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Biblioteca(s): |
Biblioteca Rui Tendinha. |
Data corrente: |
13/01/2015 |
Data da última atualização: |
13/01/2015 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
MARRACCINI, P.; VINECKY, F.; ALVES, G. S. C.; RAMOS, H. J. O.; ELBELT, S.; VIEIRA, N. G.; CARNEIRO, F. A.; SUJII, P. S.; ALEKCEVETCH, J. C.; SILVA, V. A.; DaMATTA, F. M.; FERRÃO, M. A. G.; LEROY, T.; POT, D.; VIEIRA, L. G. E.; SILVA, F. R. da; ANDRADE, A. C. |
Afiliação: |
Maria Amélia Gava Ferrão, Incaper/Embrapa Café. |
Título: |
Differentially expressed genes and proteins upon drought acclimation in tolerant and sensitive genotypes of Coffea canephora. |
Ano de publicação: |
2012 |
Fonte/Imprenta: |
Journal of Experimental Botany, Oxford v. 63, n. 11, p. 4191-4212, 2012. |
Idioma: |
Inglês |
Conteúdo: |
The aim of this study was to investigate the molecular mechanisms underlying drought acclimation in coffee plants by the identification of candidate genes (CGs) using different approaches. The first approach used the data generated during the Brazilian Coffee expressed sequence tag (EST) project to select 13 CGs by an in silico analysis (electronic northern). The second approach was based on screening macroarrays spotted with plasmid DNA (coffee ESTs) with separate hybridizations using leaf cDNA probes from drought-tolerant and susceptible clones of Coffea canephora var. Conilon, grown under different water regimes. This allowed the isolation of seven additional CGs. The third approach used two-dimensional gel electrophoresis to identify proteins displaying differential accumulation in leaves of drought-tolerant and susceptible clones of C. canephora. Six of them were characterized by MALDI-TOF-MS/MS (matrix-assisted laser desorption-time of flight-tandem mass spectrometry) and the corresponding proteins were identified. Finally, additional CGs were selected from the literature, and quantitative real-time polymerase chain reaction (qPCR) was performed to analyse the expression of all identified CGs. Altogether, >40 genes presenting differential gene expression during drought acclimation were identified, some of them showing different expression profiles between drought-tolerant and susceptible clones. Based on the obtained results, it can be concluded that factors involved a complex network of responses probably involving the abscisic signalling pathway and nitric oxide are major molecular determinants that might explain the better efficiency in controlling stomata closure and transpiration displayed by drought-tolerant clones of C. canephora. MenosThe aim of this study was to investigate the molecular mechanisms underlying drought acclimation in coffee plants by the identification of candidate genes (CGs) using different approaches. The first approach used the data generated during the Brazilian Coffee expressed sequence tag (EST) project to select 13 CGs by an in silico analysis (electronic northern). The second approach was based on screening macroarrays spotted with plasmid DNA (coffee ESTs) with separate hybridizations using leaf cDNA probes from drought-tolerant and susceptible clones of Coffea canephora var. Conilon, grown under different water regimes. This allowed the isolation of seven additional CGs. The third approach used two-dimensional gel electrophoresis to identify proteins displaying differential accumulation in leaves of drought-tolerant and susceptible clones of C. canephora. Six of them were characterized by MALDI-TOF-MS/MS (matrix-assisted laser desorption-time of flight-tandem mass spectrometry) and the corresponding proteins were identified. Finally, additional CGs were selected from the literature, and quantitative real-time polymerase chain reaction (qPCR) was performed to analyse the expression of all identified CGs. Altogether, >40 genes presenting differential gene expression during drought acclimation were identified, some of them showing different expression profiles between drought-tolerant and susceptible clones. Based on the obtained results, it can be concluded that factors involved a... Mostrar Tudo |
Palavras-Chave: |
Candidate gene; Coffea canephora; Differential expression; Drought acclimation; Genética; Proteomic; Proteômica; Real time PCR. |
Thesagro: |
Café; Coffea canephora; Genetics. |
Categoria do assunto: |
-- |
Marc: |
LEADER 03034naa a2200445 a 4500 001 1004907 005 2015-01-13 008 2012 bl uuuu u00u1 u #d 100 1 $aMARRACCINI, P. 245 $aDifferentially expressed genes and proteins upon drought acclimation in tolerant and sensitive genotypes of Coffea canephora.$h[electronic resource] 260 $c2012 520 $aThe aim of this study was to investigate the molecular mechanisms underlying drought acclimation in coffee plants by the identification of candidate genes (CGs) using different approaches. The first approach used the data generated during the Brazilian Coffee expressed sequence tag (EST) project to select 13 CGs by an in silico analysis (electronic northern). The second approach was based on screening macroarrays spotted with plasmid DNA (coffee ESTs) with separate hybridizations using leaf cDNA probes from drought-tolerant and susceptible clones of Coffea canephora var. Conilon, grown under different water regimes. This allowed the isolation of seven additional CGs. The third approach used two-dimensional gel electrophoresis to identify proteins displaying differential accumulation in leaves of drought-tolerant and susceptible clones of C. canephora. Six of them were characterized by MALDI-TOF-MS/MS (matrix-assisted laser desorption-time of flight-tandem mass spectrometry) and the corresponding proteins were identified. Finally, additional CGs were selected from the literature, and quantitative real-time polymerase chain reaction (qPCR) was performed to analyse the expression of all identified CGs. Altogether, >40 genes presenting differential gene expression during drought acclimation were identified, some of them showing different expression profiles between drought-tolerant and susceptible clones. Based on the obtained results, it can be concluded that factors involved a complex network of responses probably involving the abscisic signalling pathway and nitric oxide are major molecular determinants that might explain the better efficiency in controlling stomata closure and transpiration displayed by drought-tolerant clones of C. canephora. 650 $aCafé 650 $aCoffea canephora 650 $aGenetics 653 $aCandidate gene 653 $aCoffea canephora 653 $aDifferential expression 653 $aDrought acclimation 653 $aGenética 653 $aProteomic 653 $aProteômica 653 $aReal time PCR 700 1 $aVINECKY, F. 700 1 $aALVES, G. S. C. 700 1 $aRAMOS, H. J. O. 700 1 $aELBELT, S. 700 1 $aVIEIRA, N. G. 700 1 $aCARNEIRO, F. A. 700 1 $aSUJII, P. S. 700 1 $aALEKCEVETCH, J. C. 700 1 $aSILVA, V. A. 700 1 $aDaMATTA, F. M. 700 1 $aFERRÃO, M. A. G. 700 1 $aLEROY, T. 700 1 $aPOT, D. 700 1 $aVIEIRA, L. G. E. 700 1 $aSILVA, F. R. da 700 1 $aANDRADE, A. C. 773 $tJournal of Experimental Botany, Oxford$gv. 63, n. 11, p. 4191-4212, 2012.
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Biblioteca Rui Tendinha (BRT) |
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Biblioteca(s): |
Biblioteca Rui Tendinha. |
Data corrente: |
03/03/2016 |
Data da última atualização: |
10/10/2019 |
Autoria: |
KUGIZAKI, Y.; MUNIZ, J. M. |
Afiliação: |
Yoichi Kugizaki, EMCAPA; Jorge Machado Muniz, EMCAPA. |
Título: |
Limão: variação estacional de preços no Estado do Espírito Santo. |
Ano de publicação: |
1985 |
Fonte/Imprenta: |
Vitória, ES : EMCAPA, 1985. |
Páginas: |
8 p. |
Série: |
(EMCAPA. Documentos, 23). |
ISSN: |
0101-8949 |
Idioma: |
Português |
Notas: |
Errata: Na página 4 onde está o termo Tomate, lê-se Limão. |
Conteúdo: |
No presente trabalho, foram analisadas as variacoes estacionais de precos do limao 'Tahity' no mercado atacadista da CEASA-ES, no periodo de 1978. Este mercado e abastecido nao somente pela producao estadual, mas, tambem, pela importacao de outros Estados, tais como Rio de Janeiro, Sao Paulo, Sergipe e Bahia. |
Palavras-Chave: |
Brasil; Brazil; Citros; Espirito Santo; Lemon; Limao tahity; Preco; Price; Variacao estacional; Variation. |
Categoria do assunto: |
-- |
URL: |
http://biblioteca.incaper.es.gov.br/digital/bitstream/item/1594/1/BRT-doc23-limaovariacaoestacionaldeprecos-Emcapa.pdf
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Marc: |
LEADER 01063nam a2200289 a 4500 001 1010066 005 2019-10-10 008 1985 bl uuuu u0uu1 u #d 022 $a0101-8949 100 1 $aKUGIZAKI, Y. 245 $aLimão$bvariação estacional de preços no Estado do Espírito Santo. 260 $aVitória, ES : EMCAPA$c1985 300 $a8 p. 490 $a(EMCAPA. Documentos, 23). 500 $aErrata: Na página 4 onde está o termo Tomate, lê-se Limão. 520 $aNo presente trabalho, foram analisadas as variacoes estacionais de precos do limao 'Tahity' no mercado atacadista da CEASA-ES, no periodo de 1978. Este mercado e abastecido nao somente pela producao estadual, mas, tambem, pela importacao de outros Estados, tais como Rio de Janeiro, Sao Paulo, Sergipe e Bahia. 653 $aBrasil 653 $aBrazil 653 $aCitros 653 $aEspirito Santo 653 $aLemon 653 $aLimao tahity 653 $aPreco 653 $aPrice 653 $aVariacao estacional 653 $aVariation 700 1 $aMUNIZ, J. M.
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