp://ac.els-cdn.com/S0166093411004885/1-s2.0-S0166093411004885-main.pdf?_tid=597935f2-7857-11e3-a060-00000aacb35e&acdnat=1389180411_31ada3a608c8d17c0a26fd345cb062aept_BR
dc.identifier.urihttp://localhost:8080/digital/handle/item/162-
dc.identifier.urihttp://biblioteca.incaper.es.gov.br/digital/handle/item/162-
dc.descriptionPapaya meleira virus (PMeV) is the causal agent of papaya sticky disease. This study describes two methods for molecular diagnosis of PMeV using conventional and real-time PCR. These methods were shown to be more efficient than current methods of viral detection using extraction of PMeV dsRNA and observation of symptoms in the field. The methods described here were used to evaluate the effect of inoculation of papaya plants with purified PMeV dsRNA on the progress of PMeV infection. A single inoculation with PMeV dsRNA was observed to delay the progress of the virus infection by several weeks. The possibility of vertical transmission of PMeV was also investigated. No evidence was found for PMeV transmission through seeds collected from diseased fruit. The implications of these results for the epidemiology of PMeV and the management of papaya sticky disease are discussed.pt_BR
dc.languagept_BRpt_BR
dc.publisherCrop Protection, vol. 36, p. 7-10, 2012.pt_BR
dc.subjectPapaya meleira viruspt_BR
dc.subjectPapayapt_BR
dc.subjectResistance inductionpt_BR
dc.subjectPhytopathologypt_BR
dc.subjectDsRNApt_BR
dc.subjectReal-time RT-PCRpt_BR
dc.titleMolecular diagnosis of Papaya meleira virus (PMeV) from leaf samples of Carica papaya L. using conventional and real-time RT-PCR.pt_BR
dc.type--pt_BR
dc.ainfo.id1438pt_BR
dc.ainfo.lastupdate2014-01-08pt_BR
dc.ainfo.depositanteMerielem Frassonpt_BR
dc.format.extent27p.pt_BR
dc.identifier.doi10.1016/j.jviromet.2011.12.003pt_BR
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