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http://biblioteca.incaper.es.gov.br/digital/handle/item/5356| Título: | Advancing pineapple (Ananas comosus (L.) Merr.) cultivar identification and genetic diversity assessment by high resolution melting analysis and SSR markers. |
| Autor(es): | PEREIRA, P. R. C.  BILTES, R. VILLA, C. COSTA, J. VENTURA, J. A. BATITUCCI, M. do C. P. MAFRA, I. Paula Roberta Costalonga Pereira, REQUIMTE/LAQV; Rita Biltes, REQUIMTE/LAQV; Caterina Villa, REQUIMTE/LAQV; Joana Costa, REQUIMTE/LAQV; Jose Aires Ventura, Incaper; Maria do Carmo Pimentel Batitucci, UFES; Isabel Mafra, REQUIMTE/LAQV. |
| Data do documento: | 26-Mai-2026 |
| Editor: | Food Control, v. 189, 2026. |
| Descrição: | Pineapple (Ananas comosus var. comosus) is one of the most widely cultivated tropical fruits worldwide, with significant economic importance and genetic variability, particularly in Brazil. The identification and certification of cultivars are essential to ensure authenticity, traceability and quality in production. In this context, the present work aimed at developing a new molecular method using high resolution melting (HRM) analysis targeting simple sequence repeats (SSR) for variety and cultivar discrimination. The method allowed to discriminate seven characterised A. comosus genotypes into 4 independent clusters with high levels of confidence (≥98.7%): cluster 1 (reference cluster), including cultivars (cv.) Smooth Cayenne, Vitória, Pérola and Turiaçú; clusters 2 with cv. Primavera; cluster 3 with cv. Gigante de Tarauacá; and cluster 4 comprising the erectifolius variety. The number of the SSR repeating motif (GA)n is the main polymorphism responsible for discriminating A. comosus var. comosus from A. comosus var. erectifolius, while few single-nucleotide polymorphisms distinguished among var. comosus cultivars. The applicability of the HRM approach was successfully demonstrated, discriminating uncharacterised genotypes and commercial pineapple samples. Generally, the samples were logically discriminated according to their origins, which was corroborated by sequencing and melt curve profiles, highlighting the robustness of the method. This study represents the first application of HRM for differentiating A. comosus genotypes, offering a valuable tool for genetic resource management in pineapple production and food manufacturers. |
| URI: | http://biblioteca.incaper.es.gov.br/digital/handle/item/5356 |
| Aparece nas coleções: | Memória Técnica do Incaper |
Arquivos associados a este item:
| Arquivo | Descrição | Tamanho | Formato | |
|---|---|---|---|---|
| pineapple.pdf | 4,18 MB | Adobe PDF | Visualizar/Abrir |
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