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Use este identificador para citar ou linkar para este item: http://biblioteca.incaper.es.gov.br/digital/handle/item/541
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dc.contributor.authorMARRACCINI, P.pt_BR
dc.contributor.authorVINECKY, F.pt_BR
dc.contributor.authorALVES, G. S. C.pt_BR
dc.contributor.authorRAMOS, H. J. O.pt_BR
dc.contributor.authorELBELT, S.pt_BR
dc.contributor.authorVIEIRA, N. G.pt_BR
dc.contributor.authorCARNEIRO, F. A.pt_BR
dc.contributor.authorSUJII, P. S.pt_BR
dc.contributor.authorALEKCEVETCH, J. C.pt_BR
dc.contributor.authorSILVA, V. A.pt_BR
dc.contributor.authorDaMATTA, F. M.pt_BR
dc.contributor.authorFERRÃO, M. A. G.pt_BR
dc.contributor.authorLEROY, T.pt_BR
dc.contributor.authorPOT, D.pt_BR
dc.contributor.authorVIEIRA, L. G. E.pt_BR
dc.contributor.authorSILVA, F. R. dapt_BR
dc.contributor.authorANDRADE, A. C.pt_BR
dc.contributor.otherMaria Amélia Gava Ferrão, Incaper/Embrapa Café.pt_BR
dc.date.accessioned2015-01-13T18:09:39Z-
dc.date.available2015-01-13T18:09:39Z-
dc.date.created2012pt_BR
dc.date.issued2015-01-13pt_BR
dc.identifier.other5004pt_BR
dc.identifier.urihttp://biblioteca.incaper.es.gov.br/digital/handle/item/541-
dc.descriptionThe aim of this study was to investigate the molecular mechanisms underlying drought acclimation in coffee plants by the identification of candidate genes (CGs) using different approaches. The first approach used the data generated during the Brazilian Coffee expressed sequence tag (EST) project to select 13 CGs by an in silico analysis (electronic northern). The second approach was based on screening macroarrays spotted with plasmid DNA (coffee ESTs) with separate hybridizations using leaf cDNA probes from drought-tolerant and susceptible clones of Coffea canephora var. Conilon, grown under different water regimes. This allowed the isolation of seven additional CGs. The third approach used two-dimensional gel electrophoresis to identify proteins displaying differential accumulation in leaves of drought-tolerant and susceptible clones of C. canephora. Six of them were characterized by MALDI-TOF-MS/MS (matrix-assisted laser desorption-time of flight-tandem mass spectrometry) and the corresponding proteins were identified. Finally, additional CGs were selected from the literature, and quantitative real-time polymerase chain reaction (qPCR) was performed to analyse the expression of all identified CGs. Altogether, >40 genes presenting differential gene expression during drought acclimation were identified, some of them showing different expression profiles between drought-tolerant and susceptible clones. Based on the obtained results, it can be concluded that factors involved a complex network of responses probably involving the abscisic signalling pathway and nitric oxide are major molecular determinants that might explain the better efficiency in controlling stomata closure and transpiration displayed by drought-tolerant clones of C. canephora.pt_BR
dc.languagept_BRpt_BR
dc.publisherJournal of Experimental Botany, Oxford v. 63, n. 11, p. 4191-4212, 2012.pt_BR
dc.subjectCandidate genept_BR
dc.subjectCoffea canephorapt_BR
dc.subjectDifferential expressionpt_BR
dc.subjectDrought acclimationpt_BR
dc.subjectGenéticapt_BR
dc.subjectProteomicpt_BR
dc.subjectProteômicapt_BR
dc.subjectReal time PCRpt_BR
dc.titleDifferentially expressed genes and proteins upon drought acclimation in tolerant and sensitive genotypes of Coffea canephora.pt_BR
dc.type--pt_BR
dc.ainfo.id4907pt_BR
dc.ainfo.lastupdate2015-01-13pt_BR
dc.ainfo.depositanteMerielem Frassonpt_BR
dc.subject.thesagroCoffea canephorapt_BR
dc.subject.thesagroGeneticspt_BR
dc.subject.thesagroCafépt_BR
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