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Biblioteca(s): |
Biblioteca Rui Tendinha. |
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Data corrente: |
13/01/2015 |
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Data da última atualização: |
13/01/2015 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
MARRACCINI, P.; VINECKY, F.; ALVES, G. S. C.; RAMOS, H. J. O.; ELBELT, S.; VIEIRA, N. G.; CARNEIRO, F. A.; SUJII, P. S.; ALEKCEVETCH, J. C.; SILVA, V. A.; DaMATTA, F. M.; FERRÃO, M. A. G.; LEROY, T.; POT, D.; VIEIRA, L. G. E.; SILVA, F. R. da; ANDRADE, A. C. |
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Afiliação: |
Maria Amélia Gava Ferrão, Incaper/Embrapa Café. |
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Título: |
Differentially expressed genes and proteins upon drought acclimation in tolerant and sensitive genotypes of Coffea canephora. |
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Ano de publicação: |
2012 |
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Fonte/Imprenta: |
Journal of Experimental Botany, Oxford v. 63, n. 11, p. 4191-4212, 2012. |
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Idioma: |
Inglês |
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Conteúdo: |
The aim of this study was to investigate the molecular mechanisms underlying drought acclimation in coffee plants by the identification of candidate genes (CGs) using different approaches. The first approach used the data generated during the Brazilian Coffee expressed sequence tag (EST) project to select 13 CGs by an in silico analysis (electronic northern). The second approach was based on screening macroarrays spotted with plasmid DNA (coffee ESTs) with separate hybridizations using leaf cDNA probes from drought-tolerant and susceptible clones of Coffea canephora var. Conilon, grown under different water regimes. This allowed the isolation of seven additional CGs. The third approach used two-dimensional gel electrophoresis to identify proteins displaying differential accumulation in leaves of drought-tolerant and susceptible clones of C. canephora. Six of them were characterized by MALDI-TOF-MS/MS (matrix-assisted laser desorption-time of flight-tandem mass spectrometry) and the corresponding proteins were identified. Finally, additional CGs were selected from the literature, and quantitative real-time polymerase chain reaction (qPCR) was performed to analyse the expression of all identified CGs. Altogether, >40 genes presenting differential gene expression during drought acclimation were identified, some of them showing different expression profiles between drought-tolerant and susceptible clones. Based on the obtained results, it can be concluded that factors involved a complex network of responses probably involving the abscisic signalling pathway and nitric oxide are major molecular determinants that might explain the better efficiency in controlling stomata closure and transpiration displayed by drought-tolerant clones of C. canephora. MenosThe aim of this study was to investigate the molecular mechanisms underlying drought acclimation in coffee plants by the identification of candidate genes (CGs) using different approaches. The first approach used the data generated during the Brazilian Coffee expressed sequence tag (EST) project to select 13 CGs by an in silico analysis (electronic northern). The second approach was based on screening macroarrays spotted with plasmid DNA (coffee ESTs) with separate hybridizations using leaf cDNA probes from drought-tolerant and susceptible clones of Coffea canephora var. Conilon, grown under different water regimes. This allowed the isolation of seven additional CGs. The third approach used two-dimensional gel electrophoresis to identify proteins displaying differential accumulation in leaves of drought-tolerant and susceptible clones of C. canephora. Six of them were characterized by MALDI-TOF-MS/MS (matrix-assisted laser desorption-time of flight-tandem mass spectrometry) and the corresponding proteins were identified. Finally, additional CGs were selected from the literature, and quantitative real-time polymerase chain reaction (qPCR) was performed to analyse the expression of all identified CGs. Altogether, >40 genes presenting differential gene expression during drought acclimation were identified, some of them showing different expression profiles between drought-tolerant and susceptible clones. Based on the obtained results, it can be concluded that factors involved a... Mostrar Tudo |
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Palavras-Chave: |
Candidate gene; Coffea canephora; Differential expression; Drought acclimation; Genética; Proteomic; Proteômica; Real time PCR. |
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Thesagro: |
Café; Coffea canephora; Genetics. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 03034naa a2200445 a 4500
001 1004907
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008 2012 bl uuuu u00u1 u #d
100 1 $aMARRACCINI, P.
245 $aDifferentially expressed genes and proteins upon drought acclimation in tolerant and sensitive genotypes of Coffea canephora.$h[electronic resource]
260 $c2012
520 $aThe aim of this study was to investigate the molecular mechanisms underlying drought acclimation in coffee plants by the identification of candidate genes (CGs) using different approaches. The first approach used the data generated during the Brazilian Coffee expressed sequence tag (EST) project to select 13 CGs by an in silico analysis (electronic northern). The second approach was based on screening macroarrays spotted with plasmid DNA (coffee ESTs) with separate hybridizations using leaf cDNA probes from drought-tolerant and susceptible clones of Coffea canephora var. Conilon, grown under different water regimes. This allowed the isolation of seven additional CGs. The third approach used two-dimensional gel electrophoresis to identify proteins displaying differential accumulation in leaves of drought-tolerant and susceptible clones of C. canephora. Six of them were characterized by MALDI-TOF-MS/MS (matrix-assisted laser desorption-time of flight-tandem mass spectrometry) and the corresponding proteins were identified. Finally, additional CGs were selected from the literature, and quantitative real-time polymerase chain reaction (qPCR) was performed to analyse the expression of all identified CGs. Altogether, >40 genes presenting differential gene expression during drought acclimation were identified, some of them showing different expression profiles between drought-tolerant and susceptible clones. Based on the obtained results, it can be concluded that factors involved a complex network of responses probably involving the abscisic signalling pathway and nitric oxide are major molecular determinants that might explain the better efficiency in controlling stomata closure and transpiration displayed by drought-tolerant clones of C. canephora.
650 $aCafé
650 $aCoffea canephora
650 $aGenetics
653 $aCandidate gene
653 $aCoffea canephora
653 $aDifferential expression
653 $aDrought acclimation
653 $aGenética
653 $aProteomic
653 $aProteômica
653 $aReal time PCR
700 1 $aVINECKY, F.
700 1 $aALVES, G. S. C.
700 1 $aRAMOS, H. J. O.
700 1 $aELBELT, S.
700 1 $aVIEIRA, N. G.
700 1 $aCARNEIRO, F. A.
700 1 $aSUJII, P. S.
700 1 $aALEKCEVETCH, J. C.
700 1 $aSILVA, V. A.
700 1 $aDaMATTA, F. M.
700 1 $aFERRÃO, M. A. G.
700 1 $aLEROY, T.
700 1 $aPOT, D.
700 1 $aVIEIRA, L. G. E.
700 1 $aSILVA, F. R. da
700 1 $aANDRADE, A. C.
773 $tJournal of Experimental Botany, Oxford$gv. 63, n. 11, p. 4191-4212, 2012.
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| 5. |  | ALVES, F. de L.; BALBINO, J. M. de S.; SOUZA, M. A. de. Novos clones de laranja 'Bahia' citrus sinensis (L) osbeck, para o município de Guaçuí e região da Serra do Caparaó, Estado do Espírito Santo. In: SEMINÁRIO BRASILEIRO DE PRODUÇÃO INTEGRADA DE FRUTAS, 8., 2006, Vitória. Anais... Vitória: INCAPER, p. 137-138, 2006.| Tipo: Publicação em Anais de Congresso |
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| 6. | | SILVA, M. V. da.; OLIVEIRA, E. C.; LIRIO,V. C.; MACETTE, H. A.; ALIXANDRE, R. D.; SOUZA, M. A. de; SALES, R. A. de; RIBEIRO, L. V. Influência da altitude e do sombreamento na produção de mudas callas no Espírito Santo. In: CONGRESSO CAPIXABA DE PESQUISA AGROPECUÁRIA, 1., Vitória, ES. Anais 2021 : congresso capixaba de pesquisa agropecuária [recurso eletrônico]. Vitória, ES: Incaper, 2021. color. PDF ; 25,4 MB. E-book, no formato PDF. (Incaper, Documentos, 289). Pedro Luís Pereira Teixeira de Carvalho, Carlos Henrique Rodrigues de Oliveira, José Aires Ventura, Marcos Vinicius Winckler Caldeira e Romário Gava Ferrão, editores. p. 261-265| Tipo: Publicação em Anais de Congresso |
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| 7. | | ALVES, F. L.; BALBINO, J. M. de S.; SOBRINHO, A. P. da C.; SOUZA, M. A. de; COSTA, R. S. da.; RIVA-SOUZA, E. M.; GUARNIER, J. C. Novas cultivares de laranja para o município de Guaçuí, ES. Vitória, ES : Incaper, 2009. (Incaper. Documentos, 171).| Biblioteca(s): Biblioteca Rui Tendinha. |
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| 8. | | ALVES, F. L.; BALBINO, J. M. de S.; SOBRINHO, A. P. da C.; SOUZA, M. A. de; COSTA, R. S. da.; ESPOSTI, M. D. D.; BASSANI, E. B.; CLEMENTE, P. R.; SOARES, J. B.; TARDIN, F. D.; RIVA-SOUZA, E. M. Novas cultivares de laranja para o município de Guaçuí, ES. In: CONGRESSO BRASILEIRO DE FRUTICULTURA, 20.; ANNUAL MEETING OF THE INTERAMERICAN SOCIETY FOR TROPICAL HORTICULTURE, 54., 2008, Vitória. Frutas para todos: estratégias, tecnologias e visão sustentável: anais. Vitória: INCAPER: Sociedade Brasileira de Fruticultura, 2008. 8p.| Tipo: Publicação em Anais de Congresso |
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| 9. | | DE MUNER, L. H.; FERRÃO, R. G.; PREZOTTI, L. C.; SILVA, A. E. S. da.; COSTA, E. B. da.; LANI, J. A.; GUARÇONI, A. M.; FONSECA, A. F. A. da.; FERRÃO, M. A. G.; TÓTOLA, E. F.; BOEQUI, G. Z.; MELO, E. V.; COSTA, A. N. da.; GROBÉRIO, J. C.; SOUZA, M. A. de; BASSANI, L. A.; TÓFANO, G.; BOREL, R. M. A.; FERRÃO, L. M. V. Calcário Correto : programa de incentivo à utilização de calcário para a cultura do café na região sul do estado do Espírito Santo. Vitória-ES : INCAPER, 2013. il. (INCAPER. Documentos, 225).| Biblioteca(s): Biblioteca Rui Tendinha. |
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| 10. | | ALVES, F. L.; CAIADO, L. H. L.; ESPOSTI, M. D.; CAETANO, L. C. S.; GOMES, S. A.; SILVA, D. N. da.; COSTA, A. de F. S. da.; TÓFANO, G.; VIAL, J. G; SHALDERS, P.; BREGONCI, I. dos S.; PINHEIRO, R. E.; FRUTUOSO, N. das N.; SOUZA, M. A. de; DALMONECH, J. C. M.; HASSEM, A. de P.; LIMA, G. C. de.; RODRIGUES, O. O. de A.; MENOSSI, J. L.; MARQUES, P. C.; DARDENGO, A. C.; MARTINS, C. L.; TEBALDI, C. S. L.; CELIN, E.; BORGES JUNIOR, J. T. Polo de laranja da região Sul-Caparaó do estado do Espirito Santo. Vitoria, ES: Incaper, 2011. 15 cm. x 27 cm. (Incaper. Documentos, 199)| Biblioteca(s): Biblioteca Rui Tendinha; São Gabriel da Palha. |
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| 11. | | SILVA, A. E. S. da.; SILVA, A. T. da.; BALBINO, J. M. de S.; COSTA, A. de F. S. da.; SILVA, D. N. da.; VENTURA, J. A.; CELIN, E.; BRAVIN, L. M. M.; TORRES, A. F. F.; NORONHA, R. G. da. S.; COSTA, A. F. da.; TEIXEIRA, C. P.; FORNAZIER, M. J.; COSTA, H.; ABAURRE, M. E. O.; ANGELETTI, M. da P. A.; GUARÇONI M., A.; PREZOTTI, L. C.; BOREL, R. M. A.; WOELFFEL, A. T.; WOELTHER, C.; LANG, E. H. W.; HAND, A. L.; BRAUN, L.; CASAGRANDE, J. A.; CARMO FILHO, O. G. do.; MARTINUZZO, M. B.; MOURA, A. G. P.; SOUZA, M. A. de; MENOSSI, J. C.; RODRIGUES. A.; FRAGA, M. R.; SIMON, M. T.; SIMÕES, L. T. Morango mais saudável : morango monitorado e rastreado. Morango das monstanhas do Espírito Santo : qualidade com responsabilidade. Vitória, ES: Incaper, 2010. (Incaper. Documentos, 181).| Biblioteca(s): Biblioteca Rui Tendinha; São Gabriel da Palha; Sooretama. |
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| Registros recuperados : 11 | |
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