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Registro Completo |
Biblioteca(s): |
Biblioteca Rui Tendinha. |
Data corrente: |
10/02/2014 |
Data da última atualização: |
10/02/2014 |
Autoria: |
Emater-ES. |
Título: |
A visão de um pequeno grande produtor rural. |
Ano de publicação: |
19 |
Fonte/Imprenta: |
[Vila Valério, ES] : EMATER-ES, 19--. |
Descrição Física: |
DVD (25' 55'') : son., color. |
Idioma: |
Português |
Conteúdo: |
Ver e ouvir alguns depoimentos de sucesso encontrados pelo produtor rural Ozilio Parteli em sua pequena propriedade em Vila Valério. |
Palavras-Chave: |
Café conilon; Caminho do sucesso; EMATER-ES; Emcapa; Empresa; Espírito Santo (Estado); Norte do Espírito Santo; Produção; Produtor rural; Qualidade; Qualidade de vida; Sistema de trabalho; Vila Valério; Visão. |
Categoria do assunto: |
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Marc: |
LEADER 00893ngm a2200289 a 4500 001 1001911 005 2014-02-10 008 bl --- 0-- m #d 100 1 $aEmater-ES. 245 $aA visão de um pequeno grande produtor rural. 260 $a[Vila Valério, ES] : EMATER-ES, 19--.$c19 300 $cDVD (25' 55'') : son., color. 520 $aVer e ouvir alguns depoimentos de sucesso encontrados pelo produtor rural Ozilio Parteli em sua pequena propriedade em Vila Valério. 653 $aCafé conilon 653 $aCaminho do sucesso 653 $aEMATER-ES 653 $aEmcapa 653 $aEmpresa 653 $aEspírito Santo (Estado) 653 $aNorte do Espírito Santo 653 $aProdução 653 $aProdutor rural 653 $aQualidade 653 $aQualidade de vida 653 $aSistema de trabalho 653 $aVila Valério 653 $aVisão
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Registro original: |
Biblioteca Rui Tendinha (BRT) |
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Registro Completo |
Biblioteca(s): |
Biblioteca Rui Tendinha. |
Data corrente: |
25/09/2014 |
Data da última atualização: |
01/09/2015 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 1 |
Autoria: |
ABREU, P. M. V.; GASPAR, C. G.; BUSS, D. S.; VENTURA, J. A.; FERREIRA, P. C. G.; FERNANDES, P. M. B. |
Afiliação: |
Paolla M. V. Abreu, Universidade Federal do Espíríto Santo; Clicia G. Gaspar, Univervidade Federal do Rio de Janeiro; David S. Buss, Universidade Federal do Espírito Santo; Jose Aires Ventura, Incaper; Paulo C. G. Ferreira, Universidade Federal do Rio de Janeiro; Patricia M. B. Fernandes, Universidade Federal do Espírito Santo. |
Título: |
Carica papaya MicroRNAs Are Responsive to Papaya meleira virus Infection. |
Ano de publicação: |
2014 |
Fonte/Imprenta: |
PLOS ONE, v. 9, issue 7, p. 1-13, july 2014. |
Páginas: |
13 p. |
Descrição Física: |
il., color. |
ISSN: |
1932-6203 |
DOI: |
10.1371 |
Idioma: |
Português |
Conteúdo: |
MicroRNAs are implicated in the response to biotic stresses. Papaya meleira virus (PMeV) is the causal agent of sticky disease, a commercially important pathology in papaya for which there are currently no resistant varieties. PMeV has a number of unusual features, such as residence in the laticifers of infected plants, and the response of the papaya to PMeV infection is not well understood. The protein levels of 20S proteasome subunits increase during PMeV infection, suggesting that proteolysis could be an important aspect of the plant defense response mechanism. To date, 10,598 plant microRNAs have been identified in the Plant miRNAs Database, but only two, miR162 and miR403, are from papaya. In this study, known plant microRNA sequences were used to search for potential microRNAs in the papaya genome. A total of 462 microRNAs, representing 72 microRNA families, were identified. The expression of 11 microRNAs, whose targets are involved in 20S and 26S proteasomal degradation and in other stress response pathways, was compared by real-time PCR in healthy and infected papaya leaf tissue. We found that the expression of miRNAs involved in proteasomal degradation increased in response to very low levels of PMeV titre and decreased as the viral titre increased. In contrast, miRNAs implicated in the plant response to biotic stress decreased their expression at very low level of PMeV and increased at high PMeV levels. Corroborating with this results, analysed target genes for this miRNAs had their expression modulated in a dependent manner. This study represents a comprehensive identification of conserved miRNAs inpapaya. The data presented here might help to complement the available molecular and genomic tools for the study of papaya. The differential expression of some miRNAs and identifying their target genes will be helpful for understanding the regulation and interaction of PMeV and papaya. MenosMicroRNAs are implicated in the response to biotic stresses. Papaya meleira virus (PMeV) is the causal agent of sticky disease, a commercially important pathology in papaya for which there are currently no resistant varieties. PMeV has a number of unusual features, such as residence in the laticifers of infected plants, and the response of the papaya to PMeV infection is not well understood. The protein levels of 20S proteasome subunits increase during PMeV infection, suggesting that proteolysis could be an important aspect of the plant defense response mechanism. To date, 10,598 plant microRNAs have been identified in the Plant miRNAs Database, but only two, miR162 and miR403, are from papaya. In this study, known plant microRNA sequences were used to search for potential microRNAs in the papaya genome. A total of 462 microRNAs, representing 72 microRNA families, were identified. The expression of 11 microRNAs, whose targets are involved in 20S and 26S proteasomal degradation and in other stress response pathways, was compared by real-time PCR in healthy and infected papaya leaf tissue. We found that the expression of miRNAs involved in proteasomal degradation increased in response to very low levels of PMeV titre and decreased as the viral titre increased. In contrast, miRNAs implicated in the plant response to biotic stress decreased their expression at very low level of PMeV and increased at high PMeV levels. Corroborating with this results, analysed target genes for thi... Mostrar Tudo |
Palavras-Chave: |
Carica papaya; Doenças; Mamão; Meleira; MiRNAs; PMeV; Vírus. |
Thesaurus NAL: |
Carica papaya; Disease; Meleira; MiRNAs; PMeV; Virus. |
Categoria do assunto: |
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URL: |
http://biblioteca.incaper.es.gov.br/digital/bitstream/item/397/1/Carica-papaya-microRNAS-Are-responsive-to-papaya.pdf
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Marc: |
LEADER 02852naa a2200373 a 4500 001 1004325 005 2015-09-01 008 2014 bl uuuu u00u1 u #d 022 $a1932-6203 024 7 $a10.1371$2DOI 100 1 $aABREU, P. M. V. 245 $aCarica papaya MicroRNAs Are Responsive to Papaya meleira virus Infection.$h[electronic resource] 260 $c2014 300 $a13 p.$cil., color. 520 $aMicroRNAs are implicated in the response to biotic stresses. Papaya meleira virus (PMeV) is the causal agent of sticky disease, a commercially important pathology in papaya for which there are currently no resistant varieties. PMeV has a number of unusual features, such as residence in the laticifers of infected plants, and the response of the papaya to PMeV infection is not well understood. The protein levels of 20S proteasome subunits increase during PMeV infection, suggesting that proteolysis could be an important aspect of the plant defense response mechanism. To date, 10,598 plant microRNAs have been identified in the Plant miRNAs Database, but only two, miR162 and miR403, are from papaya. In this study, known plant microRNA sequences were used to search for potential microRNAs in the papaya genome. A total of 462 microRNAs, representing 72 microRNA families, were identified. The expression of 11 microRNAs, whose targets are involved in 20S and 26S proteasomal degradation and in other stress response pathways, was compared by real-time PCR in healthy and infected papaya leaf tissue. We found that the expression of miRNAs involved in proteasomal degradation increased in response to very low levels of PMeV titre and decreased as the viral titre increased. In contrast, miRNAs implicated in the plant response to biotic stress decreased their expression at very low level of PMeV and increased at high PMeV levels. Corroborating with this results, analysed target genes for this miRNAs had their expression modulated in a dependent manner. This study represents a comprehensive identification of conserved miRNAs inpapaya. The data presented here might help to complement the available molecular and genomic tools for the study of papaya. The differential expression of some miRNAs and identifying their target genes will be helpful for understanding the regulation and interaction of PMeV and papaya. 650 $aCarica papaya 650 $aDisease 650 $aMeleira 650 $aMiRNAs 650 $aPMeV 650 $aVirus 653 $aCarica papaya 653 $aDoenças 653 $aMamão 653 $aMeleira 653 $aMiRNAs 653 $aPMeV 653 $aVírus 700 1 $aGASPAR, C. G. 700 1 $aBUSS, D. S. 700 1 $aVENTURA, J. A. 700 1 $aFERREIRA, P. C. G. 700 1 $aFERNANDES, P. M. B. 773 $tPLOS ONE$gv. 9, issue 7, p. 1-13, july 2014.
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