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| 1. |  | BRANDANI, C. B.; ROCHA, J. H. T.; GODINHO, T. de O.; WENZEL, A. V.; GONÇALVES, J. L. de M. Compartimentalização de Al no solo em povoamentos florestais monoespecíficos e mistos de eucalyptus e acacia mangium. In: REUNIÃO BRASILEIRA DE FERTILIDADE DO SOLO E NUTRIÇÃO DE PLANTAS, 32.; REUNIÃO BRASILEIRA SOBRE MICORRIZAS, 16.; Simpósio Brasileiro de Microbiologia do Solo, 14.; XI REUNIÃO BRASILEIRA DE BIOLOGIA DO SOLO, 11., 2016, Goiânia. Rumo aos novos desafios: anais. Goiânia: UFG: UFMT: Instituto Federal Goiano: Embrapa: UnB, 2016. 161 p. FertBio 2016. Resumo.| Biblioteca(s): Biblioteca Rui Tendinha. |
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| 2. | | ROCHA, J. H. T.; BACKES, C.; BORELLI, k.; PRIETO, M. R.; SANTOS, A. J. M.; GODINHO, T. de O. Concentração de nutrientes e intensidade de cor verde em miniestacas de eucalipto. Biosci. J., Uberlândia, MG, v. 29, n. 6 , p. 1848-1856, Nov./Dez. 2013. 9 p.| Biblioteca(s): Biblioteca Rui Tendinha. |
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| 3. | | GODINHO, T. de O.; CALDEIRA, M. V. W.; ROCHA, J. H. T.; CALIMAN, J. P.; VIEIRA, M. Fertilidade do solo e nutrientes na serapilheira em fragmento de Floresta Estacional Semidecidual. Ecologia e Nutrição Florestal, Santa Maria-RS, v.1, n.3, p.97-109, set./dez., 2013 13 p.| Biblioteca(s): Biblioteca Rui Tendinha. |
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| 5. | | ROCHA, J. H. T.; BACKES, C.; BORELLI, K.; PRIETO, M. R.; SANTOS, A. J. M.; GODINHO, T. de O. Produtividade do minijardim e qualidade de miniestacas de um clone híbrido de Eucalyptus grandis x Eucalyptus urophylla (I-224) em função de doses de nitrogênio. Ciência Florestal, Santa Maria, v. 25, n. 2, p. 273-279, abr-jun, 2015.| Biblioteca(s): Biblioteca Rui Tendinha. |
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| 8. | | GODINHO, T. de O.; CALDEIRA, M. V. W.; CALIMAN, J. P.; PREZOTTI, L. C.; WATZLAWICK, L. F.; AZEVEDO, H. C. A. de.; ROCHA, J. H. T. Biomassa, macronutrientes e carbono Orgânico na serapilheira depositada em trecho de floresta Estacional Semidecidual Submontana, ES. Scientia Forestalis, v. 41, n. 97, p. 131-144, mar. 2013. 14p.| Biblioteca(s): Biblioteca Rui Tendinha. |
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| 9. | | ROCHA, J. H. T.; SANTOS, A. J. M.; DIOGO, F. A.; BACKES, C.; MELO, A. G. C. de.; BORELLI, K.; GODINHO, T. de O. Reflorestamento e recuperação de atributos químicos e físicos do solo. Floresta e Ambiente, v. 22, n. 3, p. 299-306, 2015 .| Biblioteca(s): Biblioteca Rui Tendinha. |
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| 10. | | ROCHA, J. H. T.; GONÇALVES, J. L. de M.; GAVA, J. L.; GODINHO, T. de O.; MELO, E. A. S. C.; BAZANI, J. H.; HUBNER, A.; ARTHUR JÚNIOR, J. C.; WICHERT, M. P. Forest residue maintenance increased the wood productivity of a Eucalyptus plantation over two short rotations. FOREST ECOLOGY AND MANAGEMENT, v. 379, p. 1-10, november 2016.| Biblioteca(s): Biblioteca Rui Tendinha. |
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Registro Completo |
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Biblioteca(s): |
Biblioteca Rui Tendinha. |
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Data corrente: |
13/01/2015 |
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Data da última atualização: |
13/01/2015 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
MARRACCINI, P.; VINECKY, F.; ALVES, G. S. C.; RAMOS, H. J. O.; ELBELT, S.; VIEIRA, N. G.; CARNEIRO, F. A.; SUJII, P. S.; ALEKCEVETCH, J. C.; SILVA, V. A.; DaMATTA, F. M.; FERRÃO, M. A. G.; LEROY, T.; POT, D.; VIEIRA, L. G. E.; SILVA, F. R. da; ANDRADE, A. C. |
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Afiliação: |
Maria Amélia Gava Ferrão, Incaper/Embrapa Café. |
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Título: |
Differentially expressed genes and proteins upon drought acclimation in tolerant and sensitive genotypes of Coffea canephora. |
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Ano de publicação: |
2012 |
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Fonte/Imprenta: |
Journal of Experimental Botany, Oxford v. 63, n. 11, p. 4191-4212, 2012. |
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Idioma: |
Inglês |
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Conteúdo: |
The aim of this study was to investigate the molecular mechanisms underlying drought acclimation in coffee plants by the identification of candidate genes (CGs) using different approaches. The first approach used the data generated during the Brazilian Coffee expressed sequence tag (EST) project to select 13 CGs by an in silico analysis (electronic northern). The second approach was based on screening macroarrays spotted with plasmid DNA (coffee ESTs) with separate hybridizations using leaf cDNA probes from drought-tolerant and susceptible clones of Coffea canephora var. Conilon, grown under different water regimes. This allowed the isolation of seven additional CGs. The third approach used two-dimensional gel electrophoresis to identify proteins displaying differential accumulation in leaves of drought-tolerant and susceptible clones of C. canephora. Six of them were characterized by MALDI-TOF-MS/MS (matrix-assisted laser desorption-time of flight-tandem mass spectrometry) and the corresponding proteins were identified. Finally, additional CGs were selected from the literature, and quantitative real-time polymerase chain reaction (qPCR) was performed to analyse the expression of all identified CGs. Altogether, >40 genes presenting differential gene expression during drought acclimation were identified, some of them showing different expression profiles between drought-tolerant and susceptible clones. Based on the obtained results, it can be concluded that factors involved a complex network of responses probably involving the abscisic signalling pathway and nitric oxide are major molecular determinants that might explain the better efficiency in controlling stomata closure and transpiration displayed by drought-tolerant clones of C. canephora. MenosThe aim of this study was to investigate the molecular mechanisms underlying drought acclimation in coffee plants by the identification of candidate genes (CGs) using different approaches. The first approach used the data generated during the Brazilian Coffee expressed sequence tag (EST) project to select 13 CGs by an in silico analysis (electronic northern). The second approach was based on screening macroarrays spotted with plasmid DNA (coffee ESTs) with separate hybridizations using leaf cDNA probes from drought-tolerant and susceptible clones of Coffea canephora var. Conilon, grown under different water regimes. This allowed the isolation of seven additional CGs. The third approach used two-dimensional gel electrophoresis to identify proteins displaying differential accumulation in leaves of drought-tolerant and susceptible clones of C. canephora. Six of them were characterized by MALDI-TOF-MS/MS (matrix-assisted laser desorption-time of flight-tandem mass spectrometry) and the corresponding proteins were identified. Finally, additional CGs were selected from the literature, and quantitative real-time polymerase chain reaction (qPCR) was performed to analyse the expression of all identified CGs. Altogether, >40 genes presenting differential gene expression during drought acclimation were identified, some of them showing different expression profiles between drought-tolerant and susceptible clones. Based on the obtained results, it can be concluded that factors involved a... Mostrar Tudo |
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Palavras-Chave: |
Candidate gene; Coffea canephora; Differential expression; Drought acclimation; Genética; Proteomic; Proteômica; Real time PCR. |
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Thesagro: |
Café; Coffea canephora; Genetics. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 03034naa a2200445 a 4500
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100 1 $aMARRACCINI, P.
245 $aDifferentially expressed genes and proteins upon drought acclimation in tolerant and sensitive genotypes of Coffea canephora.$h[electronic resource]
260 $c2012
520 $aThe aim of this study was to investigate the molecular mechanisms underlying drought acclimation in coffee plants by the identification of candidate genes (CGs) using different approaches. The first approach used the data generated during the Brazilian Coffee expressed sequence tag (EST) project to select 13 CGs by an in silico analysis (electronic northern). The second approach was based on screening macroarrays spotted with plasmid DNA (coffee ESTs) with separate hybridizations using leaf cDNA probes from drought-tolerant and susceptible clones of Coffea canephora var. Conilon, grown under different water regimes. This allowed the isolation of seven additional CGs. The third approach used two-dimensional gel electrophoresis to identify proteins displaying differential accumulation in leaves of drought-tolerant and susceptible clones of C. canephora. Six of them were characterized by MALDI-TOF-MS/MS (matrix-assisted laser desorption-time of flight-tandem mass spectrometry) and the corresponding proteins were identified. Finally, additional CGs were selected from the literature, and quantitative real-time polymerase chain reaction (qPCR) was performed to analyse the expression of all identified CGs. Altogether, >40 genes presenting differential gene expression during drought acclimation were identified, some of them showing different expression profiles between drought-tolerant and susceptible clones. Based on the obtained results, it can be concluded that factors involved a complex network of responses probably involving the abscisic signalling pathway and nitric oxide are major molecular determinants that might explain the better efficiency in controlling stomata closure and transpiration displayed by drought-tolerant clones of C. canephora.
650 $aCafé
650 $aCoffea canephora
650 $aGenetics
653 $aCandidate gene
653 $aCoffea canephora
653 $aDifferential expression
653 $aDrought acclimation
653 $aGenética
653 $aProteomic
653 $aProteômica
653 $aReal time PCR
700 1 $aVINECKY, F.
700 1 $aALVES, G. S. C.
700 1 $aRAMOS, H. J. O.
700 1 $aELBELT, S.
700 1 $aVIEIRA, N. G.
700 1 $aCARNEIRO, F. A.
700 1 $aSUJII, P. S.
700 1 $aALEKCEVETCH, J. C.
700 1 $aSILVA, V. A.
700 1 $aDaMATTA, F. M.
700 1 $aFERRÃO, M. A. G.
700 1 $aLEROY, T.
700 1 $aPOT, D.
700 1 $aVIEIRA, L. G. E.
700 1 $aSILVA, F. R. da
700 1 $aANDRADE, A. C.
773 $tJournal of Experimental Botany, Oxford$gv. 63, n. 11, p. 4191-4212, 2012.
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