Use este identificador para citar ou linkar para este item:
http://biblioteca.incaper.es.gov.br/digital/handle/item/5412| Título: | Production of PMWaV-free pineapple plants using combined thermotherapy and etiolation during micropropagation. |
| Autor(es): | PIASSI, M.  PERON, F. N. PIASSI, M. CAETANO, L. C. S. COSTA, A. F. da. FERNANDES, P. M. B. VENTURA, J. A. Mirian Piassi, Incaper; Fernanda Nunes Peron, UFES; Marcelo Piassi, IFMT; Luiz Carlos Santos Caetano, Incaper; Andrea Ferreira da Costa, Incaper; Patricia Machado Bueno Fernandes, UFES; Jose Aires Ventura, Incaper. |
| Palavras-chave: | Abacaxi Vitória |
| Data do documento: | 27-Out-2026 |
| Editor: | Plant Cell, Tissue and Organ Culture (PCTOC), v. 167, 2026. |
| Descrição: | Mealybug wilt of pineapple (MWP) is an economically critical viral disease affecting global pineapple production. In Brazil, MWP is associated with pineapple mealybug wilt-associated viruses (PMWaV-1, PMWaV-2, and PMWaV-3), which are primarily disseminated via vegetative propagation material, as infected plantlets often remain asymptomatic. In vitro propagation techniques provide a reliable approach to generate pathogen-free planting material. This study aimed to combine thermotherapy and etiolation with shoot tip culture to establish an optimized protocol for producing virus-free Vitória pineapple plants. In vitro shoots, multiplied from axillary buds of MWP-symptomatic Vitória pineapple plants, were subjected to heat treatments at 35, 37, and 39 (± 1) °C for 35 days, or incubated in continuous darkness for 30, 60, and 90 days to induce etiolation. Following thermotherapy or etiolation treatments, shoot tips (≈ 2.0 mm) were excised and cultured in vitro for 90 days prior to leaf sampling for viral detection by reverse transcription-quantitative PCR (RT-qPCR). Etiolation induction for 60 and 90 days resulted in the eradication of PMWaV-3. Thermotherapy at 39 (± 1) °C achieved the elimination of PMWaV-1 and PMWaV-3 across all tested samples, and PMWaV-2 in 67% of samples, with a shoot tip regeneration of 37%. Virus-free shoots were subsequently multiplied in vitro and acclimatized in a greenhouse before field transplantation, where they remained healthy over two production cycles. The developed protocol provides a valuable method for large-scale production of virus-free planting material, meeting growers demands in a cost-effective manner. |
| URI: | http://biblioteca.incaper.es.gov.br/digital/handle/item/5412 |
| Aparece nas coleções: | Memória Técnica do Incaper |
Arquivos associados a este item:
| Arquivo | Descrição | Tamanho | Formato | |
|---|---|---|---|---|
| s11240-026-03633-6.pdf | 8,23 MB | Adobe PDF | Visualizar/Abrir |
Os itens no repositório estão protegidos por copyright, com todos os direitos reservados, salvo quando é indicado o contrário.