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| 3. |  | COSTA, A. L.; CUQUETTO, C. R.; HOLANDA, P. E.; PERINNI, J. L.; RANGEL, O. J. P. Chamadas públicas de Ater: estudo de caso envolvendo o municÃpio de São Gabriel da Palha/ES. In: SIMPÓSIO INCAPER PESQUISA, 3. , Vitória, ES. Chamadas públicas de Ater: estudo de caso envolvendo o municÃpio de São Gabriel da Palha/ES. Editores, Andréa Ferreira da Costa, Marlon Dutra Degli Esposti e Renato Corrêa Taques. Vitória, ES : Incaper, p. 68, 2024. p. 68| Biblioteca(s): Biblioteca Rui Tendinha. |
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| 6. | | RODRIGUES, D. D.; MARIANO, S. R.; SILVA, M. A. B. da; RANGEL, O. J. P.; CARVALHO, C. S.; TRUGILHO, G. A.; SILVA, M. A. P. da; GONÇALVES, M. M.; EGIDIO, L. S.; XAVIER, S. A. B.; CASSA, N.; CRESPO, A. M.; LOUBACK, G. C.; PERON, I. B.; SOUZA, M. N. Estratégias de recuperação de áreas degradadas. In: SOUZA, M. N. (Org.) Tópicos em recuperação de áreas degradadas. Vol. VI. Canoas, RS: Mérida Publishers, cap. 3, p. 94-125, 2023.| Biblioteca(s): Biblioteca Rui Tendinha. |
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| 8. | | CRESPO, A. M.; GONÇALVES, D. da C.; SOUZA, M. N.; ZANÚNCIO JUNIOR, J. S.; COSTA, H.; FAVARATO, L. F.; RANGEL, O. J. P.; ARAÚJO, J. B. S. Manejo da lagarta-do-cartucho do milho (Spodoptera frugiperda): panorama geral das atualizações no controle alternativo. Alegre, ES : IFES Alegre, 2021. 20 p. (IFES Alegre. Boletim Técnico, 06).| Biblioteca(s): Biblioteca Rui Tendinha. |
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| 9. | | SOUZA, G. S.; THIENGO, C. C.; SILVA, M. W. da; FONSECA, A. L. C. da; GUIMARÃES, L. A. de O. P.; RANGEL, O. J. P.; BURAK, D. L. Sistema silvipastoril e semeadura direta na renovação de pastagem degrada na Bacia do Rio Itapemirim. In: CONGRESSO CAPIXABA DE PESQUISA AGROPECUÁRIA, 1., Vitória, ES. Anais 2021 : congresso capixaba de pesquisa agropecuária [recurso eletrônico]. Vitória, ES: Incaper, 2021. color. PDF ; 25,4 MB.
E-book, no formato PDF. (Incaper, Documentos, 289). Pedro Luís Pereira Teixeira de Carvalho, Carlos Henrique Rodrigues de Oliveira, José Aires Ventura, Marcos Vinicius Winckler Caldeira e Romário Gava Ferrão, editores. p. 89| Biblioteca(s): Biblioteca Rui Tendinha. |
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| 10. | | SILVA, J. M. V. de O. da; SOUZA, M. N.; RANGEL, O. J. P.; FORNAZIER, M. L.; LOUBACK, G. C.; PIROVANI, G.; CAON, B. L.; MOREIRA, M. F.; SIQUEIRA, C. B.; TRUGILHO, G. A.; KAULZ, M.; CRESPO, A. M.; GOMES, A. L. C.; GALL, M. V. C.; PINHEIRO, A. C. M.; GUERRA, A. C. M.; PERON, I. B. Sistemas agroflorestais e consórcios na cultura do café. In: SOUZA, Mauricio Novaes (Org.). Tópicos em recuperação de áreas degradadas, v. 4. Canoas, RS: Méricadas Publishers, 2022.| Biblioteca(s): Biblioteca Rui Tendinha. |
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| 11. | | CRESPO, A. M.; SOUZA, M. N.; FAVARATO, L. F.; GUARÇONI, R. G.; ARAÚJO, J. B. S.; RANGEL, O. J. P.; SOUZA, J. L. de. Survey of the floristic composition and the structure of spontaneous vegetation present at green corn cultivated in organic no-tillage system. IJAERS, v. 7, n. 11, p. 184-193, nov. 2020.| Biblioteca(s): Biblioteca Rui Tendinha. |
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| 12. | | CRESPO, A. M.; SOUZA, M. N.; FAVARATO, L. F.; GUARÇONI, R. G.; ARAÚJO, J. B. S.; RANGEL, O. J. P.; SOUZA, J. L. de.; GONÇALVES, D. da C. The green corn development and yield on different summer soil covering plants in the organic no-tillage system. International Journal of Advanced Engineering Research and Science, v. 9, p. 3, 2022.| Biblioteca(s): Biblioteca Rui Tendinha. |
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Biblioteca(s): |
Biblioteca Rui Tendinha. |
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Data corrente: |
13/01/2015 |
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Data da última atualização: |
13/01/2015 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
MARRACCINI, P.; VINECKY, F.; ALVES, G. S. C.; RAMOS, H. J. O.; ELBELT, S.; VIEIRA, N. G.; CARNEIRO, F. A.; SUJII, P. S.; ALEKCEVETCH, J. C.; SILVA, V. A.; DaMATTA, F. M.; FERRÃO, M. A. G.; LEROY, T.; POT, D.; VIEIRA, L. G. E.; SILVA, F. R. da; ANDRADE, A. C. |
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Afiliação: |
Maria Amélia Gava Ferrão, Incaper/Embrapa Café. |
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Título: |
Differentially expressed genes and proteins upon drought acclimation in tolerant and sensitive genotypes of Coffea canephora. |
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Ano de publicação: |
2012 |
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Fonte/Imprenta: |
Journal of Experimental Botany, Oxford v. 63, n. 11, p. 4191-4212, 2012. |
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Idioma: |
Inglês |
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Conteúdo: |
The aim of this study was to investigate the molecular mechanisms underlying drought acclimation in coffee plants by the identification of candidate genes (CGs) using different approaches. The first approach used the data generated during the Brazilian Coffee expressed sequence tag (EST) project to select 13 CGs by an in silico analysis (electronic northern). The second approach was based on screening macroarrays spotted with plasmid DNA (coffee ESTs) with separate hybridizations using leaf cDNA probes from drought-tolerant and susceptible clones of Coffea canephora var. Conilon, grown under different water regimes. This allowed the isolation of seven additional CGs. The third approach used two-dimensional gel electrophoresis to identify proteins displaying differential accumulation in leaves of drought-tolerant and susceptible clones of C. canephora. Six of them were characterized by MALDI-TOF-MS/MS (matrix-assisted laser desorption-time of flight-tandem mass spectrometry) and the corresponding proteins were identified. Finally, additional CGs were selected from the literature, and quantitative real-time polymerase chain reaction (qPCR) was performed to analyse the expression of all identified CGs. Altogether, >40 genes presenting differential gene expression during drought acclimation were identified, some of them showing different expression profiles between drought-tolerant and susceptible clones. Based on the obtained results, it can be concluded that factors involved a complex network of responses probably involving the abscisic signalling pathway and nitric oxide are major molecular determinants that might explain the better efficiency in controlling stomata closure and transpiration displayed by drought-tolerant clones of C. canephora. MenosThe aim of this study was to investigate the molecular mechanisms underlying drought acclimation in coffee plants by the identification of candidate genes (CGs) using different approaches. The first approach used the data generated during the Brazilian Coffee expressed sequence tag (EST) project to select 13 CGs by an in silico analysis (electronic northern). The second approach was based on screening macroarrays spotted with plasmid DNA (coffee ESTs) with separate hybridizations using leaf cDNA probes from drought-tolerant and susceptible clones of Coffea canephora var. Conilon, grown under different water regimes. This allowed the isolation of seven additional CGs. The third approach used two-dimensional gel electrophoresis to identify proteins displaying differential accumulation in leaves of drought-tolerant and susceptible clones of C. canephora. Six of them were characterized by MALDI-TOF-MS/MS (matrix-assisted laser desorption-time of flight-tandem mass spectrometry) and the corresponding proteins were identified. Finally, additional CGs were selected from the literature, and quantitative real-time polymerase chain reaction (qPCR) was performed to analyse the expression of all identified CGs. Altogether, >40 genes presenting differential gene expression during drought acclimation were identified, some of them showing different expression profiles between drought-tolerant and susceptible clones. Based on the obtained results, it can be concluded that factors involved a... Mostrar Tudo |
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Palavras-Chave: |
Candidate gene; Coffea canephora; Differential expression; Drought acclimation; Genética; Proteomic; Proteômica; Real time PCR. |
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Thesagro: |
Café; Coffea canephora; Genetics. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 03034naa a2200445 a 4500
001 1004907
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100 1 $aMARRACCINI, P.
245 $aDifferentially expressed genes and proteins upon drought acclimation in tolerant and sensitive genotypes of Coffea canephora.$h[electronic resource]
260 $c2012
520 $aThe aim of this study was to investigate the molecular mechanisms underlying drought acclimation in coffee plants by the identification of candidate genes (CGs) using different approaches. The first approach used the data generated during the Brazilian Coffee expressed sequence tag (EST) project to select 13 CGs by an in silico analysis (electronic northern). The second approach was based on screening macroarrays spotted with plasmid DNA (coffee ESTs) with separate hybridizations using leaf cDNA probes from drought-tolerant and susceptible clones of Coffea canephora var. Conilon, grown under different water regimes. This allowed the isolation of seven additional CGs. The third approach used two-dimensional gel electrophoresis to identify proteins displaying differential accumulation in leaves of drought-tolerant and susceptible clones of C. canephora. Six of them were characterized by MALDI-TOF-MS/MS (matrix-assisted laser desorption-time of flight-tandem mass spectrometry) and the corresponding proteins were identified. Finally, additional CGs were selected from the literature, and quantitative real-time polymerase chain reaction (qPCR) was performed to analyse the expression of all identified CGs. Altogether, >40 genes presenting differential gene expression during drought acclimation were identified, some of them showing different expression profiles between drought-tolerant and susceptible clones. Based on the obtained results, it can be concluded that factors involved a complex network of responses probably involving the abscisic signalling pathway and nitric oxide are major molecular determinants that might explain the better efficiency in controlling stomata closure and transpiration displayed by drought-tolerant clones of C. canephora.
650 $aCafé
650 $aCoffea canephora
650 $aGenetics
653 $aCandidate gene
653 $aCoffea canephora
653 $aDifferential expression
653 $aDrought acclimation
653 $aGenética
653 $aProteomic
653 $aProteômica
653 $aReal time PCR
700 1 $aVINECKY, F.
700 1 $aALVES, G. S. C.
700 1 $aRAMOS, H. J. O.
700 1 $aELBELT, S.
700 1 $aVIEIRA, N. G.
700 1 $aCARNEIRO, F. A.
700 1 $aSUJII, P. S.
700 1 $aALEKCEVETCH, J. C.
700 1 $aSILVA, V. A.
700 1 $aDaMATTA, F. M.
700 1 $aFERRÃO, M. A. G.
700 1 $aLEROY, T.
700 1 $aPOT, D.
700 1 $aVIEIRA, L. G. E.
700 1 $aSILVA, F. R. da
700 1 $aANDRADE, A. C.
773 $tJournal of Experimental Botany, Oxford$gv. 63, n. 11, p. 4191-4212, 2012.
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