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Registro Completo |
Biblioteca(s): |
Biblioteca Rui Tendinha. |
Data corrente: |
11/04/2024 |
Data da última atualização: |
11/04/2024 |
Autoria: |
FERRÃO, M. A. G.; FONSECA, A. F. A. da.; FERRÃO, R. G.; MARRACCINI, P.; POT, D.; LEROY, T.; JR. BLOCH, C.; ANDRADE, A. C. |
Afiliação: |
Maria Amélia Gava Ferrão, Incaper/Embrapa Café; Aymbiré Francisco Almeida da Fonseca, Incaper/Embrapa Café; Romário Gava Ferrão, Incaper; Pierre Marraccini; David Pot; Thierry Leroy; Carlos Jr. Bloch; Alan C. Andrade. |
Título: |
Study of biological mechanims involved in drought tolerance in coffee plants. |
Ano de publicação: |
2009 |
Fonte/Imprenta: |
In: 22nd International Conference on Coffee Science, ASIC 2008, Campinas, SP, Brazil, 14-19 September, 2008. Association Scientifique Internationale du Café (ASIC), 2009. p. 874-881. |
Páginas: |
p. 874-881 |
Idioma: |
Português |
Conteúdo: |
The principal aim of this study was to investigate the molecular mechanisms underlying the response to drought stress in coffee plants by different approaches. In order to identify candidate genes involved in controlling drought tolerance in coffee plants, different strategies were followed in our laboratories. The first used the nucleic data generated by the Brazilian Coffee EST project to identify candidate genes (CG) by in silico analysis (electronic Northern-blot). Differential expression of these CG was verified in leaves and roots from drought-tolerant and susceptible clones of C. canephora var. Conilon by Northern-blot and quantitative PCR experiments. The second was based on the screening of macroarray membranes spotted with coffee ESTs which were hybridized separately with leaf cDNA probes of the same clones. Finally, 2D gel electrophoresis was also performed to selected proteins presenting differential accumulation in leaves of the same clones. These proteins were analyzed by MALDI-TOF-MS/MS leading to the identification of a new set of CG. Results concerning the identification of CG by these different approaches are presented and discussed. |
Thesagro: |
Coffea Canephora; DNA; Gene. |
Categoria do assunto: |
-- |
URL: |
https://biblioteca.incaper.es.gov.br/digital/bitstream/item/4668/1/Study-of-Biological-Mechanims-Involved-in-Drought-Tolerance-in-Coffee-Plants.pdf
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Marc: |
LEADER 01994naa a2200253 a 4500 001 1025586 005 2024-04-11 008 2009 bl uuuu u00u1 u #d 100 1 $aFERRÃO, M. A. G. 245 $aStudy of biological mechanims involved in drought tolerance in coffee plants.$h[electronic resource] 260 $c2009 300 $ap. 874-881 520 $aThe principal aim of this study was to investigate the molecular mechanisms underlying the response to drought stress in coffee plants by different approaches. In order to identify candidate genes involved in controlling drought tolerance in coffee plants, different strategies were followed in our laboratories. The first used the nucleic data generated by the Brazilian Coffee EST project to identify candidate genes (CG) by in silico analysis (electronic Northern-blot). Differential expression of these CG was verified in leaves and roots from drought-tolerant and susceptible clones of C. canephora var. Conilon by Northern-blot and quantitative PCR experiments. The second was based on the screening of macroarray membranes spotted with coffee ESTs which were hybridized separately with leaf cDNA probes of the same clones. Finally, 2D gel electrophoresis was also performed to selected proteins presenting differential accumulation in leaves of the same clones. These proteins were analyzed by MALDI-TOF-MS/MS leading to the identification of a new set of CG. Results concerning the identification of CG by these different approaches are presented and discussed. 650 $aCoffea Canephora 650 $aDNA 650 $aGene 700 1 $aFONSECA, A. F. A. da. 700 1 $aFERRÃO, R. G. 700 1 $aMARRACCINI, P. 700 1 $aPOT, D. 700 1 $aLEROY, T. 700 1 $aJR. BLOCH, C. 700 1 $aANDRADE, A. C. 773 $tIn: 22nd International Conference on Coffee Science, ASIC 2008, Campinas, SP, Brazil, 14-19 September, 2008. Association Scientifique Internationale du Café (ASIC), 2009. p. 874-881.
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Biblioteca Rui Tendinha (BRT) |
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Registro Completo |
Biblioteca(s): |
Biblioteca Rui Tendinha. |
Data corrente: |
26/08/2015 |
Data da última atualização: |
26/08/2015 |
Tipo da produção científica: |
Publicação em Anais de Congresso |
Autoria: |
CARNIELLI, L.; FERNANDES, P. M. B.; FERNANDES, A. A. R.; VENTURA, J. A. |
Afiliação: |
Jose Aires Ventura, Incaper. |
Título: |
Diagnóstico molecular de Fusarium guttiforme com PCR em tempo real. |
Ano de publicação: |
2015 |
Fonte/Imprenta: |
In: CONGRESSO BRASILEIRO DE FITOPATOLOGIA, 48.; CONGRESSO BRASILEIRO DE PATOLOGIA PÓS-COLHEITA, 2., 2015, São Pedro, SP. Fitopatologia de precisão: fronteiras da ciência: anais. São Pedro, SP: Sociedade Brasileira de Fitopatologia, 2015. |
Idioma: |
Português |
Palavras-Chave: |
Abacaxi; Fusariose; Fusarium guttiforme. |
Categoria do assunto: |
-- |
URL: |
http://biblioteca.incaper.es.gov.br/digital/bitstream/item/994/1/Resumo-389-1-Fusarium-guttiforme-Ventura.pdf
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Marc: |
LEADER 00707nam a2200169 a 4500 001 1007621 005 2015-08-26 008 2015 bl uuuu u01u1 u #d 100 1 $aCARNIELLI, L. 245 $aDiagnóstico molecular de Fusarium guttiforme com PCR em tempo real.$h[electronic resource] 260 $aIn: CONGRESSO BRASILEIRO DE FITOPATOLOGIA, 48.; CONGRESSO BRASILEIRO DE PATOLOGIA PÓS-COLHEITA, 2., 2015, São Pedro, SP. Fitopatologia de precisão: fronteiras da ciência: anais. São Pedro, SP: Sociedade Brasileira de Fitopatologia$c2015 653 $aAbacaxi 653 $aFusariose 653 $aFusarium guttiforme 700 1 $aFERNANDES, P. M. B. 700 1 $aFERNANDES, A. A. R. 700 1 $aVENTURA, J. A.
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