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| 1. |  | BARKER, D. L.; DOUSSEAU, S.; SCHMILDT, E. R.; VENTURA, J. A.; ARANTES, L. de O.; SILVA, J.F. da; CERRI NETO, B.; BUFFON, S. B.; FONTES, P. S. F. Performance of 'Vitória' pineapple in response to different types of shoots and ages of floral induction. Pesquisa Agropecuária Brasileira, Brasília, v. 55, e01598, 2020.| Biblioteca(s): Biblioteca Rui Tendinha. |
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| 2. | | COSTA, J. C.; CORREIA, L. Z.; SILVA, J. F. da.; LIMA, K. C. C.; SANT'ANA, C.; SILVA, F. R. N. e.; SOUZA, G. A. R. de.; FERREIRA, T. R.; OLIVEIRA, D. C.; ARANTES, L. de O.; DOUSSEAU, S.; SANTANA, E. N. de. Influência do ácido acético na manutenção da qualidade do fruto de mamão 'golden' pós-colheita. In: ENCONTRO LATINO-AMERICANO DE INICIAÇÃO CIENTÍFICA, 22.; ENCONTRO LATINO AMERICANO DE PÓS GRADUAÇÃO, 18.; ENCONTRO NACIONAL DE INICIAÇÃO À DOCÊNCIA, 8., 2017, Urbanova .Patrimônio e Cultura Desafios da Ciência Frente a Identidades Plurais. São José dos Campos: UNIVAP, 2018. 5 p.| Biblioteca(s): Biblioteca Rui Tendinha. |
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| 3. | | DOUSSEAU, S.; MACHADO FILHO, J. A.; LAVANHOLE, D. F.; BRITO, A. M. S. de.; RODRIGUES, P de S.; KLEM, D. L. B.; CORREIA, L. Z.; SILVA, J. F. da.; SANT'ANA C.; ARANTES, L. de O. Cuidados na colheita e pós-colheita de café, de frutíferas e de hortaliças para a garantia de alimentos seguros. Incaper Em Revista, Vitória, v. 8, p. 6-16, jan/dez 2017.| Biblioteca(s): Biblioteca Rui Tendinha. |
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| 4. | | FAVARATO, L. F.; DOUSSEAU, S.; BUFFON, S. B.; BARKER, D. L.; SILVA, J. F. da.; OLIVEIRA, F. de T. G. de.; ARANTES, L. de O.; CALATRONI, D. Avaliação do crescimento inicial de diferentes pesos e mudas do abacaxizeiro cv. 'Vitória'. In: SEMINÁRIO DE INICIAÇÃO CIENTÍFICA E TECNOLÓGICA (SICT) DO INCAPER, 1., 2016. JORNADA DE INICIAÇÃO CIENTÍFICA, DESENVOLVIMENTO TECNOLÓGICO E INOVAÇÃO DO IFES, 11., 2016. Venda Nova do Imigrante, ES : IFES; Incaper, 2016.| Biblioteca(s): Biblioteca Rui Tendinha. |
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| 7. | | KÜSTER, I. S.; SILVA, J, F, da.; SCHIMILD, E. R.; ALEXANDRE, R. S.; DOUSSEAU, S. Fenologia do abacaxizeiro cv. Vitória submetido em diferentes épocas de plantio e indução floral. CONGRESSO BRASILEIRO DE FRUTICULTURA, 24., 2016, São Luís. Fruteiras Nativas e Sustentabilidade : anais. São Luís : Sociedade Brasileira de Fruticultura, 2016.| Biblioteca(s): Biblioteca Rui Tendinha. |
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| 9. | | SANT'ANA, R. C.de.; BUFFON, S. B.; SANTOS, L. C.; HADDADE, I. R.; BARKER, D. L.; FAVARATO, F.; SILVA, J. F. da.; MOREIRA, M.; VENTURINI, M. T.; DOUSSEAU, S. Avaliação morfológica de acessos de mandioca da região Norte do Estado do Espírito Santo. REVISTA UNIVAP, São José dos Campos, v. 22, n. 40, p. 46, 2016. Número especial da Revista UNIVAP Online, com o resumo de todos os artigos os melhores artigos de cada área do XX Encontro de Iniciação Científica, XVI Encontro de Pós-Graduação, X INIC Jr da UNIVAP, VI INID.| Biblioteca(s): Biblioteca Rui Tendinha. |
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| 13. | | SILVA, J. F. da.; DOUSSEAU, S.; BARKER, D. L.; BUFFON, S. B.; CALATRONI, D.; FAVARATO, L. F.; OLIVEIRA, F. de T. G. de.; ARANTES, L. de O. Desenvolvimento vegetativo, produção e qualidade de frutos do abacaxizeiro cv. Vitória em função do tipo de mudas. In: SEMINÁRIO DE INICIAÇÃO CIENTÍFICA E TECNOLÓGICA (SICT) DO INCAPER, 1., 2016. JORNADA DE INICIAÇÃO CIENTÍFICA, DESENVOLVIMENTO TECNOLÓGICO E INOVAÇÃO DO IFES, 11., 2016. Venda Nova do Imigrante, ES : IFES; Incaper, 2016.| Biblioteca(s): Biblioteca Rui Tendinha. |
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| 15. | | SOUZA, G. A. R. de; SILVA, F. R. N. e; COSTA, R. J.; FERREIRA, T. R.; SILVA, J. F. da.; CORREIA, L. Z.; LIMA, K. C. C.; SANT'ANA, C.; DOUSSEAU, S.; ARANTES, L. de O.; SANTANA, E. N. de.; SCALZER, A. P. Acidificação de frutos de mamão em solução de ácido acético e sua influência sobre ps caracteres físico-químicos dos frutos. In: ENCONTRO LATINO-AMERICANO DE INICIAÇÃO CIENTÍFICA, 22.; ENCONTRO LATINO AMERICANO DE PÓS GRADUAÇÃO, 18.; ENCONTRO NACIONAL DE INICIAÇÃO À DOCÊNCIA, 8., 2017, Urbanova .Patrimônio e Cultura Desafios da Ciência Frente a Identidades Plurais. São José dos Campos: UNIVAP, 2018.| Biblioteca(s): Biblioteca Rui Tendinha. |
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| Registros recuperados : 15 | |
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 | Acesso ao texto completo restrito à biblioteca da Biblioteca Rui Tendinha. Para informações adicionais entre em contato com biblioteca@incaper.es.gov.br. |
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Registro Completo |
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Biblioteca(s): |
Biblioteca Rui Tendinha. |
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Data corrente: |
13/01/2015 |
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Data da última atualização: |
13/01/2015 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
MARRACCINI, P.; VINECKY, F.; ALVES, G. S. C.; RAMOS, H. J. O.; ELBELT, S.; VIEIRA, N. G.; CARNEIRO, F. A.; SUJII, P. S.; ALEKCEVETCH, J. C.; SILVA, V. A.; DaMATTA, F. M.; FERRÃO, M. A. G.; LEROY, T.; POT, D.; VIEIRA, L. G. E.; SILVA, F. R. da; ANDRADE, A. C. |
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Afiliação: |
Maria Amélia Gava Ferrão, Incaper/Embrapa Café. |
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Título: |
Differentially expressed genes and proteins upon drought acclimation in tolerant and sensitive genotypes of Coffea canephora. |
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Ano de publicação: |
2012 |
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Fonte/Imprenta: |
Journal of Experimental Botany, Oxford v. 63, n. 11, p. 4191-4212, 2012. |
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Idioma: |
Inglês |
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Conteúdo: |
The aim of this study was to investigate the molecular mechanisms underlying drought acclimation in coffee plants by the identification of candidate genes (CGs) using different approaches. The first approach used the data generated during the Brazilian Coffee expressed sequence tag (EST) project to select 13 CGs by an in silico analysis (electronic northern). The second approach was based on screening macroarrays spotted with plasmid DNA (coffee ESTs) with separate hybridizations using leaf cDNA probes from drought-tolerant and susceptible clones of Coffea canephora var. Conilon, grown under different water regimes. This allowed the isolation of seven additional CGs. The third approach used two-dimensional gel electrophoresis to identify proteins displaying differential accumulation in leaves of drought-tolerant and susceptible clones of C. canephora. Six of them were characterized by MALDI-TOF-MS/MS (matrix-assisted laser desorption-time of flight-tandem mass spectrometry) and the corresponding proteins were identified. Finally, additional CGs were selected from the literature, and quantitative real-time polymerase chain reaction (qPCR) was performed to analyse the expression of all identified CGs. Altogether, >40 genes presenting differential gene expression during drought acclimation were identified, some of them showing different expression profiles between drought-tolerant and susceptible clones. Based on the obtained results, it can be concluded that factors involved a complex network of responses probably involving the abscisic signalling pathway and nitric oxide are major molecular determinants that might explain the better efficiency in controlling stomata closure and transpiration displayed by drought-tolerant clones of C. canephora. MenosThe aim of this study was to investigate the molecular mechanisms underlying drought acclimation in coffee plants by the identification of candidate genes (CGs) using different approaches. The first approach used the data generated during the Brazilian Coffee expressed sequence tag (EST) project to select 13 CGs by an in silico analysis (electronic northern). The second approach was based on screening macroarrays spotted with plasmid DNA (coffee ESTs) with separate hybridizations using leaf cDNA probes from drought-tolerant and susceptible clones of Coffea canephora var. Conilon, grown under different water regimes. This allowed the isolation of seven additional CGs. The third approach used two-dimensional gel electrophoresis to identify proteins displaying differential accumulation in leaves of drought-tolerant and susceptible clones of C. canephora. Six of them were characterized by MALDI-TOF-MS/MS (matrix-assisted laser desorption-time of flight-tandem mass spectrometry) and the corresponding proteins were identified. Finally, additional CGs were selected from the literature, and quantitative real-time polymerase chain reaction (qPCR) was performed to analyse the expression of all identified CGs. Altogether, >40 genes presenting differential gene expression during drought acclimation were identified, some of them showing different expression profiles between drought-tolerant and susceptible clones. Based on the obtained results, it can be concluded that factors involved a... Mostrar Tudo |
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Palavras-Chave: |
Candidate gene; Coffea canephora; Differential expression; Drought acclimation; Genética; Proteomic; Proteômica; Real time PCR. |
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Thesagro: |
Café; Coffea canephora; Genetics. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 03034naa a2200445 a 4500
001 1004907
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100 1 $aMARRACCINI, P.
245 $aDifferentially expressed genes and proteins upon drought acclimation in tolerant and sensitive genotypes of Coffea canephora.$h[electronic resource]
260 $c2012
520 $aThe aim of this study was to investigate the molecular mechanisms underlying drought acclimation in coffee plants by the identification of candidate genes (CGs) using different approaches. The first approach used the data generated during the Brazilian Coffee expressed sequence tag (EST) project to select 13 CGs by an in silico analysis (electronic northern). The second approach was based on screening macroarrays spotted with plasmid DNA (coffee ESTs) with separate hybridizations using leaf cDNA probes from drought-tolerant and susceptible clones of Coffea canephora var. Conilon, grown under different water regimes. This allowed the isolation of seven additional CGs. The third approach used two-dimensional gel electrophoresis to identify proteins displaying differential accumulation in leaves of drought-tolerant and susceptible clones of C. canephora. Six of them were characterized by MALDI-TOF-MS/MS (matrix-assisted laser desorption-time of flight-tandem mass spectrometry) and the corresponding proteins were identified. Finally, additional CGs were selected from the literature, and quantitative real-time polymerase chain reaction (qPCR) was performed to analyse the expression of all identified CGs. Altogether, >40 genes presenting differential gene expression during drought acclimation were identified, some of them showing different expression profiles between drought-tolerant and susceptible clones. Based on the obtained results, it can be concluded that factors involved a complex network of responses probably involving the abscisic signalling pathway and nitric oxide are major molecular determinants that might explain the better efficiency in controlling stomata closure and transpiration displayed by drought-tolerant clones of C. canephora.
650 $aCafé
650 $aCoffea canephora
650 $aGenetics
653 $aCandidate gene
653 $aCoffea canephora
653 $aDifferential expression
653 $aDrought acclimation
653 $aGenética
653 $aProteomic
653 $aProteômica
653 $aReal time PCR
700 1 $aVINECKY, F.
700 1 $aALVES, G. S. C.
700 1 $aRAMOS, H. J. O.
700 1 $aELBELT, S.
700 1 $aVIEIRA, N. G.
700 1 $aCARNEIRO, F. A.
700 1 $aSUJII, P. S.
700 1 $aALEKCEVETCH, J. C.
700 1 $aSILVA, V. A.
700 1 $aDaMATTA, F. M.
700 1 $aFERRÃO, M. A. G.
700 1 $aLEROY, T.
700 1 $aPOT, D.
700 1 $aVIEIRA, L. G. E.
700 1 $aSILVA, F. R. da
700 1 $aANDRADE, A. C.
773 $tJournal of Experimental Botany, Oxford$gv. 63, n. 11, p. 4191-4212, 2012.
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