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Registro Completo |
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Biblioteca(s): |
Biblioteca Rui Tendinha. |
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Data corrente: |
30/12/2014 |
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Data da última atualização: |
01/09/2015 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
COSTA, H. B.; FERNANDES, P. M. B.; ROMÃO, W.; VENTURA, J. A. |
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Afiliação: |
Helber B. Costa; Patricia M.B. Fernandes; Wanderson Romão; Jose Aires Ventura, Incaper. |
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Título: |
A new procedure based on column chromatography to purify bromelain by ion exchange plus gel filtration chromatographies. |
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Ano de publicação: |
2014 |
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Fonte/Imprenta: |
Industrial Crops and Products, Vol. 59, p. 163-168, august 2014. |
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Páginas: |
6 p. |
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Idioma: |
Inglês |
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Conteúdo: |
Bioproducts separation and purification processes are an important segment of the biotechnical industry. Bromelain is an enzyme which has great commercial value and is of wide interest in the pharmaceutical, alimentary, and textile industries, among others. The goal of this study was to develop a new method for bromelain purification from the stem residues resulting from agricultural processing of the pineapple plant. Bromelain was purified using two liquid chromatography steps, ion exchange plus gel filtration chromatography. Use of the methodology which was developed produced an enzyme with a molecular weight of 30 kDa (confirmed by SDS-PAGE), high recovery of enzymatic activity (89%), and with a purification factor of 16.93, a result superior to the methodologies described in the literature. HPLC showed the presence of two peaks in the ion exchange chromatogram and only one protein in the gel filtration chromatogram. Results indicate that, depending on the destination of the bromelain, the process can be stopped after the first purification step. The MALDI-TOF MS provided the peptide mass fingerprint of bromelain and MALDI-MS/MS the fragmentation profile and sequencing of the ions of m/z 951 and 1584. Thus, the connectivity and chemical structure of bromelain was confirmed. Moreover, besides its superiority to other methodologies, it can be applied to take advantage of the agricultural and industrial pineapple plant residues. |
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Thesaurus NAL: |
Bromelain; Enzyme; Forbromelain; Pineapple; Purification. |
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Categoria do assunto: |
-- |
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URL: |
http://biblioteca.incaper.es.gov.br/digital/bitstream/item/419/1/New-Procedure-Chromatography-to-Purify-Bromelain-2014.pdf
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Marc: |
LEADER 02130naa a2200229 a 4500
001 1004754
005 2015-09-01
008 2014 bl uuuu u00u1 u #d
100 1 $aCOSTA, H. B.
245 $aA new procedure based on column chromatography to purify bromelain by ion exchange plus gel filtration chromatographies.$h[electronic resource]
260 $c2014
300 $a6 p.
520 $aBioproducts separation and purification processes are an important segment of the biotechnical industry. Bromelain is an enzyme which has great commercial value and is of wide interest in the pharmaceutical, alimentary, and textile industries, among others. The goal of this study was to develop a new method for bromelain purification from the stem residues resulting from agricultural processing of the pineapple plant. Bromelain was purified using two liquid chromatography steps, ion exchange plus gel filtration chromatography. Use of the methodology which was developed produced an enzyme with a molecular weight of 30 kDa (confirmed by SDS-PAGE), high recovery of enzymatic activity (89%), and with a purification factor of 16.93, a result superior to the methodologies described in the literature. HPLC showed the presence of two peaks in the ion exchange chromatogram and only one protein in the gel filtration chromatogram. Results indicate that, depending on the destination of the bromelain, the process can be stopped after the first purification step. The MALDI-TOF MS provided the peptide mass fingerprint of bromelain and MALDI-MS/MS the fragmentation profile and sequencing of the ions of m/z 951 and 1584. Thus, the connectivity and chemical structure of bromelain was confirmed. Moreover, besides its superiority to other methodologies, it can be applied to take advantage of the agricultural and industrial pineapple plant residues.
650 $aBromelain
650 $aEnzyme
650 $aForbromelain
650 $aPineapple
650 $aPurification
700 1 $aFERNANDES, P. M. B.
700 1 $aROMÃO, W.
700 1 $aVENTURA, J. A.
773 $tIndustrial Crops and Products, Vol. 59, p. 163-168, august 2014.
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Registro original: |
Biblioteca Rui Tendinha (BRT) |
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| Registros recuperados : 354 | |
| 3. |  | UENO, B.; COSTA, H. Doenças causadas por fungos e bactérias. In: ANTUNES, L. E. C.; REISSER JUNIOR, C.; SCHWENGBER, J. E. (Ed.). Morangueiro. Pelotas: Embrapa Clima Temperado: Brasília, DF: Embrapa, 2016. 589 p. p. 413-480| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Biblioteca Rui Tendinha. |
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| 12. | | COSTA, H.; VENTURA, J. A. Doenças do gengibre. In: CARMO, C. A. S. do.; BALBINO, J. M. de S. (Org.). Gengibre. Vitória, ES : Incaper, 2015. 190 p.| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Biblioteca Rui Tendinha. |
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| 13. | | VENTURA, J. A.; COSTA, H. Doenças da mandioquinha-salsa. In: SANTOS, F.F. dos; CARMO, C.A.S. do., ed. Mandioquinha-salsa: manejo cultural. Brasilia: EMBRAPA-SPI / EMBRAPA-CNPH, 1998. p. 50-56.| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Biblioteca Rui Tendinha. |
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| Registros recuperados : 354 | |
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