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Registro Completo |
Biblioteca(s): |
Biblioteca Rui Tendinha. |
Data corrente: |
29/07/2019 |
Data da última atualização: |
29/07/2019 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
ANTUNES, T. F. S.; AMARAL, R. J. V.; VENTURA, J. A.; GODINHO, M. T.; AMARAL, J. G.; SOUZA, F. O.; ZERBINI, P. A.; FERNANDES, P. M. B. |
Afiliação: |
Tathiana Ferreira Sá Antunes, UFES; Raquel J. Vionette Amaral, UFES; Jose Aires Ventura, Incaper; Marcio Tadeu Godinho, UFV; Josiane G. Amaral, UFV; Flávia O. Souza, UFV; Poliane Alfenas Zerbini, UFV; Patricia Machado Bueno Fernandes, UFES. |
Título: |
The dsRNA Virus Papaya Meleira Virus and an ssRNA Virus Are Associated with Papaya Sticky Disease. |
Ano de publicação: |
2016 |
Fonte/Imprenta: |
PLoS ONE, v. 11, n. 5, e0155240. |
Idioma: |
Português |
Conteúdo: |
Papaya sticky disease, or ?meleira?, is one of the major diseases of papaya in Brazil and Mexico, capable of causing complete crop loss. The causal agent of sticky disease was identified as an isometric virus with a double stranded RNA (dsRNA) genome, named papaya meleira virus (PMeV). In the present study, PMeV dsRNA and a second RNA band of approximately 4.5 kb, both isolated from latex of papaya plants with severe symptoms of sticky disease, were deep-sequenced. The nearly complete sequence obtained for PMeV dsRNA is 8,814 nucleotides long and contains two putative ORFs; the predicted ORF1 and
ORF2 display similarity to capsid proteins and RdRp's, respectively, from mycoviruses tentatively classified in the family Totiviridae. The sequence obtained for the second RNA is 4,515 nucleotides long and contains two putative ORFs. The predicted ORFs 1 and 2 display 48% and 73% sequence identity, respectively, with the corresponding proteins of papaya virus Q, an umbravirus recently described infecting papaya in Ecuador. Viral purification in a sucrose gradient allowed separation of particles containing each RNA. Mass spectrometry analysis indicated that both PMeV and the second RNA virus (named papaya
meleira virus 2, PMeV2) were encapsidated in particles formed by the protein encoded by PMeV ORF1. The presence of both PMeV and PMeV2 was confirmed in field plants showing typical symptoms of sticky disease. Interestingly, PMeV was detected alone in asymptomatic plants. Together, our results indicate that sticky disease is associated with double infection by PMeV and PMeV2. MenosPapaya sticky disease, or ?meleira?, is one of the major diseases of papaya in Brazil and Mexico, capable of causing complete crop loss. The causal agent of sticky disease was identified as an isometric virus with a double stranded RNA (dsRNA) genome, named papaya meleira virus (PMeV). In the present study, PMeV dsRNA and a second RNA band of approximately 4.5 kb, both isolated from latex of papaya plants with severe symptoms of sticky disease, were deep-sequenced. The nearly complete sequence obtained for PMeV dsRNA is 8,814 nucleotides long and contains two putative ORFs; the predicted ORF1 and
ORF2 display similarity to capsid proteins and RdRp's, respectively, from mycoviruses tentatively classified in the family Totiviridae. The sequence obtained for the second RNA is 4,515 nucleotides long and contains two putative ORFs. The predicted ORFs 1 and 2 display 48% and 73% sequence identity, respectively, with the corresponding proteins of papaya virus Q, an umbravirus recently described infecting papaya in Ecuador. Viral purification in a sucrose gradient allowed separation of particles containing each RNA. Mass spectrometry analysis indicated that both PMeV and the second RNA virus (named papaya
meleira virus 2, PMeV2) were encapsidated in particles formed by the protein encoded by PMeV ORF1. The presence of both PMeV and PMeV2 was confirmed in field plants showing typical symptoms of sticky disease. Interestingly, PMeV was detected alone in asymptomatic plants. Together, ... Mostrar Tudo |
Palavras-Chave: |
Mamão; Mamoeiro; Meleira; Papaya Meleira Virus. |
Categoria do assunto: |
G Melhoramento Genético |
URL: |
https://biblioteca.incaper.es.gov.br/digital/bitstream/123456789/3645/1/the-dsrna-papaya-ventura.PDF
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Marc: |
LEADER 02305naa a2200253 a 4500 001 1021491 005 2019-07-29 008 2016 bl uuuu u00u1 u #d 100 1 $aANTUNES, T. F. S. 245 $aThe dsRNA Virus Papaya Meleira Virus and an ssRNA Virus Are Associated with Papaya Sticky Disease.$h[electronic resource] 260 $c2016 520 $aPapaya sticky disease, or ?meleira?, is one of the major diseases of papaya in Brazil and Mexico, capable of causing complete crop loss. The causal agent of sticky disease was identified as an isometric virus with a double stranded RNA (dsRNA) genome, named papaya meleira virus (PMeV). In the present study, PMeV dsRNA and a second RNA band of approximately 4.5 kb, both isolated from latex of papaya plants with severe symptoms of sticky disease, were deep-sequenced. The nearly complete sequence obtained for PMeV dsRNA is 8,814 nucleotides long and contains two putative ORFs; the predicted ORF1 and ORF2 display similarity to capsid proteins and RdRp's, respectively, from mycoviruses tentatively classified in the family Totiviridae. The sequence obtained for the second RNA is 4,515 nucleotides long and contains two putative ORFs. The predicted ORFs 1 and 2 display 48% and 73% sequence identity, respectively, with the corresponding proteins of papaya virus Q, an umbravirus recently described infecting papaya in Ecuador. Viral purification in a sucrose gradient allowed separation of particles containing each RNA. Mass spectrometry analysis indicated that both PMeV and the second RNA virus (named papaya meleira virus 2, PMeV2) were encapsidated in particles formed by the protein encoded by PMeV ORF1. The presence of both PMeV and PMeV2 was confirmed in field plants showing typical symptoms of sticky disease. Interestingly, PMeV was detected alone in asymptomatic plants. Together, our results indicate that sticky disease is associated with double infection by PMeV and PMeV2. 653 $aMamão 653 $aMamoeiro 653 $aMeleira 653 $aPapaya Meleira Virus 700 1 $aAMARAL, R. J. V. 700 1 $aVENTURA, J. A. 700 1 $aGODINHO, M. T. 700 1 $aAMARAL, J. G. 700 1 $aSOUZA, F. O. 700 1 $aZERBINI, P. A. 700 1 $aFERNANDES, P. M. B. 773 $tPLoS ONE$gv. 11, n. 5, e0155240.
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Biblioteca Rui Tendinha (BRT) |
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Registro Completo |
Biblioteca(s): |
Biblioteca Rui Tendinha. |
Data corrente: |
31/10/2016 |
Data da última atualização: |
31/10/2016 |
Tipo da produção científica: |
Publicação em Anais de Congresso |
Autoria: |
NUNES, J. R.; OLIVEIRA, F. de T. G. de.; VITÓRIA, R. Z. da.; ARAÚJO, E. L.; VALFRÉ, P. P.; POSSE, S. C. P.; DOUSSEAU, S.; ARANTES, L. de O.; CERRI NETO, B.; SANTOS, M. F. dos.; CRASQUE, J. |
Afiliação: |
Joyce Ribeiro Nunes, Incaper/Bolsista Faculdade Pitágoras de Linhares; Felipe de Tássio Gonçalves de Oliveira, CEUNES; Rafael Zucateli da Vitória, CEUNES; Edlaine Lacerda Araújo, Incaper/Bolsista Faculdade Pitágoras de Linhares; Poliana Pratti Valfré, Incaper/Bolsista Embrapa Café; Sheila Cristina Prucoli Posse, Incaper; Sara Dousseau Arantes, Incaper; Lucio de Oliveira Arantes, Incaper; Basilio Cerri Neto, Incaper/Estágio; Mikaelle Franco dos Santos, Incaper/Bolsista Faculdade Pitágoras de Linhares; Jeane Crasque, Incaper/Bolsista Faculdade Pitágoras de Linhares. |
Título: |
Qualidade fisiológica de semetes de aroeira em função da maturação dos frutos sob diferentes temperaturas de germinação |
Ano de publicação: |
2016 |
Fonte/Imprenta: |
In: CONGRESSO NACIONAL DE BOTÂNICA, 67., 2016, Vitória, ES. Conectando diversidades, revelando o desconhecido: resumos. Brasília, DF: Sociedade Botânica do Brasil. |
Idioma: |
Português |
Notas: |
Resumo. |
Palavras-Chave: |
Aroeira; CNBOT; Maturação; Qualidade fisiológica; Schinus terebinthifolius Raddi; Temperatura. |
Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
URL: |
http://biblioteca.incaper.es.gov.br/digital/bitstream/item/2398/1/BRT-qualidadefisiologicadesementesdearoeiraemfuncaodamaturacaodosfrutos-nunes.pdf
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Marc: |
LEADER 01048nam a2200301 a 4500 001 1012904 005 2016-10-31 008 2016 bl uuuu u01u1 u #d 100 1 $aNUNES, J. R. 245 $aQualidade fisiológica de semetes de aroeira em função da maturação dos frutos sob diferentes temperaturas de germinação$h[electronic resource] 260 $aIn: CONGRESSO NACIONAL DE BOTÂNICA, 67., 2016, Vitória, ES. Conectando diversidades, revelando o desconhecido: resumos. Brasília, DF: Sociedade Botânica do Brasil.$c2016 500 $aResumo. 653 $aAroeira 653 $aCNBOT 653 $aMaturação 653 $aQualidade fisiológica 653 $aSchinus terebinthifolius Raddi 653 $aTemperatura 700 1 $aOLIVEIRA, F. de T. G. de. 700 1 $aVITÓRIA, R. Z. da. 700 1 $aARAÚJO, E. L. 700 1 $aVALFRÉ, P. P. 700 1 $aPOSSE, S. C. P. 700 1 $aDOUSSEAU, S. 700 1 $aARANTES, L. de O. 700 1 $aCERRI NETO, B. 700 1 $aSANTOS, M. F. dos. 700 1 $aCRASQUE, J.
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