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Registro Completo |
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Biblioteca(s): |
Biblioteca Rui Tendinha. |
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Data corrente: |
17/12/2015 |
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Data da última atualização: |
17/12/2015 |
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Autoria: |
BRITO, M. A. V. P. e.; BRITO, J. R. F. |
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Título: |
Diagnóstico microbiológico da mastite. |
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Ano de publicação: |
1999 |
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Fonte/Imprenta: |
Juiz de Fora : Embrapa Gado de Leite, 1999. |
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Páginas: |
26 p. |
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Série: |
(Embrapa Gado de Leite. Circular Tecnica, 55). |
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Idioma: |
Português |
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Conteúdo: |
Exames de casos clínicos; Cultura do leite para avaliação do rebanho; Cultura do leite total do rebanho; Coleta das amostras de leite; Identificação dos patógenos da mastite; Cocos gram-positivos, catalase positivos; Cocos gram-positivos, catalase negativos; Bactérias gram-negativas; Bastonetes gram-positivos; Outros micro organismos; Amostras contaminadas; Antibiograma; Procedimentos para a coleta de leite para exame microbiológicos; Material necessário; Quando coletar as amostras; Preparação das tetas; Coleta das amostras; Manutenção das amostras; Informações sobre as amostras. |
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Palavras-Chave: |
Analise de laboratorio; Animal diseases; Bacteria; Bacterioses; Bovine mastitis; Bovino; Bovino de leite; Bovino de Leite - Mastite; Bovinos de leite; Dairy cattle; Diagnosis; Diagnostico; Diagnóstico microbiológico; Doenca animal; Gado de leite; Gado leiteiro; Infection; Laboratory diagnosis; Mamite; Mammary gland diseases; Mastite; Mastite - Diagnóstico; Mastitis; Microbiological diagnostic; Microbiological procedures; Microorganismo; Microorganisms; Pathogens; Patogeno; Rebanho. |
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Categoria do assunto: |
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Marc: |
LEADER 01951nam a2200505 a 4500
001 1009401
005 2015-12-17
008 1999 bl uuuu u0uu1 u #d
100 1 $aBRITO, M. A. V. P. e.
245 $aDiagnóstico microbiológico da mastite.
260 $aJuiz de Fora : Embrapa Gado de Leite$c1999
300 $a26 p.
490 $a(Embrapa Gado de Leite. Circular Tecnica, 55).
520 $aExames de casos clínicos; Cultura do leite para avaliação do rebanho; Cultura do leite total do rebanho; Coleta das amostras de leite; Identificação dos patógenos da mastite; Cocos gram-positivos, catalase positivos; Cocos gram-positivos, catalase negativos; Bactérias gram-negativas; Bastonetes gram-positivos; Outros micro organismos; Amostras contaminadas; Antibiograma; Procedimentos para a coleta de leite para exame microbiológicos; Material necessário; Quando coletar as amostras; Preparação das tetas; Coleta das amostras; Manutenção das amostras; Informações sobre as amostras.
653 $aAnalise de laboratorio
653 $aAnimal diseases
653 $aBacteria
653 $aBacterioses
653 $aBovine mastitis
653 $aBovino
653 $aBovino de leite
653 $aBovino de Leite - Mastite
653 $aBovinos de leite
653 $aDairy cattle
653 $aDiagnosis
653 $aDiagnostico
653 $aDiagnóstico microbiológico
653 $aDoenca animal
653 $aGado de leite
653 $aGado leiteiro
653 $aInfection
653 $aLaboratory diagnosis
653 $aMamite
653 $aMammary gland diseases
653 $aMastite
653 $aMastite - Diagnóstico
653 $aMastitis
653 $aMicrobiological diagnostic
653 $aMicrobiological procedures
653 $aMicroorganismo
653 $aMicroorganisms
653 $aPathogens
653 $aPatogeno
653 $aRebanho
700 1 $aBRITO, J. R. F.
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Registro original: |
Biblioteca Rui Tendinha (BRT) |
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Registro Completo |
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Biblioteca(s): |
Biblioteca Rui Tendinha. |
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Data corrente: |
29/07/2019 |
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Data da última atualização: |
29/07/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
ANTUNES, T. F. S.; AMARAL, R. J. V.; VENTURA, J. A.; GODINHO, M. T.; AMARAL, J. G.; SOUZA, F. O.; ZERBINI, P. A.; FERNANDES, P. M. B. |
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Afiliação: |
Tathiana Ferreira Sá Antunes, UFES; Raquel J. Vionette Amaral, UFES; Jose Aires Ventura, Incaper; Marcio Tadeu Godinho, UFV; Josiane G. Amaral, UFV; Flávia O. Souza, UFV; Poliane Alfenas Zerbini, UFV; Patricia Machado Bueno Fernandes, UFES. |
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Título: |
The dsRNA Virus Papaya Meleira Virus and an ssRNA Virus Are Associated with Papaya Sticky Disease. |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
PLoS ONE, v. 11, n. 5, e0155240. |
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Idioma: |
Português |
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Conteúdo: |
Papaya sticky disease, or ?meleira?, is one of the major diseases of papaya in Brazil and Mexico, capable of causing complete crop loss. The causal agent of sticky disease was identified as an isometric virus with a double stranded RNA (dsRNA) genome, named papaya meleira virus (PMeV). In the present study, PMeV dsRNA and a second RNA band of approximately 4.5 kb, both isolated from latex of papaya plants with severe symptoms of sticky disease, were deep-sequenced. The nearly complete sequence obtained for PMeV dsRNA is 8,814 nucleotides long and contains two putative ORFs; the predicted ORF1 and
ORF2 display similarity to capsid proteins and RdRp's, respectively, from mycoviruses tentatively classified in the family Totiviridae. The sequence obtained for the second RNA is 4,515 nucleotides long and contains two putative ORFs. The predicted ORFs 1 and 2 display 48% and 73% sequence identity, respectively, with the corresponding proteins of papaya virus Q, an umbravirus recently described infecting papaya in Ecuador. Viral purification in a sucrose gradient allowed separation of particles containing each RNA. Mass spectrometry analysis indicated that both PMeV and the second RNA virus (named papaya
meleira virus 2, PMeV2) were encapsidated in particles formed by the protein encoded by PMeV ORF1. The presence of both PMeV and PMeV2 was confirmed in field plants showing typical symptoms of sticky disease. Interestingly, PMeV was detected alone in asymptomatic plants. Together, our results indicate that sticky disease is associated with double infection by PMeV and PMeV2. MenosPapaya sticky disease, or ?meleira?, is one of the major diseases of papaya in Brazil and Mexico, capable of causing complete crop loss. The causal agent of sticky disease was identified as an isometric virus with a double stranded RNA (dsRNA) genome, named papaya meleira virus (PMeV). In the present study, PMeV dsRNA and a second RNA band of approximately 4.5 kb, both isolated from latex of papaya plants with severe symptoms of sticky disease, were deep-sequenced. The nearly complete sequence obtained for PMeV dsRNA is 8,814 nucleotides long and contains two putative ORFs; the predicted ORF1 and
ORF2 display similarity to capsid proteins and RdRp's, respectively, from mycoviruses tentatively classified in the family Totiviridae. The sequence obtained for the second RNA is 4,515 nucleotides long and contains two putative ORFs. The predicted ORFs 1 and 2 display 48% and 73% sequence identity, respectively, with the corresponding proteins of papaya virus Q, an umbravirus recently described infecting papaya in Ecuador. Viral purification in a sucrose gradient allowed separation of particles containing each RNA. Mass spectrometry analysis indicated that both PMeV and the second RNA virus (named papaya
meleira virus 2, PMeV2) were encapsidated in particles formed by the protein encoded by PMeV ORF1. The presence of both PMeV and PMeV2 was confirmed in field plants showing typical symptoms of sticky disease. Interestingly, PMeV was detected alone in asymptomatic plants. Together, ... Mostrar Tudo |
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Palavras-Chave: |
Mamão; Mamoeiro; Meleira; Papaya Meleira Virus. |
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Categoria do assunto: |
G Melhoramento Genético |
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URL: |
https://biblioteca.incaper.es.gov.br/digital/bitstream/123456789/3645/1/the-dsrna-papaya-ventura.PDF
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Marc: |
LEADER 02305naa a2200253 a 4500
001 1021491
005 2019-07-29
008 2016 bl uuuu u00u1 u #d
100 1 $aANTUNES, T. F. S.
245 $aThe dsRNA Virus Papaya Meleira Virus and an ssRNA Virus Are Associated with Papaya Sticky Disease.$h[electronic resource]
260 $c2016
520 $aPapaya sticky disease, or ?meleira?, is one of the major diseases of papaya in Brazil and Mexico, capable of causing complete crop loss. The causal agent of sticky disease was identified as an isometric virus with a double stranded RNA (dsRNA) genome, named papaya meleira virus (PMeV). In the present study, PMeV dsRNA and a second RNA band of approximately 4.5 kb, both isolated from latex of papaya plants with severe symptoms of sticky disease, were deep-sequenced. The nearly complete sequence obtained for PMeV dsRNA is 8,814 nucleotides long and contains two putative ORFs; the predicted ORF1 and ORF2 display similarity to capsid proteins and RdRp's, respectively, from mycoviruses tentatively classified in the family Totiviridae. The sequence obtained for the second RNA is 4,515 nucleotides long and contains two putative ORFs. The predicted ORFs 1 and 2 display 48% and 73% sequence identity, respectively, with the corresponding proteins of papaya virus Q, an umbravirus recently described infecting papaya in Ecuador. Viral purification in a sucrose gradient allowed separation of particles containing each RNA. Mass spectrometry analysis indicated that both PMeV and the second RNA virus (named papaya meleira virus 2, PMeV2) were encapsidated in particles formed by the protein encoded by PMeV ORF1. The presence of both PMeV and PMeV2 was confirmed in field plants showing typical symptoms of sticky disease. Interestingly, PMeV was detected alone in asymptomatic plants. Together, our results indicate that sticky disease is associated with double infection by PMeV and PMeV2.
653 $aMamão
653 $aMamoeiro
653 $aMeleira
653 $aPapaya Meleira Virus
700 1 $aAMARAL, R. J. V.
700 1 $aVENTURA, J. A.
700 1 $aGODINHO, M. T.
700 1 $aAMARAL, J. G.
700 1 $aSOUZA, F. O.
700 1 $aZERBINI, P. A.
700 1 $aFERNANDES, P. M. B.
773 $tPLoS ONE$gv. 11, n. 5, e0155240.
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