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| Registros recuperados : 28 | |
| 10. |  | ARLEU, R. J. A.; FURTADO, M. J.; FANTON, C. J. Flutuação populacional do Erinnyis ello (L., 1758) em mandioca, no município de Linhares, Espírito Santo. COMUNICADO TÉCNICO, n. 45, p. 1-6, jun. 1986. (EMCAPA. Comunicado Técnico, 45).| Biblioteca(s): Biblioteca Rui Tendinha. |
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| 12. | | ARLEU, R. J. A.; GOMES, J. A.; NOBREGA, A. C. Nível de controle para broca da bananeira - Cosmopolites sordidus (germ., 1824), no Espírito Santo. In: INTERNATIONAL SYMPOSIUM ON TROPICAL FRUITS, 1., 1993, Vitória, ES. Program and abstracts. Vitória : EMCAPA, p. 16, 1993. (EMCAPA. Documentos, 79).| Biblioteca(s): Biblioteca Rui Tendinha. |
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| 15. | | FORNAZIER, M. J.; MARTINS, D. dos S.; ARLEU, R. J. A.; BENASSI, V. L. R. M.; DE MUNER, L. H. Caracterização da infestação de campo da broca-do-café, em café Arábica e Conilon, no Estado do Espírito Santo. IN: SIMPÓSIO DE PESQUISA DOS CAFÉS DO BRASIL, 1., 2000, Poços de Caldas. Resumos Expandidos. Brasília, DF: Embrapa Café/MINASPLAN, 2000. p. 1157-1160.| Biblioteca(s): Biblioteca Rui Tendinha. |
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| 18. | | ARLEU, R. J. A.; PISSARRA, T. B.; MUNIZ, J. M.; SOUZA, I. A. de.; BRAVIM, A. J. B. Controle quimico do acaro do abacaxizeiro (Dolichotetranychus floridanus Banks, 1900). INDICAÇÃO EMCAPA, Cariacica, n. 2, p. 1-6, 1978. 07p. (EMCAPA. Indicação EMCAPA, 02/78).| Biblioteca(s): Biblioteca Rui Tendinha. |
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| 20. | | FORNAZIER, M. J.; MARTINS, D. dos S.; DE MUNER, L. H.; BENASSI, V. L. R. M.; ARLEU, R. J. A. Infestação de campo da broca-do-café, em café conilon, no Estado do Espírito Santo - Safra 2000/2001. In: SIMPÓSIO DE PESQUISA DOS CAFÉS DO BRASIL, 2., 2001, Vitória, ES. Trabalhos apresentados... Brasília, DF : Embrapa Café, 2001. 8p.| Biblioteca(s): Biblioteca Rui Tendinha. |
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Registro Completo |
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Biblioteca(s): |
Biblioteca Rui Tendinha. |
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Data corrente: |
29/07/2019 |
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Data da última atualização: |
29/07/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
ANTUNES, T. F. S.; AMARAL, R. J. V.; VENTURA, J. A.; GODINHO, M. T.; AMARAL, J. G.; SOUZA, F. O.; ZERBINI, P. A.; FERNANDES, P. M. B. |
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Afiliação: |
Tathiana Ferreira Sá Antunes, UFES; Raquel J. Vionette Amaral, UFES; Jose Aires Ventura, Incaper; Marcio Tadeu Godinho, UFV; Josiane G. Amaral, UFV; Flávia O. Souza, UFV; Poliane Alfenas Zerbini, UFV; Patricia Machado Bueno Fernandes, UFES. |
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Título: |
The dsRNA Virus Papaya Meleira Virus and an ssRNA Virus Are Associated with Papaya Sticky Disease. |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
PLoS ONE, v. 11, n. 5, e0155240. |
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Idioma: |
Português |
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Conteúdo: |
Papaya sticky disease, or ?meleira?, is one of the major diseases of papaya in Brazil and Mexico, capable of causing complete crop loss. The causal agent of sticky disease was identified as an isometric virus with a double stranded RNA (dsRNA) genome, named papaya meleira virus (PMeV). In the present study, PMeV dsRNA and a second RNA band of approximately 4.5 kb, both isolated from latex of papaya plants with severe symptoms of sticky disease, were deep-sequenced. The nearly complete sequence obtained for PMeV dsRNA is 8,814 nucleotides long and contains two putative ORFs; the predicted ORF1 and
ORF2 display similarity to capsid proteins and RdRp's, respectively, from mycoviruses tentatively classified in the family Totiviridae. The sequence obtained for the second RNA is 4,515 nucleotides long and contains two putative ORFs. The predicted ORFs 1 and 2 display 48% and 73% sequence identity, respectively, with the corresponding proteins of papaya virus Q, an umbravirus recently described infecting papaya in Ecuador. Viral purification in a sucrose gradient allowed separation of particles containing each RNA. Mass spectrometry analysis indicated that both PMeV and the second RNA virus (named papaya
meleira virus 2, PMeV2) were encapsidated in particles formed by the protein encoded by PMeV ORF1. The presence of both PMeV and PMeV2 was confirmed in field plants showing typical symptoms of sticky disease. Interestingly, PMeV was detected alone in asymptomatic plants. Together, our results indicate that sticky disease is associated with double infection by PMeV and PMeV2. MenosPapaya sticky disease, or ?meleira?, is one of the major diseases of papaya in Brazil and Mexico, capable of causing complete crop loss. The causal agent of sticky disease was identified as an isometric virus with a double stranded RNA (dsRNA) genome, named papaya meleira virus (PMeV). In the present study, PMeV dsRNA and a second RNA band of approximately 4.5 kb, both isolated from latex of papaya plants with severe symptoms of sticky disease, were deep-sequenced. The nearly complete sequence obtained for PMeV dsRNA is 8,814 nucleotides long and contains two putative ORFs; the predicted ORF1 and
ORF2 display similarity to capsid proteins and RdRp's, respectively, from mycoviruses tentatively classified in the family Totiviridae. The sequence obtained for the second RNA is 4,515 nucleotides long and contains two putative ORFs. The predicted ORFs 1 and 2 display 48% and 73% sequence identity, respectively, with the corresponding proteins of papaya virus Q, an umbravirus recently described infecting papaya in Ecuador. Viral purification in a sucrose gradient allowed separation of particles containing each RNA. Mass spectrometry analysis indicated that both PMeV and the second RNA virus (named papaya
meleira virus 2, PMeV2) were encapsidated in particles formed by the protein encoded by PMeV ORF1. The presence of both PMeV and PMeV2 was confirmed in field plants showing typical symptoms of sticky disease. Interestingly, PMeV was detected alone in asymptomatic plants. Together, ... Mostrar Tudo |
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Palavras-Chave: |
Mamão; Mamoeiro; Meleira; Papaya Meleira Virus. |
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Categoria do assunto: |
G Melhoramento Genético |
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URL: |
https://biblioteca.incaper.es.gov.br/digital/bitstream/123456789/3645/1/the-dsrna-papaya-ventura.PDF
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Marc: |
LEADER 02305naa a2200253 a 4500
001 1021491
005 2019-07-29
008 2016 bl uuuu u00u1 u #d
100 1 $aANTUNES, T. F. S.
245 $aThe dsRNA Virus Papaya Meleira Virus and an ssRNA Virus Are Associated with Papaya Sticky Disease.$h[electronic resource]
260 $c2016
520 $aPapaya sticky disease, or ?meleira?, is one of the major diseases of papaya in Brazil and Mexico, capable of causing complete crop loss. The causal agent of sticky disease was identified as an isometric virus with a double stranded RNA (dsRNA) genome, named papaya meleira virus (PMeV). In the present study, PMeV dsRNA and a second RNA band of approximately 4.5 kb, both isolated from latex of papaya plants with severe symptoms of sticky disease, were deep-sequenced. The nearly complete sequence obtained for PMeV dsRNA is 8,814 nucleotides long and contains two putative ORFs; the predicted ORF1 and ORF2 display similarity to capsid proteins and RdRp's, respectively, from mycoviruses tentatively classified in the family Totiviridae. The sequence obtained for the second RNA is 4,515 nucleotides long and contains two putative ORFs. The predicted ORFs 1 and 2 display 48% and 73% sequence identity, respectively, with the corresponding proteins of papaya virus Q, an umbravirus recently described infecting papaya in Ecuador. Viral purification in a sucrose gradient allowed separation of particles containing each RNA. Mass spectrometry analysis indicated that both PMeV and the second RNA virus (named papaya meleira virus 2, PMeV2) were encapsidated in particles formed by the protein encoded by PMeV ORF1. The presence of both PMeV and PMeV2 was confirmed in field plants showing typical symptoms of sticky disease. Interestingly, PMeV was detected alone in asymptomatic plants. Together, our results indicate that sticky disease is associated with double infection by PMeV and PMeV2.
653 $aMamão
653 $aMamoeiro
653 $aMeleira
653 $aPapaya Meleira Virus
700 1 $aAMARAL, R. J. V.
700 1 $aVENTURA, J. A.
700 1 $aGODINHO, M. T.
700 1 $aAMARAL, J. G.
700 1 $aSOUZA, F. O.
700 1 $aZERBINI, P. A.
700 1 $aFERNANDES, P. M. B.
773 $tPLoS ONE$gv. 11, n. 5, e0155240.
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