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Registro Completo |
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Biblioteca(s): |
Biblioteca Rui Tendinha. |
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Data corrente: |
26/05/2023 |
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Data da última atualização: |
26/05/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
BATISTA, R. S.; COSTA, H.; PEREIRA, L. A.; BERNARDES, C. de O.; ABREU, K. P. de; MENINI, L. |
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Afiliação: |
Ringo Souza Batista, Ifes Alegre; Helcio Costa, Incaper; Luciana Alves Parreira, UFES; Carolina de Oliveira Bernardes, Ifes Alegre; Karla Maria Pedra de Abreu, Ifes Alegre; Luciano Menini, Ifes Alegre. |
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Título: |
Essential oil of Piper macedoi Yunck. leaves, potential alternative for the management of banana anthracnose disease |
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Ano de publicação: |
2023 |
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Fonte/Imprenta: |
Journal of Natural Pesticide Research, v.5, p. 1-7, 2023. |
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Idioma: |
Inglês |
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Conteúdo: |
The fungus Colletotrichum musae could cause a disease called anthracnose which reduces the quality of the banana fruit in the postharvest period. Anthracnose causes rots, peel lesions and generate great economic losses. Synthetic fungicides, used as an attempt to reduce the pathogen population, although effective, when used in amounts and frequency above the recommended, cause damage the environment and human health and can induce resistance. Research has focused in the search for alternative methods to reduce the use of synthetic substances in managing of agricultural diseases. The control of pathogens using compounds of natural origin is highlighted among new management techniques. Thus, we aimed to extract and characterize the essential oil from Piper macedoi leaves and evaluate its effect in the inhibiting the mycelial growth of the fungus C. musae in vitro. The essential oil was extracted by hydrodistillation, analyzed and characterized by Gas Chromatography and submitted to assays of fungicidal activity with C. musae. In comparison with dry plant material, yield of P. macedoi essential oil was 0.93 %. Piperitone (26.48 %), silvestrene (13.03 %), and bicyclogermacrene (10.45 %), were the major compounds identified in the essential oil composition considering the percentage in area. Regarding the antifungal activity tests, the essential oil showed a low inhibitory concentration (IC) for the IC50 (0.804 mL.L−1) and IC90 (1.80 mL.L−1), showing that the essential oil from P. macedoi leaves has great potential to control C. musae. MenosThe fungus Colletotrichum musae could cause a disease called anthracnose which reduces the quality of the banana fruit in the postharvest period. Anthracnose causes rots, peel lesions and generate great economic losses. Synthetic fungicides, used as an attempt to reduce the pathogen population, although effective, when used in amounts and frequency above the recommended, cause damage the environment and human health and can induce resistance. Research has focused in the search for alternative methods to reduce the use of synthetic substances in managing of agricultural diseases. The control of pathogens using compounds of natural origin is highlighted among new management techniques. Thus, we aimed to extract and characterize the essential oil from Piper macedoi leaves and evaluate its effect in the inhibiting the mycelial growth of the fungus C. musae in vitro. The essential oil was extracted by hydrodistillation, analyzed and characterized by Gas Chromatography and submitted to assays of fungicidal activity with C. musae. In comparison with dry plant material, yield of P. macedoi essential oil was 0.93 %. Piperitone (26.48 %), silvestrene (13.03 %), and bicyclogermacrene (10.45 %), were the major compounds identified in the essential oil composition considering the percentage in area. Regarding the antifungal activity tests, the essential oil showed a low inhibitory concentration (IC) for the IC50 (0.804 mL.L−1) and IC90 (1.80 mL.L−1), showing that the essentia... Mostrar Tudo |
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Palavras-Chave: |
Piper macedoi Yunck. |
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Thesagro: |
Antracnose; Banana; Doença; Doença de Planta; Fungicida; Fungicida Orgânico; Fungo; Óleo Essencial. |
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Thesaurus NAL: |
Colletotrichum musae. |
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Categoria do assunto: |
-- |
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URL: |
https://biblioteca.incaper.es.gov.br/digital/bitstream/item/4440/1/Essential-oil-of-Piper-macedoi-Yunck.pdf
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Marc: |
LEADER 02438naa a2200301 a 4500
001 1024920
005 2023-05-26
008 2023 bl uuuu u00u1 u #d
100 1 $aBATISTA, R. S.
245 $aEssential oil of Piper macedoi Yunck. leaves, potential alternative for the management of banana anthracnose disease$h[electronic resource]
260 $c2023
520 $aThe fungus Colletotrichum musae could cause a disease called anthracnose which reduces the quality of the banana fruit in the postharvest period. Anthracnose causes rots, peel lesions and generate great economic losses. Synthetic fungicides, used as an attempt to reduce the pathogen population, although effective, when used in amounts and frequency above the recommended, cause damage the environment and human health and can induce resistance. Research has focused in the search for alternative methods to reduce the use of synthetic substances in managing of agricultural diseases. The control of pathogens using compounds of natural origin is highlighted among new management techniques. Thus, we aimed to extract and characterize the essential oil from Piper macedoi leaves and evaluate its effect in the inhibiting the mycelial growth of the fungus C. musae in vitro. The essential oil was extracted by hydrodistillation, analyzed and characterized by Gas Chromatography and submitted to assays of fungicidal activity with C. musae. In comparison with dry plant material, yield of P. macedoi essential oil was 0.93 %. Piperitone (26.48 %), silvestrene (13.03 %), and bicyclogermacrene (10.45 %), were the major compounds identified in the essential oil composition considering the percentage in area. Regarding the antifungal activity tests, the essential oil showed a low inhibitory concentration (IC) for the IC50 (0.804 mL.L−1) and IC90 (1.80 mL.L−1), showing that the essential oil from P. macedoi leaves has great potential to control C. musae.
650 $aColletotrichum musae
650 $aAntracnose
650 $aBanana
650 $aDoença
650 $aDoença de Planta
650 $aFungicida
650 $aFungicida Orgânico
650 $aFungo
650 $aÓleo Essencial
653 $aPiper macedoi Yunck
700 1 $aCOSTA, H.
700 1 $aPEREIRA, L. A.
700 1 $aBERNARDES, C. de O.
700 1 $aABREU, K. P. de
700 1 $aMENINI, L.
773 $tJournal of Natural Pesticide Research$gv.5, p. 1-7, 2023.
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Registro original: |
Biblioteca Rui Tendinha (BRT) |
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Biblioteca |
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Origem |
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Classificação |
Cutter |
Registro |
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Status |
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Registro Completo |
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Biblioteca(s): |
Biblioteca Rui Tendinha. |
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Data corrente: |
30/12/2014 |
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Data da última atualização: |
01/09/2015 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
COSTA, H. B.; FERNANDES, P. M. B.; ROMÃO, W.; VENTURA, J. A. |
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Afiliação: |
Helber B. Costa; Patricia M.B. Fernandes; Wanderson Romão; Jose Aires Ventura, Incaper. |
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Título: |
A new procedure based on column chromatography to purify bromelain by ion exchange plus gel filtration chromatographies. |
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Ano de publicação: |
2014 |
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Fonte/Imprenta: |
Industrial Crops and Products, Vol. 59, p. 163-168, august 2014. |
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Páginas: |
6 p. |
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Idioma: |
Inglês |
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Conteúdo: |
Bioproducts separation and purification processes are an important segment of the biotechnical industry. Bromelain is an enzyme which has great commercial value and is of wide interest in the pharmaceutical, alimentary, and textile industries, among others. The goal of this study was to develop a new method for bromelain purification from the stem residues resulting from agricultural processing of the pineapple plant. Bromelain was purified using two liquid chromatography steps, ion exchange plus gel filtration chromatography. Use of the methodology which was developed produced an enzyme with a molecular weight of 30 kDa (confirmed by SDS-PAGE), high recovery of enzymatic activity (89%), and with a purification factor of 16.93, a result superior to the methodologies described in the literature. HPLC showed the presence of two peaks in the ion exchange chromatogram and only one protein in the gel filtration chromatogram. Results indicate that, depending on the destination of the bromelain, the process can be stopped after the first purification step. The MALDI-TOF MS provided the peptide mass fingerprint of bromelain and MALDI-MS/MS the fragmentation profile and sequencing of the ions of m/z 951 and 1584. Thus, the connectivity and chemical structure of bromelain was confirmed. Moreover, besides its superiority to other methodologies, it can be applied to take advantage of the agricultural and industrial pineapple plant residues. |
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Thesaurus NAL: |
Bromelain; Enzyme; Forbromelain; Pineapple; Purification. |
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Categoria do assunto: |
-- |
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URL: |
http://biblioteca.incaper.es.gov.br/digital/bitstream/item/419/1/New-Procedure-Chromatography-to-Purify-Bromelain-2014.pdf
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Marc: |
LEADER 02130naa a2200229 a 4500
001 1004754
005 2015-09-01
008 2014 bl uuuu u00u1 u #d
100 1 $aCOSTA, H. B.
245 $aA new procedure based on column chromatography to purify bromelain by ion exchange plus gel filtration chromatographies.$h[electronic resource]
260 $c2014
300 $a6 p.
520 $aBioproducts separation and purification processes are an important segment of the biotechnical industry. Bromelain is an enzyme which has great commercial value and is of wide interest in the pharmaceutical, alimentary, and textile industries, among others. The goal of this study was to develop a new method for bromelain purification from the stem residues resulting from agricultural processing of the pineapple plant. Bromelain was purified using two liquid chromatography steps, ion exchange plus gel filtration chromatography. Use of the methodology which was developed produced an enzyme with a molecular weight of 30 kDa (confirmed by SDS-PAGE), high recovery of enzymatic activity (89%), and with a purification factor of 16.93, a result superior to the methodologies described in the literature. HPLC showed the presence of two peaks in the ion exchange chromatogram and only one protein in the gel filtration chromatogram. Results indicate that, depending on the destination of the bromelain, the process can be stopped after the first purification step. The MALDI-TOF MS provided the peptide mass fingerprint of bromelain and MALDI-MS/MS the fragmentation profile and sequencing of the ions of m/z 951 and 1584. Thus, the connectivity and chemical structure of bromelain was confirmed. Moreover, besides its superiority to other methodologies, it can be applied to take advantage of the agricultural and industrial pineapple plant residues.
650 $aBromelain
650 $aEnzyme
650 $aForbromelain
650 $aPineapple
650 $aPurification
700 1 $aFERNANDES, P. M. B.
700 1 $aROMÃO, W.
700 1 $aVENTURA, J. A.
773 $tIndustrial Crops and Products, Vol. 59, p. 163-168, august 2014.
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