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Registro Completo |
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Biblioteca(s): |
Biblioteca Rui Tendinha. |
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Data corrente: |
06/07/2022 |
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Data da última atualização: |
06/07/2022 |
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Tipo da produção científica: |
Publicação em Anais de Congresso |
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Autoria: |
SOUZA, C. A. S.; AGUILAR, M. A. G.; DIAS, L. A. dos S.; SANTOS, L. C.; DA VITÓRIA, Y. T.; COSTA, L. L. |
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Afiliação: |
Carlos Alberto Spaggiari Souza, CEPLAC/ES; Marco Antonio Galeas Aguilar, CEPLAC/ES; Luiz Antonio dos Santos Dias, UFV; Lucas Calazans Santos, Incaper; Yago Tonini da Vitória; Lucas Leite Costa. |
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Título: |
Seleção de genótipos para o estudo da interação enxerto x portaenxerto em mudas de cacaueiro. |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
In: CONGRESSO CAPIXABA DE PESQUISA AGROPECUÁRIA, 1., Vitória, ES. Anais 2021 : congresso capixaba de pesquisa agropecuária [recurso eletrônico]. Vitória, ES: Incaper, 2021. color. PDF ; 25,4 MB. E-book, no formato PDF. (Incaper, Documentos, 289). Pedro Luís Pereira Teixeira de Carvalho, Carlos Henrique Rodrigues de Oliveira, José Aires Ventura, Marcos Vinicius Winckler Caldeira e Romário Gava Ferrão, editores. |
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Páginas: |
p. 165-168 |
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Idioma: |
Português |
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Conteúdo: |
O objetivo desta proposta de pesquisa foi selecionar novos genótipos para ampliar a base genética e o número de clones de cacaueiros a serem usados para plantio, visando à renovação e a revitalização da cacauicultura capixaba. Foram selecionados e testados 65 genótipos de cacaueiro, com potencial para serem usados como porta-enxertos. Os dois experimentos foram instalados na Estação Experimental Filogônio Peixoto ? ESFIP, pertencente à CEPLAC, em Linhares-ES, delineamento Blocos Casualizados, quatro repetições e com nove plantas por parcela. Os experimentos foram constituídos de 30 e 35 genótipos, respectivamente. Cada genótipo foi testado como um porta-enxerto (raiz). Como enxerto (parte aérea), o PS-1319, que é o material genético resistente à vassoura-de-bruxa, mais plantado no Estado do Espírito Santo. Os genótipos TSH-1188, ESFIP-03, ESFIP-04, SCS20, Maranhão, FA-13, Salobrinho-3, PS-1030, FMB-15, Cepec-42, CP-40, uma planta da Fazenda Timbuí e dois Híbridos F1: provenientes do cruzamento de polinização aberta de Sca-6 x Catongo, e de TSH-1888 x cacau comum, podem ser considerados os mais promissores para porta-enxerto de cacaueiro, além de ampliarem a base genética dos materiais usados. No entanto, precisam ser validados em condições de campo, pois o cacaueiro é um cultivo perene, onde a produção comercial só começa a partir do quarto ano de plantio. |
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Palavras-Chave: |
Espírito Santo (Estado); Planta matrize; Produção de muda; Variabilidade genética. |
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Thesagro: |
Cacau; Cacauicultura; Clone; Enxerto; Genética; Genética Vegetal; Matriz; Muda; Porta Enxerto; Theobroma Cacao; Variação Genética. |
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Categoria do assunto: |
G Melhoramento Genético |
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URL: |
https://biblioteca.incaper.es.gov.br/digital/bitstream/item/4154/1/Anais-CCPA-165-168.pdf
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Marc: |
LEADER 02808nam a2200361 a 4500
001 1023884
005 2022-07-06
008 2022 bl uuuu u01u1 u #d
100 1 $aSOUZA, C. A. S.
245 $aSeleção de genótipos para o estudo da interação enxerto x portaenxerto em mudas de cacaueiro.$h[electronic resource]
260 $aIn: CONGRESSO CAPIXABA DE PESQUISA AGROPECUÁRIA, 1., Vitória, ES. Anais 2021 : congresso capixaba de pesquisa agropecuária [recurso eletrônico]. Vitória, ES: Incaper, 2021. color. PDF ; 25,4 MB. E-book, no formato PDF. (Incaper, Documentos, 289). Pedro Luís Pereira Teixeira de Carvalho, Carlos Henrique Rodrigues de Oliveira, José Aires Ventura, Marcos Vinicius Winckler Caldeira e Romário Gava Ferrão, editores.$c2022
300 $ap. 165-168
520 $aO objetivo desta proposta de pesquisa foi selecionar novos genótipos para ampliar a base genética e o número de clones de cacaueiros a serem usados para plantio, visando à renovação e a revitalização da cacauicultura capixaba. Foram selecionados e testados 65 genótipos de cacaueiro, com potencial para serem usados como porta-enxertos. Os dois experimentos foram instalados na Estação Experimental Filogônio Peixoto ? ESFIP, pertencente à CEPLAC, em Linhares-ES, delineamento Blocos Casualizados, quatro repetições e com nove plantas por parcela. Os experimentos foram constituídos de 30 e 35 genótipos, respectivamente. Cada genótipo foi testado como um porta-enxerto (raiz). Como enxerto (parte aérea), o PS-1319, que é o material genético resistente à vassoura-de-bruxa, mais plantado no Estado do Espírito Santo. Os genótipos TSH-1188, ESFIP-03, ESFIP-04, SCS20, Maranhão, FA-13, Salobrinho-3, PS-1030, FMB-15, Cepec-42, CP-40, uma planta da Fazenda Timbuí e dois Híbridos F1: provenientes do cruzamento de polinização aberta de Sca-6 x Catongo, e de TSH-1888 x cacau comum, podem ser considerados os mais promissores para porta-enxerto de cacaueiro, além de ampliarem a base genética dos materiais usados. No entanto, precisam ser validados em condições de campo, pois o cacaueiro é um cultivo perene, onde a produção comercial só começa a partir do quarto ano de plantio.
650 $aCacau
650 $aCacauicultura
650 $aClone
650 $aEnxerto
650 $aGenética
650 $aGenética Vegetal
650 $aMatriz
650 $aMuda
650 $aPorta Enxerto
650 $aTheobroma Cacao
650 $aVariação Genética
653 $aEspírito Santo (Estado)
653 $aPlanta matrize
653 $aProdução de muda
653 $aVariabilidade genética
700 1 $aAGUILAR, M. A. G.
700 1 $aDIAS, L. A. dos S.
700 1 $aSANTOS, L. C.
700 1 $aDA VITÓRIA, Y. T.
700 1 $aCOSTA, L. L.
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Registro original: |
Biblioteca Rui Tendinha (BRT) |
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Biblioteca |
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Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
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Fechar
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Registro Completo |
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Biblioteca(s): |
Biblioteca Rui Tendinha. |
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Data corrente: |
09/09/2020 |
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Data da última atualização: |
09/09/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
DUARTE, M. F.; PEREIRA-CARVALHO, R. de C.; REIS, L. de N. A. dos; ROJAS, M. J.; GILBERTSON, R.; COSTA, H.; BOITEUX, L. S.; FONSECA, M. E. N. |
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Afiliação: |
Macaria Ferreira Duarte, UnB; Rita de Cássia Pereira Carvalho, UnB; Luciane de Nazaré Almeida dos Reis, UnB; Maria J. Rojas, UC-DAVIS; Robert Gilbertson, UC-DAVIS; Helcio Costa, Incaper; Leonardo Silva Boiteux, EMBRAPA; Maria Esther N. Fonseca, EMBRAPA. |
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Título: |
Natural Infection of Tomatoes (Solanum lycopersicum) by Euphorbia yellow mosaic virus Isolates across Four Brazilian States. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Plant Disease, set. 2020. |
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Idioma: |
Inglês |
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Conteúdo: |
Severe yield losses induced by a complex of whitefly?transmitted Begomovirus species (family Geminiviridae) have been reported in tomatoes in Brazil (Reis et al. 2020). Nine isolates were obtained from tomato plants exhibiting begomovirus?like symptoms (viz. apical and interveinal chlorosis, yellow spots, and stunting) during independent field surveys: one isolate in Sumaré, São Paulo?SP State (isolate SP?066) in 2001, two in Serra Negra, Minas Gerais?MG (MG?012 and MG?016) in 2002, five in Caxias do Sul, Rio Grande do Sul?RS (RS?039, RS?045, RS?046, RS?047 and RS?058) in 2011 and one in Domingos Martins, Espírito Santo?ES (ES?148) in 2016. Disease incidence across all sampled fields ranged from 30% (in Domingos Martins?ES) to 90% in Sumaré?SP. Total DNA extraction was done by a modified CTAB method (Boiteux et al., 1999). Begomovirus infection was confirmed in all isolates by selective amplification of viral DNA?A segments using the primer pairs ?PAL1v1978 / PAR1c496? (Rojas et al., 1993) and ?BegomoAFor1? / ?BegomoARev1? (Ha et al., 2006), which produce two large and non?overlapping segments (≈1120 bp and ≈1205 bp, respectively). These PCR amplicons were initially characterized via direct Sanger dideoxy sequencing at CNPH. BLASTn analysis of the partial DNA?A genomes of these nine isolates indicated identity levels of 95?97% to three euphorbia yellow mosaic virus (EuYMV) reference isolates (= KY559532, JF756674, and KY559583) found infecting the weed Euphorbia heterophylla L. The entire DNA?A (2,609 nts = MN746971) and DNA?B (2,579 nts = MN746970) components of the MG?016 isolate were obtained via high?performance sequencing using Illumina HiSeq 2500 system (Macrogen Inc., South Korea). Sequences were assembled with the CLC Genomics Workbench program 10. Contigs were validated by BLASTx and BLASTn and compared to the ssDNA virus database at NCBI (www.ncbi.nlm.nih.gov). The fully?characterized MG?016 isolate displayed identity levels ranging from 97 to 99% to the EuYMV reference isolates as well as similar genomic features such as the conserved TATA box, nonanucleotide, and iterons (that were in agreement with a cognate nature of the DNA?A and DNA?B components). A partial sequence of the DNA?B genome was also obtained for the MG?012 isolate (MT7831942). The isolates MG?012 and MG?016 were found in mixed infections with tomato severe rugose virus (ToSRV) and tomato golden vein virus (TGVV), respectively. In addition, the complete DNA?A genomes of ES?148 (MN746972) and SP?066 (MN782438) were also obtained via a combination of primer walking and Sanger dideoxy sequencing, displaying 96?98% identity to EuYMV isolates. To our knowledge, this is the first report of multiple and independent events of natural infection of tomatoes by EuYMV isolates. Our results confirm the natural host status of tomatoes to EuYMV isolates as indicated in previous infectivity assays using biolistic inoculation (Barreto et al., 2013). The weed E. heterophylla is widely disseminated and very often present within tomato fields due to its higher levels of tolerance to the major herbicide (metribuzin) employed in this crop. Therefore, this weed may act as a persistent reservoir of tomato?infecting EuYMV isolates, which may allow the selection of viral populations potentially more adapted to this vegetable crop. MenosSevere yield losses induced by a complex of whitefly?transmitted Begomovirus species (family Geminiviridae) have been reported in tomatoes in Brazil (Reis et al. 2020). Nine isolates were obtained from tomato plants exhibiting begomovirus?like symptoms (viz. apical and interveinal chlorosis, yellow spots, and stunting) during independent field surveys: one isolate in Sumaré, São Paulo?SP State (isolate SP?066) in 2001, two in Serra Negra, Minas Gerais?MG (MG?012 and MG?016) in 2002, five in Caxias do Sul, Rio Grande do Sul?RS (RS?039, RS?045, RS?046, RS?047 and RS?058) in 2011 and one in Domingos Martins, Espírito Santo?ES (ES?148) in 2016. Disease incidence across all sampled fields ranged from 30% (in Domingos Martins?ES) to 90% in Sumaré?SP. Total DNA extraction was done by a modified CTAB method (Boiteux et al., 1999). Begomovirus infection was confirmed in all isolates by selective amplification of viral DNA?A segments using the primer pairs ?PAL1v1978 / PAR1c496? (Rojas et al., 1993) and ?BegomoAFor1? / ?BegomoARev1? (Ha et al., 2006), which produce two large and non?overlapping segments (≈1120 bp and ≈1205 bp, respectively). These PCR amplicons were initially characterized via direct Sanger dideoxy sequencing at CNPH. BLASTn analysis of the partial DNA?A genomes of these nine isolates indicated identity levels of 95?97% to three euphorbia yellow mosaic virus (EuYMV) reference isolates (= KY559532, JF756674, and KY559583) found infecting the weed Euphorbia ... Mostrar Tudo |
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Thesagro: |
Doença de Planta; Erva Daninha; Mosaico; Tomate. |
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Thesaurus NAL: |
Begomovirus. |
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Categoria do assunto: |
H Saúde e Patologia |
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URL: |
https://biblioteca.incaper.es.gov.br/digital/bitstream/123456789/4073/1/natural-infection-tomatoes-costa.pdf
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Marc: |
LEADER 04125naa a2200265 a 4500
001 1022277
005 2020-09-09
008 2020 bl uuuu u00u1 u #d
100 1 $aDUARTE, M. F.
245 $aNatural Infection of Tomatoes (Solanum lycopersicum) by Euphorbia yellow mosaic virus Isolates across Four Brazilian States.$h[electronic resource]
260 $c2020
520 $aSevere yield losses induced by a complex of whitefly?transmitted Begomovirus species (family Geminiviridae) have been reported in tomatoes in Brazil (Reis et al. 2020). Nine isolates were obtained from tomato plants exhibiting begomovirus?like symptoms (viz. apical and interveinal chlorosis, yellow spots, and stunting) during independent field surveys: one isolate in Sumaré, São Paulo?SP State (isolate SP?066) in 2001, two in Serra Negra, Minas Gerais?MG (MG?012 and MG?016) in 2002, five in Caxias do Sul, Rio Grande do Sul?RS (RS?039, RS?045, RS?046, RS?047 and RS?058) in 2011 and one in Domingos Martins, Espírito Santo?ES (ES?148) in 2016. Disease incidence across all sampled fields ranged from 30% (in Domingos Martins?ES) to 90% in Sumaré?SP. Total DNA extraction was done by a modified CTAB method (Boiteux et al., 1999). Begomovirus infection was confirmed in all isolates by selective amplification of viral DNA?A segments using the primer pairs ?PAL1v1978 / PAR1c496? (Rojas et al., 1993) and ?BegomoAFor1? / ?BegomoARev1? (Ha et al., 2006), which produce two large and non?overlapping segments (≈1120 bp and ≈1205 bp, respectively). These PCR amplicons were initially characterized via direct Sanger dideoxy sequencing at CNPH. BLASTn analysis of the partial DNA?A genomes of these nine isolates indicated identity levels of 95?97% to three euphorbia yellow mosaic virus (EuYMV) reference isolates (= KY559532, JF756674, and KY559583) found infecting the weed Euphorbia heterophylla L. The entire DNA?A (2,609 nts = MN746971) and DNA?B (2,579 nts = MN746970) components of the MG?016 isolate were obtained via high?performance sequencing using Illumina HiSeq 2500 system (Macrogen Inc., South Korea). Sequences were assembled with the CLC Genomics Workbench program 10. Contigs were validated by BLASTx and BLASTn and compared to the ssDNA virus database at NCBI (www.ncbi.nlm.nih.gov). The fully?characterized MG?016 isolate displayed identity levels ranging from 97 to 99% to the EuYMV reference isolates as well as similar genomic features such as the conserved TATA box, nonanucleotide, and iterons (that were in agreement with a cognate nature of the DNA?A and DNA?B components). A partial sequence of the DNA?B genome was also obtained for the MG?012 isolate (MT7831942). The isolates MG?012 and MG?016 were found in mixed infections with tomato severe rugose virus (ToSRV) and tomato golden vein virus (TGVV), respectively. In addition, the complete DNA?A genomes of ES?148 (MN746972) and SP?066 (MN782438) were also obtained via a combination of primer walking and Sanger dideoxy sequencing, displaying 96?98% identity to EuYMV isolates. To our knowledge, this is the first report of multiple and independent events of natural infection of tomatoes by EuYMV isolates. Our results confirm the natural host status of tomatoes to EuYMV isolates as indicated in previous infectivity assays using biolistic inoculation (Barreto et al., 2013). The weed E. heterophylla is widely disseminated and very often present within tomato fields due to its higher levels of tolerance to the major herbicide (metribuzin) employed in this crop. Therefore, this weed may act as a persistent reservoir of tomato?infecting EuYMV isolates, which may allow the selection of viral populations potentially more adapted to this vegetable crop.
650 $aBegomovirus
650 $aDoença de Planta
650 $aErva Daninha
650 $aMosaico
650 $aTomate
700 1 $aPEREIRA-CARVALHO, R. de C.
700 1 $aREIS, L. de N. A. dos
700 1 $aROJAS, M. J.
700 1 $aGILBERTSON, R.
700 1 $aCOSTA, H.
700 1 $aBOITEUX, L. S.
700 1 $aFONSECA, M. E. N.
773 $tPlant Disease, set. 2020.
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