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http://biblioteca.incaper.es.gov.br/digital/handle/item/4668| Título: | Study of biological mechanims involved in drought tolerance in coffee plants. |
| Autor(es): | FERRÃO, M. A. G.  FONSECA, A. F. A. da. FERRÃO, R. G. MARRACCINI, P. POT, D. LEROY, T. JR. BLOCH, C. ANDRADE, A. C. Maria Amélia Gava Ferrão, Incaper/Embrapa Café; Aymbiré Francisco Almeida da Fonseca, Incaper/Embrapa Café; Romário Gava Ferrão, Incaper; Pierre Marraccini; David Pot; Thierry Leroy; Carlos Jr. Bloch; Alan C. Andrade. |
| Data do documento: | 11-Abr-2024 |
| Editor: | In: 22nd International Conference on Coffee Science, ASIC 2008, Campinas, SP, Brazil, 14-19 September, 2008. Association Scientifique Internationale du Café (ASIC), 2009. p. 874-881. |
| Descrição: | The principal aim of this study was to investigate the molecular mechanisms underlying the response to drought stress in coffee plants by different approaches. In order to identify candidate genes involved in controlling drought tolerance in coffee plants, different strategies were followed in our laboratories. The first used the nucleic data generated by the Brazilian Coffee EST project to identify candidate genes (CG) by in silico analysis (electronic Northern-blot). Differential expression of these CG was verified in leaves and roots from drought-tolerant and susceptible clones of C. canephora var. Conilon by Northern-blot and quantitative PCR experiments. The second was based on the screening of macroarray membranes spotted with coffee ESTs which were hybridized separately with leaf cDNA probes of the same clones. Finally, 2D gel electrophoresis was also performed to selected proteins presenting differential accumulation in leaves of the same clones. These proteins were analyzed by MALDI-TOF-MS/MS leading to the identification of a new set of CG. Results concerning the identification of CG by these different approaches are presented and discussed. |
| URI: | http://biblioteca.incaper.es.gov.br/digital/handle/item/4668 |
| Aparece nas coleções: | Memória Técnica do Incaper |
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| Arquivo | Descrição | Tamanho | Formato | |
|---|---|---|---|---|
| Study-of-Biological-Mechanims-Involved-in-Drought-Tolerance-in-Coffee-Plants.pdf | 744,73 kB | Adobe PDF | Visualizar/Abrir |
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