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Registro Completo |
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Biblioteca(s): |
Biblioteca Rui Tendinha. |
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Data corrente: |
09/09/2020 |
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Data da última atualização: |
09/09/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
DUARTE, M. F.; PEREIRA-CARVALHO, R. de C.; REIS, L. de N. A. dos; ROJAS, M. J.; GILBERTSON, R.; COSTA, H.; BOITEUX, L. S.; FONSECA, M. E. N. |
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Afiliação: |
Macaria Ferreira Duarte, UnB; Rita de Cássia Pereira Carvalho, UnB; Luciane de Nazaré Almeida dos Reis, UnB; Maria J. Rojas, UC-DAVIS; Robert Gilbertson, UC-DAVIS; Helcio Costa, Incaper; Leonardo Silva Boiteux, EMBRAPA; Maria Esther N. Fonseca, EMBRAPA. |
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Título: |
Natural Infection of Tomatoes (Solanum lycopersicum) by Euphorbia yellow mosaic virus Isolates across Four Brazilian States. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Plant Disease, set. 2020. |
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Idioma: |
Inglês |
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Conteúdo: |
Severe yield losses induced by a complex of whitefly?transmitted Begomovirus species (family Geminiviridae) have been reported in tomatoes in Brazil (Reis et al. 2020). Nine isolates were obtained from tomato plants exhibiting begomovirus?like symptoms (viz. apical and interveinal chlorosis, yellow spots, and stunting) during independent field surveys: one isolate in Sumaré, São Paulo?SP State (isolate SP?066) in 2001, two in Serra Negra, Minas Gerais?MG (MG?012 and MG?016) in 2002, five in Caxias do Sul, Rio Grande do Sul?RS (RS?039, RS?045, RS?046, RS?047 and RS?058) in 2011 and one in Domingos Martins, Espírito Santo?ES (ES?148) in 2016. Disease incidence across all sampled fields ranged from 30% (in Domingos Martins?ES) to 90% in Sumaré?SP. Total DNA extraction was done by a modified CTAB method (Boiteux et al., 1999). Begomovirus infection was confirmed in all isolates by selective amplification of viral DNA?A segments using the primer pairs ?PAL1v1978 / PAR1c496? (Rojas et al., 1993) and ?BegomoAFor1? / ?BegomoARev1? (Ha et al., 2006), which produce two large and non?overlapping segments (≈1120 bp and ≈1205 bp, respectively). These PCR amplicons were initially characterized via direct Sanger dideoxy sequencing at CNPH. BLASTn analysis of the partial DNA?A genomes of these nine isolates indicated identity levels of 95?97% to three euphorbia yellow mosaic virus (EuYMV) reference isolates (= KY559532, JF756674, and KY559583) found infecting the weed Euphorbia heterophylla L. The entire DNA?A (2,609 nts = MN746971) and DNA?B (2,579 nts = MN746970) components of the MG?016 isolate were obtained via high?performance sequencing using Illumina HiSeq 2500 system (Macrogen Inc., South Korea). Sequences were assembled with the CLC Genomics Workbench program 10. Contigs were validated by BLASTx and BLASTn and compared to the ssDNA virus database at NCBI (www.ncbi.nlm.nih.gov). The fully?characterized MG?016 isolate displayed identity levels ranging from 97 to 99% to the EuYMV reference isolates as well as similar genomic features such as the conserved TATA box, nonanucleotide, and iterons (that were in agreement with a cognate nature of the DNA?A and DNA?B components). A partial sequence of the DNA?B genome was also obtained for the MG?012 isolate (MT7831942). The isolates MG?012 and MG?016 were found in mixed infections with tomato severe rugose virus (ToSRV) and tomato golden vein virus (TGVV), respectively. In addition, the complete DNA?A genomes of ES?148 (MN746972) and SP?066 (MN782438) were also obtained via a combination of primer walking and Sanger dideoxy sequencing, displaying 96?98% identity to EuYMV isolates. To our knowledge, this is the first report of multiple and independent events of natural infection of tomatoes by EuYMV isolates. Our results confirm the natural host status of tomatoes to EuYMV isolates as indicated in previous infectivity assays using biolistic inoculation (Barreto et al., 2013). The weed E. heterophylla is widely disseminated and very often present within tomato fields due to its higher levels of tolerance to the major herbicide (metribuzin) employed in this crop. Therefore, this weed may act as a persistent reservoir of tomato?infecting EuYMV isolates, which may allow the selection of viral populations potentially more adapted to this vegetable crop. MenosSevere yield losses induced by a complex of whitefly?transmitted Begomovirus species (family Geminiviridae) have been reported in tomatoes in Brazil (Reis et al. 2020). Nine isolates were obtained from tomato plants exhibiting begomovirus?like symptoms (viz. apical and interveinal chlorosis, yellow spots, and stunting) during independent field surveys: one isolate in Sumaré, São Paulo?SP State (isolate SP?066) in 2001, two in Serra Negra, Minas Gerais?MG (MG?012 and MG?016) in 2002, five in Caxias do Sul, Rio Grande do Sul?RS (RS?039, RS?045, RS?046, RS?047 and RS?058) in 2011 and one in Domingos Martins, Espírito Santo?ES (ES?148) in 2016. Disease incidence across all sampled fields ranged from 30% (in Domingos Martins?ES) to 90% in Sumaré?SP. Total DNA extraction was done by a modified CTAB method (Boiteux et al., 1999). Begomovirus infection was confirmed in all isolates by selective amplification of viral DNA?A segments using the primer pairs ?PAL1v1978 / PAR1c496? (Rojas et al., 1993) and ?BegomoAFor1? / ?BegomoARev1? (Ha et al., 2006), which produce two large and non?overlapping segments (≈1120 bp and ≈1205 bp, respectively). These PCR amplicons were initially characterized via direct Sanger dideoxy sequencing at CNPH. BLASTn analysis of the partial DNA?A genomes of these nine isolates indicated identity levels of 95?97% to three euphorbia yellow mosaic virus (EuYMV) reference isolates (= KY559532, JF756674, and KY559583) found infecting the weed Euphorbia ... Mostrar Tudo |
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Thesagro: |
Doença de Planta; Erva Daninha; Mosaico; Tomate. |
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Thesaurus NAL: |
Begomovirus. |
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Categoria do assunto: |
H Saúde e Patologia |
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URL: |
https://biblioteca.incaper.es.gov.br/digital/bitstream/123456789/4073/1/natural-infection-tomatoes-costa.pdf
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Marc: |
LEADER 04125naa a2200265 a 4500
001 1022277
005 2020-09-09
008 2020 bl uuuu u00u1 u #d
100 1 $aDUARTE, M. F.
245 $aNatural Infection of Tomatoes (Solanum lycopersicum) by Euphorbia yellow mosaic virus Isolates across Four Brazilian States.$h[electronic resource]
260 $c2020
520 $aSevere yield losses induced by a complex of whitefly?transmitted Begomovirus species (family Geminiviridae) have been reported in tomatoes in Brazil (Reis et al. 2020). Nine isolates were obtained from tomato plants exhibiting begomovirus?like symptoms (viz. apical and interveinal chlorosis, yellow spots, and stunting) during independent field surveys: one isolate in Sumaré, São Paulo?SP State (isolate SP?066) in 2001, two in Serra Negra, Minas Gerais?MG (MG?012 and MG?016) in 2002, five in Caxias do Sul, Rio Grande do Sul?RS (RS?039, RS?045, RS?046, RS?047 and RS?058) in 2011 and one in Domingos Martins, Espírito Santo?ES (ES?148) in 2016. Disease incidence across all sampled fields ranged from 30% (in Domingos Martins?ES) to 90% in Sumaré?SP. Total DNA extraction was done by a modified CTAB method (Boiteux et al., 1999). Begomovirus infection was confirmed in all isolates by selective amplification of viral DNA?A segments using the primer pairs ?PAL1v1978 / PAR1c496? (Rojas et al., 1993) and ?BegomoAFor1? / ?BegomoARev1? (Ha et al., 2006), which produce two large and non?overlapping segments (≈1120 bp and ≈1205 bp, respectively). These PCR amplicons were initially characterized via direct Sanger dideoxy sequencing at CNPH. BLASTn analysis of the partial DNA?A genomes of these nine isolates indicated identity levels of 95?97% to three euphorbia yellow mosaic virus (EuYMV) reference isolates (= KY559532, JF756674, and KY559583) found infecting the weed Euphorbia heterophylla L. The entire DNA?A (2,609 nts = MN746971) and DNA?B (2,579 nts = MN746970) components of the MG?016 isolate were obtained via high?performance sequencing using Illumina HiSeq 2500 system (Macrogen Inc., South Korea). Sequences were assembled with the CLC Genomics Workbench program 10. Contigs were validated by BLASTx and BLASTn and compared to the ssDNA virus database at NCBI (www.ncbi.nlm.nih.gov). The fully?characterized MG?016 isolate displayed identity levels ranging from 97 to 99% to the EuYMV reference isolates as well as similar genomic features such as the conserved TATA box, nonanucleotide, and iterons (that were in agreement with a cognate nature of the DNA?A and DNA?B components). A partial sequence of the DNA?B genome was also obtained for the MG?012 isolate (MT7831942). The isolates MG?012 and MG?016 were found in mixed infections with tomato severe rugose virus (ToSRV) and tomato golden vein virus (TGVV), respectively. In addition, the complete DNA?A genomes of ES?148 (MN746972) and SP?066 (MN782438) were also obtained via a combination of primer walking and Sanger dideoxy sequencing, displaying 96?98% identity to EuYMV isolates. To our knowledge, this is the first report of multiple and independent events of natural infection of tomatoes by EuYMV isolates. Our results confirm the natural host status of tomatoes to EuYMV isolates as indicated in previous infectivity assays using biolistic inoculation (Barreto et al., 2013). The weed E. heterophylla is widely disseminated and very often present within tomato fields due to its higher levels of tolerance to the major herbicide (metribuzin) employed in this crop. Therefore, this weed may act as a persistent reservoir of tomato?infecting EuYMV isolates, which may allow the selection of viral populations potentially more adapted to this vegetable crop.
650 $aBegomovirus
650 $aDoença de Planta
650 $aErva Daninha
650 $aMosaico
650 $aTomate
700 1 $aPEREIRA-CARVALHO, R. de C.
700 1 $aREIS, L. de N. A. dos
700 1 $aROJAS, M. J.
700 1 $aGILBERTSON, R.
700 1 $aCOSTA, H.
700 1 $aBOITEUX, L. S.
700 1 $aFONSECA, M. E. N.
773 $tPlant Disease, set. 2020.
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Registro original: |
Biblioteca Rui Tendinha (BRT) |
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Fechar
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Registro Completo |
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Biblioteca(s): |
Biblioteca Rui Tendinha. |
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Data corrente: |
14/10/2019 |
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Data da última atualização: |
14/10/2019 |
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Tipo da produção científica: |
Publicação em Anais de Congresso |
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Autoria: |
SOUZA, G. A. R. de; PIRES, I. D.; CERRI NETO, B.; COSTA, R. J.; CORREIA, L. Z.; FERREIRA, T. R.; LIMA, K. C. C.; SILVA, F. R. N. e; MACHADO FILHO, J. A.; DOUSSEAU, S.; FALQUETO, A. R.; ARANTES, L. de O. |
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Afiliação: |
Guilherme Augusto Rodrigues de Souza, UENF; Igor Damasceno Pires, UFES/CEUNES; Basílio Cerri Neto, UFES; Rizia Joyce Costa, Incaper; Laísa Zanelato Correia, UENF; Thayanne Rangel Ferreira, UFES/CEUNES; Kayo Cesar Corrêa Lima, Incaper; Fernanda Rodrigues Nunes e Silva, UFES; José Altino Machado Filho, Incaper; Sara Dousseau Arantes, Incaper; Antelmo Ralph Falqueto, UFES; Lucio de Oliveira Arantes, Incaper. |
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Título: |
Caracterização fotoquímica de clones de Coffea canephora Pierre ex Froehner cultivados em condições de pleno sol. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
In: SIMPÓSIO DE PESQUISA DOS CAFÉS DO BRASIL, 10., 2019, Vitória. Pesquisa, inovação e sustentabilidade dos cafés do Brasil: anais... Vitória: Consórcio Pesquisa Café, 2019. |
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Idioma: |
Português |
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Conteúdo: |
O objetivo deste trabalho foi avaliar seis clones de C. canephora Pierre ex Froehner cultivados sob condições de pleno sol, a fim de caracterizá-los de acordo com suas respostas fotoquímicas, obtidas por meio da análise da fluorescência da clorofila a. Para isso, o experimento foi conduzido na Fazenda Experimental do Incaper, localizada no munícipio de Sooretama ? ES, onde foram dispostos quatro blocos, dentro dos quais foram cultivados aleatoriamente os seis clones supracitados, caracterizando um delineamento em blocos casualizados. Para avaliação da emissão de fluorescência, foram selecionadas folhas pertencentes ao terceiro ou quarto par de folhas completamente expandidas em ramos plagiotrópicos do terço superior das plantas de café. Em cada parcela experimental foram selecionadas três plantas aleatórias, nas quais as medições foram efetuadas. Antes das medições, as folhas selecionadas foram adaptadas ao escuro por meio da utilização de pinças foliares específicas para este procedimento, e após 30 minutos de adaptação foram submetidas a um pulso de luz saturante de 3500 μmol de fótons m-2 s-1, utilizando-se o Handy-PEA (Hansatech instruments). Os dados coletados foram processados e submetidos às normalizações propostas por Strasser e Strasser (1995) para avaliação da diferença cinética na curva OJIP para os seis clones. Os resultados mostraram que entre os clones avaliados, o clone 83 mostrou-se mais estável para fluxo de elétrons no PSII, isso porque cultivado em pleno sol ele manteve amplitudes negativas para as normalizações ΔVOP, ΔVOJ, ΔVOK e ΔVOI, enquanto os demais clones manifestaram amplitudes positivas, sendo o clone 16 o mais significativo. Já para a taxa de redução global de aceptores do PSI (ΔVIP) o clone 83 não se mostrou tão eficiente. Mas em termos gerais o clone 83 demonstrou, a partir de suas características fotoquímicas, maior potencial para cultivo a pleno sol.
The objective of this work was to evaluate six C. canephora Pierre ex Froehner clones cultivated under full sun conditions in order to characterize them according to their photochemical responses, obtained by chlorophyll a fluorescence analysis. For this, the experiment was conducted at Incaper Experimental Farm, located in the municipality of Sooretama - ES, where four blocks were arranged, within which the six aforementioned clones were randomly cultivated, characterizing a randomized block design. For fluorescence emission evaluation, leaves belonging to the third or fourth pair of fully expanded leaves in plagiotropic branches of the upper third of coffee plants were selected. In each experimental plot three random plants were selected, in which measurements were made. Before the measurements, the selected leaves were adapted to the dark by the use of specific leaf clamps for this procedure, and after 30 minutes of adaptation they were submitted to a 3500 μmol photon m-2 s-1 saturating light pulse using Handy-PEA (Hansatech instruments). The collected data were processed and submitted to the normalization proposed by Strasser and Strasser (1995) to evaluate the kinetic difference in the OJIP curve for the six clones. The results showed that among the evaluated clones, clone 83 was more stable for electron flux in the PSII, because in full sun it maintained negative amplitudes for the normalization ΔVOP, ΔVOJ, ΔVOK and ΔVOI, while the other clones showed positive amplitudes, being clone 16 the most significant. For the overall PSI acceptor reduction rate (ΔVIP), clone 83 was not as efficient. But in general terms clone 83 demonstrated, from its photochemical characteristics, greater potential for full sun cultivation. MenosO objetivo deste trabalho foi avaliar seis clones de C. canephora Pierre ex Froehner cultivados sob condições de pleno sol, a fim de caracterizá-los de acordo com suas respostas fotoquímicas, obtidas por meio da análise da fluorescência da clorofila a. Para isso, o experimento foi conduzido na Fazenda Experimental do Incaper, localizada no munícipio de Sooretama ? ES, onde foram dispostos quatro blocos, dentro dos quais foram cultivados aleatoriamente os seis clones supracitados, caracterizando um delineamento em blocos casualizados. Para avaliação da emissão de fluorescência, foram selecionadas folhas pertencentes ao terceiro ou quarto par de folhas completamente expandidas em ramos plagiotrópicos do terço superior das plantas de café. Em cada parcela experimental foram selecionadas três plantas aleatórias, nas quais as medições foram efetuadas. Antes das medições, as folhas selecionadas foram adaptadas ao escuro por meio da utilização de pinças foliares específicas para este procedimento, e após 30 minutos de adaptação foram submetidas a um pulso de luz saturante de 3500 μmol de fótons m-2 s-1, utilizando-se o Handy-PEA (Hansatech instruments). Os dados coletados foram processados e submetidos às normalizações propostas por Strasser e Strasser (1995) para avaliação da diferença cinética na curva OJIP para os seis clones. Os resultados mostraram que entre os clones avaliados, o clone 83 mostrou-se mais estável para fluxo de elétrons no PSII, isso porque cultivado em pl... Mostrar Tudo |
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Palavras-Chave: |
Café Conilon; Desempenho fotoquímico; Estresse luminoso; Fluorescência da clorofila. |
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Thesaurus NAL: |
Chlorophyll a fluorescence; Conilon coffee; Light stress; Photochemical performance. |
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Categoria do assunto: |
-- |
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URL: |
https://biblioteca.incaper.es.gov.br/digital/bitstream/123456789/3872/1/fotoquimica-clones-costa.pdf
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Marc: |
LEADER 04945nam a2200337 a 4500
001 1021847
005 2019-10-14
008 2019 bl uuuu u01u1 u #d
100 1 $aSOUZA, G. A. R. de
245 $aCaracterização fotoquímica de clones de Coffea canephora Pierre ex Froehner cultivados em condições de pleno sol.$h[electronic resource]
260 $aIn: SIMPÓSIO DE PESQUISA DOS CAFÉS DO BRASIL, 10., 2019, Vitória. Pesquisa, inovação e sustentabilidade dos cafés do Brasil: anais... Vitória: Consórcio Pesquisa Café$c2019
520 $aO objetivo deste trabalho foi avaliar seis clones de C. canephora Pierre ex Froehner cultivados sob condições de pleno sol, a fim de caracterizá-los de acordo com suas respostas fotoquímicas, obtidas por meio da análise da fluorescência da clorofila a. Para isso, o experimento foi conduzido na Fazenda Experimental do Incaper, localizada no munícipio de Sooretama ? ES, onde foram dispostos quatro blocos, dentro dos quais foram cultivados aleatoriamente os seis clones supracitados, caracterizando um delineamento em blocos casualizados. Para avaliação da emissão de fluorescência, foram selecionadas folhas pertencentes ao terceiro ou quarto par de folhas completamente expandidas em ramos plagiotrópicos do terço superior das plantas de café. Em cada parcela experimental foram selecionadas três plantas aleatórias, nas quais as medições foram efetuadas. Antes das medições, as folhas selecionadas foram adaptadas ao escuro por meio da utilização de pinças foliares específicas para este procedimento, e após 30 minutos de adaptação foram submetidas a um pulso de luz saturante de 3500 μmol de fótons m-2 s-1, utilizando-se o Handy-PEA (Hansatech instruments). Os dados coletados foram processados e submetidos às normalizações propostas por Strasser e Strasser (1995) para avaliação da diferença cinética na curva OJIP para os seis clones. Os resultados mostraram que entre os clones avaliados, o clone 83 mostrou-se mais estável para fluxo de elétrons no PSII, isso porque cultivado em pleno sol ele manteve amplitudes negativas para as normalizações ΔVOP, ΔVOJ, ΔVOK e ΔVOI, enquanto os demais clones manifestaram amplitudes positivas, sendo o clone 16 o mais significativo. Já para a taxa de redução global de aceptores do PSI (ΔVIP) o clone 83 não se mostrou tão eficiente. Mas em termos gerais o clone 83 demonstrou, a partir de suas características fotoquímicas, maior potencial para cultivo a pleno sol. The objective of this work was to evaluate six C. canephora Pierre ex Froehner clones cultivated under full sun conditions in order to characterize them according to their photochemical responses, obtained by chlorophyll a fluorescence analysis. For this, the experiment was conducted at Incaper Experimental Farm, located in the municipality of Sooretama - ES, where four blocks were arranged, within which the six aforementioned clones were randomly cultivated, characterizing a randomized block design. For fluorescence emission evaluation, leaves belonging to the third or fourth pair of fully expanded leaves in plagiotropic branches of the upper third of coffee plants were selected. In each experimental plot three random plants were selected, in which measurements were made. Before the measurements, the selected leaves were adapted to the dark by the use of specific leaf clamps for this procedure, and after 30 minutes of adaptation they were submitted to a 3500 μmol photon m-2 s-1 saturating light pulse using Handy-PEA (Hansatech instruments). The collected data were processed and submitted to the normalization proposed by Strasser and Strasser (1995) to evaluate the kinetic difference in the OJIP curve for the six clones. The results showed that among the evaluated clones, clone 83 was more stable for electron flux in the PSII, because in full sun it maintained negative amplitudes for the normalization ΔVOP, ΔVOJ, ΔVOK and ΔVOI, while the other clones showed positive amplitudes, being clone 16 the most significant. For the overall PSI acceptor reduction rate (ΔVIP), clone 83 was not as efficient. But in general terms clone 83 demonstrated, from its photochemical characteristics, greater potential for full sun cultivation.
650 $aChlorophyll a fluorescence
650 $aConilon coffee
650 $aLight stress
650 $aPhotochemical performance
653 $aCafé Conilon
653 $aDesempenho fotoquímico
653 $aEstresse luminoso
653 $aFluorescência da clorofila
700 1 $aPIRES, I. D.
700 1 $aCERRI NETO, B.
700 1 $aCOSTA, R. J.
700 1 $aCORREIA, L. Z.
700 1 $aFERREIRA, T. R.
700 1 $aLIMA, K. C. C.
700 1 $aSILVA, F. R. N. e
700 1 $aMACHADO FILHO, J. A.
700 1 $aDOUSSEAU, S.
700 1 $aFALQUETO, A. R.
700 1 $aARANTES, L. de O.
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