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Registro Completo |
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Biblioteca(s): |
Biblioteca Rui Tendinha. |
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Data corrente: |
09/09/2020 |
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Data da última atualização: |
09/09/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
DUARTE, M. F.; PEREIRA-CARVALHO, R. de C.; REIS, L. de N. A. dos; ROJAS, M. J.; GILBERTSON, R.; COSTA, H.; BOITEUX, L. S.; FONSECA, M. E. N. |
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Afiliação: |
Macaria Ferreira Duarte, UnB; Rita de Cássia Pereira Carvalho, UnB; Luciane de Nazaré Almeida dos Reis, UnB; Maria J. Rojas, UC-DAVIS; Robert Gilbertson, UC-DAVIS; Helcio Costa, Incaper; Leonardo Silva Boiteux, EMBRAPA; Maria Esther N. Fonseca, EMBRAPA. |
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Título: |
Natural Infection of Tomatoes (Solanum lycopersicum) by Euphorbia yellow mosaic virus Isolates across Four Brazilian States. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Plant Disease, set. 2020. |
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Idioma: |
Inglês |
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Conteúdo: |
Severe yield losses induced by a complex of whitefly?transmitted Begomovirus species (family Geminiviridae) have been reported in tomatoes in Brazil (Reis et al. 2020). Nine isolates were obtained from tomato plants exhibiting begomovirus?like symptoms (viz. apical and interveinal chlorosis, yellow spots, and stunting) during independent field surveys: one isolate in Sumaré, São Paulo?SP State (isolate SP?066) in 2001, two in Serra Negra, Minas Gerais?MG (MG?012 and MG?016) in 2002, five in Caxias do Sul, Rio Grande do Sul?RS (RS?039, RS?045, RS?046, RS?047 and RS?058) in 2011 and one in Domingos Martins, Espírito Santo?ES (ES?148) in 2016. Disease incidence across all sampled fields ranged from 30% (in Domingos Martins?ES) to 90% in Sumaré?SP. Total DNA extraction was done by a modified CTAB method (Boiteux et al., 1999). Begomovirus infection was confirmed in all isolates by selective amplification of viral DNA?A segments using the primer pairs ?PAL1v1978 / PAR1c496? (Rojas et al., 1993) and ?BegomoAFor1? / ?BegomoARev1? (Ha et al., 2006), which produce two large and non?overlapping segments (≈1120 bp and ≈1205 bp, respectively). These PCR amplicons were initially characterized via direct Sanger dideoxy sequencing at CNPH. BLASTn analysis of the partial DNA?A genomes of these nine isolates indicated identity levels of 95?97% to three euphorbia yellow mosaic virus (EuYMV) reference isolates (= KY559532, JF756674, and KY559583) found infecting the weed Euphorbia heterophylla L. The entire DNA?A (2,609 nts = MN746971) and DNA?B (2,579 nts = MN746970) components of the MG?016 isolate were obtained via high?performance sequencing using Illumina HiSeq 2500 system (Macrogen Inc., South Korea). Sequences were assembled with the CLC Genomics Workbench program 10. Contigs were validated by BLASTx and BLASTn and compared to the ssDNA virus database at NCBI (www.ncbi.nlm.nih.gov). The fully?characterized MG?016 isolate displayed identity levels ranging from 97 to 99% to the EuYMV reference isolates as well as similar genomic features such as the conserved TATA box, nonanucleotide, and iterons (that were in agreement with a cognate nature of the DNA?A and DNA?B components). A partial sequence of the DNA?B genome was also obtained for the MG?012 isolate (MT7831942). The isolates MG?012 and MG?016 were found in mixed infections with tomato severe rugose virus (ToSRV) and tomato golden vein virus (TGVV), respectively. In addition, the complete DNA?A genomes of ES?148 (MN746972) and SP?066 (MN782438) were also obtained via a combination of primer walking and Sanger dideoxy sequencing, displaying 96?98% identity to EuYMV isolates. To our knowledge, this is the first report of multiple and independent events of natural infection of tomatoes by EuYMV isolates. Our results confirm the natural host status of tomatoes to EuYMV isolates as indicated in previous infectivity assays using biolistic inoculation (Barreto et al., 2013). The weed E. heterophylla is widely disseminated and very often present within tomato fields due to its higher levels of tolerance to the major herbicide (metribuzin) employed in this crop. Therefore, this weed may act as a persistent reservoir of tomato?infecting EuYMV isolates, which may allow the selection of viral populations potentially more adapted to this vegetable crop. MenosSevere yield losses induced by a complex of whitefly?transmitted Begomovirus species (family Geminiviridae) have been reported in tomatoes in Brazil (Reis et al. 2020). Nine isolates were obtained from tomato plants exhibiting begomovirus?like symptoms (viz. apical and interveinal chlorosis, yellow spots, and stunting) during independent field surveys: one isolate in Sumaré, São Paulo?SP State (isolate SP?066) in 2001, two in Serra Negra, Minas Gerais?MG (MG?012 and MG?016) in 2002, five in Caxias do Sul, Rio Grande do Sul?RS (RS?039, RS?045, RS?046, RS?047 and RS?058) in 2011 and one in Domingos Martins, Espírito Santo?ES (ES?148) in 2016. Disease incidence across all sampled fields ranged from 30% (in Domingos Martins?ES) to 90% in Sumaré?SP. Total DNA extraction was done by a modified CTAB method (Boiteux et al., 1999). Begomovirus infection was confirmed in all isolates by selective amplification of viral DNA?A segments using the primer pairs ?PAL1v1978 / PAR1c496? (Rojas et al., 1993) and ?BegomoAFor1? / ?BegomoARev1? (Ha et al., 2006), which produce two large and non?overlapping segments (≈1120 bp and ≈1205 bp, respectively). These PCR amplicons were initially characterized via direct Sanger dideoxy sequencing at CNPH. BLASTn analysis of the partial DNA?A genomes of these nine isolates indicated identity levels of 95?97% to three euphorbia yellow mosaic virus (EuYMV) reference isolates (= KY559532, JF756674, and KY559583) found infecting the weed Euphorbia ... Mostrar Tudo |
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Thesagro: |
Doença de Planta; Erva Daninha; Mosaico; Tomate. |
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Thesaurus NAL: |
Begomovirus. |
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Categoria do assunto: |
H Saúde e Patologia |
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URL: |
https://biblioteca.incaper.es.gov.br/digital/bitstream/123456789/4073/1/natural-infection-tomatoes-costa.pdf
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Marc: |
LEADER 04125naa a2200265 a 4500
001 1022277
005 2020-09-09
008 2020 bl uuuu u00u1 u #d
100 1 $aDUARTE, M. F.
245 $aNatural Infection of Tomatoes (Solanum lycopersicum) by Euphorbia yellow mosaic virus Isolates across Four Brazilian States.$h[electronic resource]
260 $c2020
520 $aSevere yield losses induced by a complex of whitefly?transmitted Begomovirus species (family Geminiviridae) have been reported in tomatoes in Brazil (Reis et al. 2020). Nine isolates were obtained from tomato plants exhibiting begomovirus?like symptoms (viz. apical and interveinal chlorosis, yellow spots, and stunting) during independent field surveys: one isolate in Sumaré, São Paulo?SP State (isolate SP?066) in 2001, two in Serra Negra, Minas Gerais?MG (MG?012 and MG?016) in 2002, five in Caxias do Sul, Rio Grande do Sul?RS (RS?039, RS?045, RS?046, RS?047 and RS?058) in 2011 and one in Domingos Martins, Espírito Santo?ES (ES?148) in 2016. Disease incidence across all sampled fields ranged from 30% (in Domingos Martins?ES) to 90% in Sumaré?SP. Total DNA extraction was done by a modified CTAB method (Boiteux et al., 1999). Begomovirus infection was confirmed in all isolates by selective amplification of viral DNA?A segments using the primer pairs ?PAL1v1978 / PAR1c496? (Rojas et al., 1993) and ?BegomoAFor1? / ?BegomoARev1? (Ha et al., 2006), which produce two large and non?overlapping segments (≈1120 bp and ≈1205 bp, respectively). These PCR amplicons were initially characterized via direct Sanger dideoxy sequencing at CNPH. BLASTn analysis of the partial DNA?A genomes of these nine isolates indicated identity levels of 95?97% to three euphorbia yellow mosaic virus (EuYMV) reference isolates (= KY559532, JF756674, and KY559583) found infecting the weed Euphorbia heterophylla L. The entire DNA?A (2,609 nts = MN746971) and DNA?B (2,579 nts = MN746970) components of the MG?016 isolate were obtained via high?performance sequencing using Illumina HiSeq 2500 system (Macrogen Inc., South Korea). Sequences were assembled with the CLC Genomics Workbench program 10. Contigs were validated by BLASTx and BLASTn and compared to the ssDNA virus database at NCBI (www.ncbi.nlm.nih.gov). The fully?characterized MG?016 isolate displayed identity levels ranging from 97 to 99% to the EuYMV reference isolates as well as similar genomic features such as the conserved TATA box, nonanucleotide, and iterons (that were in agreement with a cognate nature of the DNA?A and DNA?B components). A partial sequence of the DNA?B genome was also obtained for the MG?012 isolate (MT7831942). The isolates MG?012 and MG?016 were found in mixed infections with tomato severe rugose virus (ToSRV) and tomato golden vein virus (TGVV), respectively. In addition, the complete DNA?A genomes of ES?148 (MN746972) and SP?066 (MN782438) were also obtained via a combination of primer walking and Sanger dideoxy sequencing, displaying 96?98% identity to EuYMV isolates. To our knowledge, this is the first report of multiple and independent events of natural infection of tomatoes by EuYMV isolates. Our results confirm the natural host status of tomatoes to EuYMV isolates as indicated in previous infectivity assays using biolistic inoculation (Barreto et al., 2013). The weed E. heterophylla is widely disseminated and very often present within tomato fields due to its higher levels of tolerance to the major herbicide (metribuzin) employed in this crop. Therefore, this weed may act as a persistent reservoir of tomato?infecting EuYMV isolates, which may allow the selection of viral populations potentially more adapted to this vegetable crop.
650 $aBegomovirus
650 $aDoença de Planta
650 $aErva Daninha
650 $aMosaico
650 $aTomate
700 1 $aPEREIRA-CARVALHO, R. de C.
700 1 $aREIS, L. de N. A. dos
700 1 $aROJAS, M. J.
700 1 $aGILBERTSON, R.
700 1 $aCOSTA, H.
700 1 $aBOITEUX, L. S.
700 1 $aFONSECA, M. E. N.
773 $tPlant Disease, set. 2020.
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Registro original: |
Biblioteca Rui Tendinha (BRT) |
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| Registros recuperados : 175 | |
| 22. |  | MAXIMINO, S. C.; DUTRA, J. A. P.; RODRIGUES, R. P.; GONÇALVES, R. de C. R.; MORAIS, P. A. B.; VENTURA, J. A.; SCHUENCK, R. P.; LACERDA JÚNIOR, V.; KITAGAWA, R. R.; BORGES, W. de S. Synthesis of eugenol derivatives and evaluation of their antifungal activity against Fusarium solani f. sp. piperis. Current Pharmaceutical Design, v. 26, p. 1-11, 2020.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Biblioteca Rui Tendinha. |
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| 23. | | VENTURA, J. A.; LIMA, I. de M.; MARTINS, M. V. V.; CULIK, M. P.; COSTA, H. Impact and management of diseases in the propagation of fruit plants. Revista Brasileira de Fruticultura, Jaboticabal, v. 41, n. 4, e-647, 2019.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: - - - |
| Biblioteca(s): Biblioteca Rui Tendinha. |
|  |
| 24. | | CORREIA, A. de O.; SILVA, M. V. da.; CABANEZ, P. A.; ALVES, F. R.; COSTA, A. V.; QUEIROZ, V. T. de; XAVIER, A. da S.; COSTA, D. da C. O declínio da goiabeira : desafios e novas perspectivas para o manejo. In: SANTOS, C. A. dos (org.). Produção e manejo de culturas agrícolas de importância econômica [recurso eletrônico]. Maringá, PR, UNIEDUSUL, cap. 1, p. 1-17.| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Biblioteca Rui Tendinha. |
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| 26. | | NEVES, F. L.; NEVES, J. D. C.; POSSE, S. C. P.; PEREIRA, J. Ocorrência de Ustilago maydis em áreas de plantio de milho no município de Mucurici, Espírito Santo. In: JORNADA DE INTEGRAÇÃO DO IFES, 3., 2019. JORNADA DE INICIAÇÃO CIENTÍFICA, DESENVOLVIMENTO TECNOLÓGICO E INOVAÇÃO DO IFES, 14., 2019. SEMINÁRIO DE INICIAÇÃO CIENTÍFICA E TECNOLÓGICA (SICT) DO INCAPER, 3., 2018 e 2019. Alegre, ES : IFES; Incaper, 2019. Resumo.| Tipo: Publicação em Anais de Congresso |
| Biblioteca(s): Biblioteca Rui Tendinha. |
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| 29. | | KUHLCAMP, K. T.; MACÊDO, I.; BARRO, F. L. de S.; MOREIRA, S. O. Métodos de avaliação e parâmetros genéticos de pinta-preta em famílias de meio-irmãos de mamoeiro. In: SIMPÓSIO DO PAPAYA BRASILEIRO, 7. , Vitória, ES. Produção e sustentabilidade hídrica. Anais... [recurso eletrônico] / organizador, David dos Santos Martins. Vitória, ES : Incaper, 2018.| Tipo: Publicação em Anais de Congresso |
| Biblioteca(s): Biblioteca Rui Tendinha. |
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| 30. | | LOPES, U. P.; ALFENAS, R. A.; ZAMBOLIM, L.; CROUS, P. W.; COSTA, H.; PEREIRA, O. L. A new species of Calonectria causing rot on ripe strawberry fruit in Brazil. Australasian Plant Pathology, v. 47, n. 1, p. 1-11, 2018.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Biblioteca Rui Tendinha. |
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| 31. | | MELO, L. C.; PEREIRA, H. S.; FARIA, L. C. de; SOUZA, T. L. P. O. de; WENDLAND, A.; CABRERA DIAZ, J. L.; CARVALHO, H. W. L. de; MELO, C. L. P. de; COSTA, A. F. da; MAGALDI, M. C. de S.; COSTA, J. G. C. da; AGUIAR, M. S. de; ABREU, A. de F. B.; PEREIRA FILHO, I. A.; POSSE, S. C. P.; MARTINS, M.; ALBRECHT, J. C.; SOUZA FILHO, B. F. de; CARNEIRO, G. E. de S.; GUIMARAES, C. M.; BRAZ, A. J. B. P.; MARANGON, M. A.; TRINDADE, N. L. S. R.; SOUZA, N. P. de; FARIA, J. C. de; DEL PELOSO, M. J. BRS FC402: cultivar de feijão-comum carioca com alta produtividade, resistência à antracnose e murcha-de-fusário. Santo Antônio de Goiás: Embrapa Arroz e Feijão, 2017. 4 p. (Embrapa Arroz e Feijão. Comunicado técnico, 237).| Biblioteca(s): Biblioteca Rui Tendinha. |
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| 32. | | COSTA, A. F. da.; TARDIN, F. D.; COSTA, H.; ROSSI, D. A.; CAMPOS, W. F.; OLIVEIRA, A. R. de.; VIANA, A. P.; RODRIGUES, R.; PEREIRA, M. G. Comportamento de cultivares de videira em relação ao míldio. Revista Científica Intelletto, Venda Nova do Imigrante, ES, v.2, n.2, p.28-34, 2017. 525-9075| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 5 |
| Biblioteca(s): Biblioteca Rui Tendinha. |
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| 33. | | COSMI, F. C.; ALVES, K. dos S.; MORAES, W. B.; VENTURA, J. A.; MORAES, S. de P. C. B.; MORAES, W. B.; JESUS JÚNIOR, W. C. de Análise epidemiológica da evolução temporal da meleira do mamoeiro. SUMMA PHYTOPATHOLOGICA, v. 43, p. 303-309, 2017.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Biblioteca Rui Tendinha. |
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| 34. | | ROSA, R.; MARTINS, D. dos S.; GOMES, C. F.; ALVES, W. S. B.; SOUZA, C. A. S.; GOUVEA, R. R.; FORNAZIER, M. J.; QUEIROZ, R. B.; VENTURA, J. A. Infestação de Stenoma decora Zeller em cacaueiros no Norte do Estado do Espírito Santo. In: SEMINÁRIO DE INICIAÇÃO CIENTÍFICA E TECNOLÓGICA (SICT) DO INCAPER, 2., 2017. JORNADA DE INICIAÇÃO CIENTÍFICA, DESENVOLVIMENTO TECNOLÓGICO E INOVAÇÃO DO IFES, 12., 2017. Colatina, ES : IFES; Incaper, 2017. Resumo.| Tipo: Publicação em Anais de Congresso |
| Biblioteca(s): Biblioteca Rui Tendinha. |
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| 35. | | KROHLING, C. A.; ALIXANDRE, F. T.; ZUCCON, E.; COTA, M. C.; FERNANDES, L.H.M.; VENTURIM, C. H. P.; SARAIVA, U. Resultados agronômicos e fisiológicos do cafeeiro com o uso de fungicidas na pré e pós-florada. In: CONGRESSO BRASILEIRO DE PESQUISAS CAFEEIRAS, 43., 2017, Poços de Caldas. Novas tecnologias para um bom café produzir. Brasília, DF: Embrapa Café, 2017.| Tipo: Publicação em Anais de Congresso |
| Biblioteca(s): Biblioteca Rui Tendinha. |
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| 37. | | UENO, B.; COSTA, H. Doenças causadas por fungos e bactérias. In: ANTUNES, L. E. C.; REISSER JUNIOR, C.; SCHWENGBER, J. E. (Ed.). Morangueiro. Pelotas: Embrapa Clima Temperado: Brasília, DF: Embrapa, 2016. 589 p. p. 413-480| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Biblioteca Rui Tendinha. |
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| 39. | | MATIELLO, J. B.; ALMEIDA, I. B.; FERREIRA, M. B. da S.; CARVALHO, C. H. S. de.; KROHLING, C. A. Comportamento de progenies de cafeeiros com resistência à ferrugem, selecionadas de ensaios em vários campos experimentais do Procafé. In: CONGRESSO BRASILEIRO DE PESQUISAS CAFEEIRAS, 41., 2015, Poços de Caldas. Com mais tecnologia, o melhor café se aprecia: trabalhos apresentados. Varginha: Fundação Procafé, p. 364, 2015.| Tipo: Publicação em Anais de Congresso |
| Biblioteca(s): Biblioteca Rui Tendinha. |
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| 40. | | KROHLING, C. A.; MATIELLO, J. B.; ALMEIDA, S. R; PEREIRA, V. R.; KROHLING, C. C. K. Avaliação do ataque da mancha de Phoma em variedades de café arábica. In: CONGRESSO BRASILEIRO DE PESQUISAS CAFEEIRAS, 41., 2015, Poços de Caldas. Com mais tecnologia, mas café se aprecia: trabalhos apresentados. Poços de Caldas: Fundação Procafé, 2015. 2 p.| Tipo: Publicação em Anais de Congresso |
| Biblioteca(s): Biblioteca Rui Tendinha. |
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| Registros recuperados : 175 | |
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