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 | Acesso ao texto completo restrito à biblioteca da Biblioteca Rui Tendinha. Para informações adicionais entre em contato com biblioteca@incaper.es.gov.br. |
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Registro Completo |
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Biblioteca(s): |
Biblioteca Rui Tendinha. |
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Data corrente: |
06/07/2020 |
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Data da última atualização: |
06/07/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
MOURA, R. D.; CASTRO, L. A. M. de; CULIK, M. P.; FERNANDES, A. A. R.; FERNANDES, P. M. B.; VENTURA, J. A. |
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Afiliação: |
Raíssa Debacker Moura; Luiza Adami Monteiro de Castro; Mark Paul Culik, CNPq/Incaper; Antônio Alberto Ribeiro Fernandes; Patricia Machado Bueno Fernandes; Jose Aires Ventura, Incaper. |
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Título: |
Culture medium for improved production of conidia for identification and systematic studies of Fusarium pathogens. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Journal of Microbiological Methods, v. 173, June 2020. |
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DOI: |
10.1016/j.mimet.2020.105915 |
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Idioma: |
Inglês |
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Conteúdo: |
Fusarium guttiforme and Fusarium ananatum are the etiological agents of fusariosis and fruitlet core rot in pineapple, espectively, producing mycotoxins that are harmful to the health of consumers. These two fungi are morphologically similar and difficulty in obtaining macroconidia of the species limits their identification. Different types of media are available for the culture of these pathogens, but not all of them favor F. ananatum
and F. guttiforme macroconidia production. Therefore, the objective of this study was to develop a simple culture medium to improve rapid macro- and microconidia formation in both F. guttiforme and F. ananatum to facilitate taxonomic, pathogenicity and mycotoxin studies. In vitro analysis showed that basal medium with carboxymethyl cellulose (CMC) was better than other media tested with the highest macroconidia production at 7 days of incubation. The highest production of microconidia was with synthetic nutrient medium (SN) at 7 days. F. ananatum produced a relatively high number of microconidia with one septum in comparison to F. guttiforme when cultured in CMC, which suggests an additional character useful for Fusarium taxonomy. CMC medium may serve as an improved alternative to culture media currently used in Fusarium research and contribute to further knowledge of the taxonomy and mycotoxins of Fusarium species. |
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Palavras-Chave: |
Abacaxi. |
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Thesagro: |
Fusariose; Fusarium; Patogenicidade; Taxonomia. |
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Thesaurus NAL: |
Carboxymethyl cellulose; Sporulation; Taxonomy. |
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Categoria do assunto: |
H Saúde e Patologia |
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Marc: |
LEADER 02225naa a2200289 a 4500
001 1022238
005 2020-07-06
008 2020 bl uuuu u00u1 u #d
024 7 $a10.1016/j.mimet.2020.105915$2DOI
100 1 $aMOURA, R. D.
245 $aCulture medium for improved production of conidia for identification and systematic studies of Fusarium pathogens.$h[electronic resource]
260 $c2020
520 $aFusarium guttiforme and Fusarium ananatum are the etiological agents of fusariosis and fruitlet core rot in pineapple, espectively, producing mycotoxins that are harmful to the health of consumers. These two fungi are morphologically similar and difficulty in obtaining macroconidia of the species limits their identification. Different types of media are available for the culture of these pathogens, but not all of them favor F. ananatum and F. guttiforme macroconidia production. Therefore, the objective of this study was to develop a simple culture medium to improve rapid macro- and microconidia formation in both F. guttiforme and F. ananatum to facilitate taxonomic, pathogenicity and mycotoxin studies. In vitro analysis showed that basal medium with carboxymethyl cellulose (CMC) was better than other media tested with the highest macroconidia production at 7 days of incubation. The highest production of microconidia was with synthetic nutrient medium (SN) at 7 days. F. ananatum produced a relatively high number of microconidia with one septum in comparison to F. guttiforme when cultured in CMC, which suggests an additional character useful for Fusarium taxonomy. CMC medium may serve as an improved alternative to culture media currently used in Fusarium research and contribute to further knowledge of the taxonomy and mycotoxins of Fusarium species.
650 $aCarboxymethyl cellulose
650 $aSporulation
650 $aTaxonomy
650 $aFusariose
650 $aFusarium
650 $aPatogenicidade
650 $aTaxonomia
653 $aAbacaxi
700 1 $aCASTRO, L. A. M. de
700 1 $aCULIK, M. P.
700 1 $aFERNANDES, A. A. R.
700 1 $aFERNANDES, P. M. B.
700 1 $aVENTURA, J. A.
773 $tJournal of Microbiological Methods$gv. 173, June 2020.
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Registro original: |
Biblioteca Rui Tendinha (BRT) |
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Tipo/Formato |
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| Acesso ao texto completo restrito à biblioteca da Biblioteca Rui Tendinha. Para informações adicionais entre em contato com biblioteca@incaper.es.gov.br. |
|
Registro Completo |
|
Biblioteca(s): |
Biblioteca Rui Tendinha. |
|
Data corrente: |
06/07/2021 |
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Data da última atualização: |
25/08/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
MOURA, R. D.; CARVALHO, L. M.; SPAGNOL, B. A. A.; CARNEIRO, T.; COSTA, A. C. T.; QUADROS, O. de F.; VENTURA, J. A.; BIASI, R. S.; FERNANDES, A. A. R.; FERNANDES, P. M. B. |
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Afiliação: |
Raissa D. Moura; Lauanda M. Carvalho; Brígida A. A. Spagnol; Tarcio Carneiro; Ane Catarine Tosi Costa; Oeber de F. Quadros; Jose Aires Ventura, Incaper; Ronaldo S. de Biasi; A. Alberto R. Fernandes; Patricia M. B. Fernandes. |
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Título: |
Difference between the cell wall roughnesses of mothers and daughters of Saccharomyces cerevisiae subjected to high pressure stress. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
Micron, v. 147, p. 1-5, 2021. |
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Idioma: |
Inglês |
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Conteúdo: |
High hydrostatic pressure (HHP) stress generates cellular responses similar to those to other stresses that yeasts endure in fermentation tanks. Structural and spatial compaction of molecules, as well as weakening and stretching of plasma membranes and cell walls, are often observed and have a significant influence on the fermentative process. Atomic force microscopy (AFM) yields accurate data on the morphological characteristics of yeast cell walls, providing important insights for the development of more productive yeast strains. Saccharomyces cerevisiae cell wall assessment using AFM in the intermittent contact reading mode using a silicon cantilever, before and after application of a pressure of 100 MPa for 30 min, demonstrated that mother and daughter cells have different responses. Daughter cells were more sensitive to the effects of HHP, presenting lower average Ra (arithmetic roughness), Rz (ten-point average roughness), and Rq (root-mean-square roughness) after exposure to high pressure. Better adaptation to stress in mother cells leads to higher cell wall resistance and, therefore, to better protection. |
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Palavras-Chave: |
Força atômica; Hydrostatic pressure; Piezostress. |
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Thesagro: |
Pressão Hidrostática. |
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Thesaurus NAL: |
Atomic force microscopy; Saccharomyces cerevisiae var. cerevisiae. |
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Categoria do assunto: |
H Saúde e Patologia |
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Marc: |
LEADER 02063naa a2200301 a 4500
001 1023196
005 2022-08-25
008 2021 bl uuuu u00u1 u #d
100 1 $aMOURA, R. D.
245 $aDifference between the cell wall roughnesses of mothers and daughters of Saccharomyces cerevisiae subjected to high pressure stress.$h[electronic resource]
260 $c2021
520 $aHigh hydrostatic pressure (HHP) stress generates cellular responses similar to those to other stresses that yeasts endure in fermentation tanks. Structural and spatial compaction of molecules, as well as weakening and stretching of plasma membranes and cell walls, are often observed and have a significant influence on the fermentative process. Atomic force microscopy (AFM) yields accurate data on the morphological characteristics of yeast cell walls, providing important insights for the development of more productive yeast strains. Saccharomyces cerevisiae cell wall assessment using AFM in the intermittent contact reading mode using a silicon cantilever, before and after application of a pressure of 100 MPa for 30 min, demonstrated that mother and daughter cells have different responses. Daughter cells were more sensitive to the effects of HHP, presenting lower average Ra (arithmetic roughness), Rz (ten-point average roughness), and Rq (root-mean-square roughness) after exposure to high pressure. Better adaptation to stress in mother cells leads to higher cell wall resistance and, therefore, to better protection.
650 $aAtomic force microscopy
650 $aSaccharomyces cerevisiae var. cerevisiae
650 $aPressão Hidrostática
653 $aForça atômica
653 $aHydrostatic pressure
653 $aPiezostress
700 1 $aCARVALHO, L. M.
700 1 $aSPAGNOL, B. A. A.
700 1 $aCARNEIRO, T.
700 1 $aCOSTA, A. C. T.
700 1 $aQUADROS, O. de F.
700 1 $aVENTURA, J. A.
700 1 $aBIASI, R. S.
700 1 $aFERNANDES, A. A. R.
700 1 $aFERNANDES, P. M. B.
773 $tMicron$gv. 147, p. 1-5, 2021.
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