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dc.contributor.authorCOSTA, H. B.pt_BR
dc.contributor.authorFERNANDES, P. M. B.pt_BR
dc.contributor.authorROMÃO, W.pt_BR
dc.contributor.authorVENTURA. J. A.pt_BR
dc.contributor.otherHelber B. Costa; Patricia M.B. Fernandes; Wanderson Romão; Jose Aires Ventura, Incaper.pt_BR
dc.date.accessioned2014-12-30T11:33:57Z-
dc.date.available2014-12-30T11:33:57Z-
dc.date.created2014pt_BR
dc.date.issued2014-12-30pt_BR
dc.identifier.other4847pt_BR
dc.identifier.urihttp://biblioteca.incaper.es.gov.br/digital/handle/item/419-
dc.descriptionBioproducts separation and purification processes are an important segment of the biotechnical industry. Bromelain is an enzyme which has great commercial value and is of wide interest in the pharmaceutical, alimentary, and textile industries, among others. The goal of this study was to develop a new method for bromelain purification from the stem residues resulting from agricultural processing of the pineapple plant. Bromelain was purified using two liquid chromatography steps, ion exchange plus gel filtration chromatography. Use of the methodology which was developed produced an enzyme with a molecular weight of 30 kDa (confirmed by SDS-PAGE), high recovery of enzymatic activity (89%), and with a purification factor of 16.93, a result superior to the methodologies described in the literature. HPLC showed the presence of two peaks in the ion exchange chromatogram and only one protein in the gel filtration chromatogram. Results indicate that, depending on the destination of the bromelain, the process can be stopped after the first purification step. The MALDI-TOF MS provided the peptide mass fingerprint of bromelain and MALDI-MS/MS the fragmentation profile and sequencing of the ions of m/z 951 and 1584. Thus, the connectivity and chemical structure of bromelain was confirmed. Moreover, besides its superiority to other methodologies, it can be applied to take advantage of the agricultural and industrial pineapple plant residues.pt_BR
dc.languagept_BRpt_BR
dc.publisherIndustrial Crops and Products, Vol. 59, August 2014, Pages 163-168.pt_BR
dc.titleA new procedure based on column chromatography to purify bromelain by ion exchange plus gel filtration chromatographies.pt_BR
dc.type--pt_BR
dc.ainfo.id4754pt_BR
dc.ainfo.lastupdate2014-12-30pt_BR
dc.ainfo.depositanteMerielem Frassonpt_BR
dc.format.extent26 p.pt_BR
dc.subject.nalthesaurusBromelainpt_BR
dc.subject.nalthesaurusEnzymept_BR
dc.subject.nalthesaurusPineapplept_BR
dc.subject.nalthesaurusPurificationpt_BR
dc.subject.nalthesaurusForbromelainpt_BR
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