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Registro Completo |
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Biblioteca(s): |
Biblioteca Rui Tendinha. |
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Data corrente: |
08/08/2019 |
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Data da última atualização: |
08/08/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
FERRÃO, L. F. V.; CAIXETA, E. T.; CRUZ, C. D.; SOUZA, F. de F.; FERRÃO, M. A. G.; ZAMBOLIM, Z.; SAKIYAMA, N. S. |
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Afiliação: |
Luís Felipe V. Ferrão, UFV; Eveline T. Caixeta, Embrapa Cafe; Cosme D. Cruz, UFV; Flávio F. de Souza, Embrapa Rondônia; Maria Amélia Gava Ferrão, Incaper/Embrapa Café; Laércio Zambolim, UFV; Ney S. Sakiyama, UFV. |
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Título: |
The effects of encoding data in diversity studies and the applicability of the weighting index approach for data analysis from different molecular markers. |
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Ano de publicação: |
2014 |
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Fonte/Imprenta: |
Plant Systematics and Evolution, New York v. 300, p.1649-1661, feb. 2014. |
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DOI: |
10.1007/s00606-014-09903 |
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Idioma: |
Português |
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Conteúdo: |
The use of molecular markers to study genetic diversity represents a breakthrough in this area, because of the increase in polymorphism levels and phenotypic neutrality. Codominant markers, such as microsatellites (SSR), are sensitive enough to distinguish the heterozygotes in genetic studies. Despite this advantage, there are some studies that ignore this feature and work with encoded data because of the simplicity of the evaluation, existence of polyploids and need for the combined analysis of different types of molecular markers. Thus, our study aims to investigate the consequences of these encodings on simulated and real data. In addition, we suggest an alternative analysis for genetic evaluations using different molecular markers. For the simulated data, we proposed the following two scenarios: the first uses SNP markers, and the second SSR markers. For real data, we used the SSR genotyping data from Coffea canephora accessions maintained in the Embrapa Germplasm Collection. The genetic diversity was studied using cluster analysis, the dissimilarity index, and the Bayesian approach implemented in the STRUCTURE software. For the simulated data, we observed a loss of genetic information to the encoded data in both scenarios. The same result was observed in the coffee studies. This loss of information was discussed in the context of a plantbreeding program, and the consequences were weighted to germplasm evaluations and the selection of parents for hybridization. In the studies that involved different types of markers, an alternative to the combined analysis is discussed, where the informativeness, coverage and quality of markers are weighted in the genetic diversity studies. MenosThe use of molecular markers to study genetic diversity represents a breakthrough in this area, because of the increase in polymorphism levels and phenotypic neutrality. Codominant markers, such as microsatellites (SSR), are sensitive enough to distinguish the heterozygotes in genetic studies. Despite this advantage, there are some studies that ignore this feature and work with encoded data because of the simplicity of the evaluation, existence of polyploids and need for the combined analysis of different types of molecular markers. Thus, our study aims to investigate the consequences of these encodings on simulated and real data. In addition, we suggest an alternative analysis for genetic evaluations using different molecular markers. For the simulated data, we proposed the following two scenarios: the first uses SNP markers, and the second SSR markers. For real data, we used the SSR genotyping data from Coffea canephora accessions maintained in the Embrapa Germplasm Collection. The genetic diversity was studied using cluster analysis, the dissimilarity index, and the Bayesian approach implemented in the STRUCTURE software. For the simulated data, we observed a loss of genetic information to the encoded data in both scenarios. The same result was observed in the coffee studies. This loss of information was discussed in the context of a plantbreeding program, and the consequences were weighted to germplasm evaluations and the selection of parents for hybridization. In the st... Mostrar Tudo |
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Palavras-Chave: |
Café canéfora; Café conilon; Codominant markers; Coffee; Diversidade genética; Dominant markers Germplasm; Marcadores dominantes; SSR STRUCTURE. |
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Thesagro: |
Café; Coffea Canephora; Marcador molecular. |
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Categoria do assunto: |
-- |
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URL: |
https://biblioteca.incaper.es.gov.br/digital/bitstream/123456789/3664/1/the-effects-enconding-ferrao.pdf
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Marc: |
LEADER 02777naa a2200337 a 4500
001 1021517
005 2019-08-08
008 2014 bl uuuu u00u1 u #d
024 7 $a10.1007/s00606-014-09903$2DOI
100 1 $aFERRÃO, L. F. V.
245 $aThe effects of encoding data in diversity studies and the applicability of the weighting index approach for data analysis from different molecular markers.$h[electronic resource]
260 $c2014
520 $aThe use of molecular markers to study genetic diversity represents a breakthrough in this area, because of the increase in polymorphism levels and phenotypic neutrality. Codominant markers, such as microsatellites (SSR), are sensitive enough to distinguish the heterozygotes in genetic studies. Despite this advantage, there are some studies that ignore this feature and work with encoded data because of the simplicity of the evaluation, existence of polyploids and need for the combined analysis of different types of molecular markers. Thus, our study aims to investigate the consequences of these encodings on simulated and real data. In addition, we suggest an alternative analysis for genetic evaluations using different molecular markers. For the simulated data, we proposed the following two scenarios: the first uses SNP markers, and the second SSR markers. For real data, we used the SSR genotyping data from Coffea canephora accessions maintained in the Embrapa Germplasm Collection. The genetic diversity was studied using cluster analysis, the dissimilarity index, and the Bayesian approach implemented in the STRUCTURE software. For the simulated data, we observed a loss of genetic information to the encoded data in both scenarios. The same result was observed in the coffee studies. This loss of information was discussed in the context of a plantbreeding program, and the consequences were weighted to germplasm evaluations and the selection of parents for hybridization. In the studies that involved different types of markers, an alternative to the combined analysis is discussed, where the informativeness, coverage and quality of markers are weighted in the genetic diversity studies.
650 $aCafé
650 $aCoffea Canephora
650 $aMarcador molecular
653 $aCafé canéfora
653 $aCafé conilon
653 $aCodominant markers
653 $aCoffee
653 $aDiversidade genética
653 $aDominant markers Germplasm
653 $aMarcadores dominantes
653 $aSSR STRUCTURE
700 1 $aCAIXETA, E. T.
700 1 $aCRUZ, C. D.
700 1 $aSOUZA, F. de F.
700 1 $aFERRÃO, M. A. G.
700 1 $aZAMBOLIM, Z.
700 1 $aSAKIYAMA, N. S.
773 $tPlant Systematics and Evolution, New York$gv. 300, p.1649-1661, feb. 2014.
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Registro original: |
Biblioteca Rui Tendinha (BRT) |
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Biblioteca |
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Classificação |
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Registro |
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Registro Completo |
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Biblioteca(s): |
Biblioteca Rui Tendinha. |
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Data corrente: |
28/06/2016 |
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Data da última atualização: |
28/06/2016 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
- - - |
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Autoria: |
ZAMBOLIM, L.; SOUZA NETO, P. N. de.; ZAMBOLIM, E. M.; CAIXETA, E. T.; SAKIYAMA, N. S.; FERRÃO, R. G. |
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Afiliação: |
Laércio Zambolim, UFV; Pedro Nery de Souza Neto, UFV; Eunize Maciel Zambolim, UFV; Eveline Teixeira Caixeta, EMBRAPA; Ney Sussumo Sakiyama, UFV; Romário Gava Ferrão, Incaper. |
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Título: |
Components of resistance of conilon coffee that reduce the rate of leaf rust development. |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
Australasian Plant Pathology, p. 1-12, june 2016. |
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ISSN: |
1448-6032 |
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DOI: |
10.1007/s13313-016-0425-4 |
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Idioma: |
Inglês |
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Conteúdo: |
This study aimed to quantify the levels of resistance of 39 hybrids ?Clone 83 × Clone 02?of Coffea canephora var. conilon to the races II (v5) and XXXIII (v5,7,9) of H. vastatrix. To study the resistance of the genotypes, leaf discs were removed from the plants and spreaded on the top of a stainless steel screen inside gerbox as a moist chamber. The gerboxes were mantained at 22 ± 2 °C in the dark for 48 h and under 12 h of photoperiod, respectively. The hybrids were classified into four levels: resistant (R), moderately resistant (MR), moderately susceptible (MS) and susceptible (S). Leaf discs (%) containing sporulated lesions was the resistance component that had the greatest absolute correlation (0.95). The level of resistance of the hybrids varyied according to the race of the pathogen. The percentage of R, MR, MS and S of the hybrids to race II and XXXIII were 25 %, 25 %, 38 %, and 11 %, and 13 %, 37 %, 42 %, and 8 %, respectively. Nine (25 %) and five (13 %) hybrids were R to race II and XXXIII, respectively. Four hybrids (21, 30, 48 and 66) showed vertical resistance to races II and XXXIII. Possibly these hybrids have the resistance factor SH6, because they were resistant to race XXXIII v5,7,9. Leaf discs (%) with sporulating lesions can be used as a reference in future studyies of screening genotypes with horizontal resistance to C. canephora genotypes to leaf rust. |
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Palavras-Chave: |
Café Conilon. |
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Thesaurus NAL: |
Coffea canephora; Hemileia vastatrix; Horizontal resistance; Physiological races. |
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Categoria do assunto: |
G Melhoramento Genético |
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URL: |
http://link.springer.com/article/10.1007/s13313-016-0425-4
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Marc: |
LEADER 02155nam a2200253 a 4500
001 1011126
005 2016-06-28
008 2016 bl uuuu u0uu1 u #d
022 $a1448-6032
024 7 $a10.1007/s13313-016-0425-4$2DOI
100 1 $aZAMBOLIM, L.
245 $aComponents of resistance of conilon coffee that reduce the rate of leaf rust development.
260 $aAustralasian Plant Pathology, p. 1-12, june 2016.$c2016
520 $aThis study aimed to quantify the levels of resistance of 39 hybrids ?Clone 83 × Clone 02?of Coffea canephora var. conilon to the races II (v5) and XXXIII (v5,7,9) of H. vastatrix. To study the resistance of the genotypes, leaf discs were removed from the plants and spreaded on the top of a stainless steel screen inside gerbox as a moist chamber. The gerboxes were mantained at 22 ± 2 °C in the dark for 48 h and under 12 h of photoperiod, respectively. The hybrids were classified into four levels: resistant (R), moderately resistant (MR), moderately susceptible (MS) and susceptible (S). Leaf discs (%) containing sporulated lesions was the resistance component that had the greatest absolute correlation (0.95). The level of resistance of the hybrids varyied according to the race of the pathogen. The percentage of R, MR, MS and S of the hybrids to race II and XXXIII were 25 %, 25 %, 38 %, and 11 %, and 13 %, 37 %, 42 %, and 8 %, respectively. Nine (25 %) and five (13 %) hybrids were R to race II and XXXIII, respectively. Four hybrids (21, 30, 48 and 66) showed vertical resistance to races II and XXXIII. Possibly these hybrids have the resistance factor SH6, because they were resistant to race XXXIII v5,7,9. Leaf discs (%) with sporulating lesions can be used as a reference in future studyies of screening genotypes with horizontal resistance to C. canephora genotypes to leaf rust.
650 $aCoffea canephora
650 $aHemileia vastatrix
650 $aHorizontal resistance
650 $aPhysiological races
653 $aCafé Conilon
700 1 $aSOUZA NETO, P. N. de.
700 1 $aZAMBOLIM, E. M.
700 1 $aCAIXETA, E. T.
700 1 $aSAKIYAMA, N. S.
700 1 $aFERRÃO, R. G.
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Biblioteca Rui Tendinha (BRT) |
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