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Registro Completo |
Biblioteca(s): |
Biblioteca Rui Tendinha. |
Data corrente: |
15/09/2015 |
Data da última atualização: |
15/09/2015 |
Autoria: |
AHRENS, D. C. (Coord.). |
Título: |
Rede de propriedades familiares agroecológicas: uma abordagem sistêmica no centro-sul do Paraná. |
Ano de publicação: |
2006 |
Fonte/Imprenta: |
Londrina: Instituto Agronômico do Paraná, 2006. |
Páginas: |
79 p. |
Série: |
(IAPAR. Boletim técnico, 68). |
ISSN: |
0100-3054 |
Idioma: |
Português |
Conteúdo: |
Caracterização regional; Descrição do recurso natural - potencializadades e restrições; Histórico de ocupação econômica; Evolução dos sistemas de produção; Realidade atual da agricultura regional; Agroindústria familiar; Os sistemas de produção agroecológicos em transição; Tipificação; Gestão das propriedades da rede; Pressão sobre o recurso natural; Descrição dos indicadores; Avaliação do uso da mão-de-obra; Capital empregado nos sistemas; Renda bruta nos sistemas; Margem bruta nos sistemas; Outras rendas; Experiências locais de geração de renda; Contribuições iniciais da rede de propriedades familiares agroecológicas; Interação entre pesquisa, extensão, universidade e agricultores; A rede com suas primeiras avaliações qualitativas; A rede e as questões ambientais legais. |
Palavras-Chave: |
Agricultura ecológica; Agricultura familiar; Agroecologia; Agroindústria; agroindústria familiar; Brasil; Ecologia agrícola; Meio ambiente; Ocupação; Palavras-chave: Agricultura familiar; Paraná; Pequeno produtor; Produção agrícola; Produção agropecuária; Propriedade agroecológica; Propriedade familiar; Propriedade familiar agroecológica; questões ambientais legais; recurso natural; Região centro-sul; Renda; Sistema de produção; Sistemas de produção; sistemas de produção agroecológicos; Uso da terra. |
Categoria do assunto: |
-- |
Marc: |
LEADER 02184nam a2200445 a 4500 001 1008172 005 2015-09-15 008 2006 bl uuuu 00u1 u #d 022 $a0100-3054 100 1 $aAHRENS, D. C. (Coord.). 245 $aRede de propriedades familiares agroecológicas$buma abordagem sistêmica no centro-sul do Paraná. 260 $aLondrina: Instituto Agronômico do Paraná$c2006 300 $a79 p. 490 $a(IAPAR. Boletim técnico, 68). 520 $aCaracterização regional; Descrição do recurso natural - potencializadades e restrições; Histórico de ocupação econômica; Evolução dos sistemas de produção; Realidade atual da agricultura regional; Agroindústria familiar; Os sistemas de produção agroecológicos em transição; Tipificação; Gestão das propriedades da rede; Pressão sobre o recurso natural; Descrição dos indicadores; Avaliação do uso da mão-de-obra; Capital empregado nos sistemas; Renda bruta nos sistemas; Margem bruta nos sistemas; Outras rendas; Experiências locais de geração de renda; Contribuições iniciais da rede de propriedades familiares agroecológicas; Interação entre pesquisa, extensão, universidade e agricultores; A rede com suas primeiras avaliações qualitativas; A rede e as questões ambientais legais. 653 $aAgricultura ecológica 653 $aAgricultura familiar 653 $aAgroecologia 653 $aAgroindústria 653 $aagroindústria familiar 653 $aBrasil 653 $aEcologia agrícola 653 $aMeio ambiente 653 $aOcupação 653 $aPalavras-chave: Agricultura familiar 653 $aParaná 653 $aPequeno produtor 653 $aProdução agrícola 653 $aProdução agropecuária 653 $aPropriedade agroecológica 653 $aPropriedade familiar 653 $aPropriedade familiar agroecológica 653 $aquestões ambientais legais 653 $arecurso natural 653 $aRegião centro-sul 653 $aRenda 653 $aSistema de produção 653 $aSistemas de produção 653 $asistemas de produção agroecológicos 653 $aUso da terra
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Biblioteca Rui Tendinha (BRT) |
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Registro Completo |
Biblioteca(s): |
Biblioteca Rui Tendinha. |
Data corrente: |
09/09/2020 |
Data da última atualização: |
09/09/2020 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 1 |
Autoria: |
DUARTE, M. F.; PEREIRA-CARVALHO, R. de C.; REIS, L. de N. A. dos; ROJAS, M. J.; GILBERTSON, R.; COSTA, H.; BOITEUX, L. S.; FONSECA, M. E. N. |
Afiliação: |
Macaria Ferreira Duarte, UnB; Rita de Cássia Pereira Carvalho, UnB; Luciane de Nazaré Almeida dos Reis, UnB; Maria J. Rojas, UC-DAVIS; Robert Gilbertson, UC-DAVIS; Helcio Costa, Incaper; Leonardo Silva Boiteux, EMBRAPA; Maria Esther N. Fonseca, EMBRAPA. |
Título: |
Natural Infection of Tomatoes (Solanum lycopersicum) by Euphorbia yellow mosaic virus Isolates across Four Brazilian States. |
Ano de publicação: |
2020 |
Fonte/Imprenta: |
Plant Disease, set. 2020. |
Idioma: |
Inglês |
Conteúdo: |
Severe yield losses induced by a complex of whitefly?transmitted Begomovirus species (family Geminiviridae) have been reported in tomatoes in Brazil (Reis et al. 2020). Nine isolates were obtained from tomato plants exhibiting begomovirus?like symptoms (viz. apical and interveinal chlorosis, yellow spots, and stunting) during independent field surveys: one isolate in Sumaré, São Paulo?SP State (isolate SP?066) in 2001, two in Serra Negra, Minas Gerais?MG (MG?012 and MG?016) in 2002, five in Caxias do Sul, Rio Grande do Sul?RS (RS?039, RS?045, RS?046, RS?047 and RS?058) in 2011 and one in Domingos Martins, Espírito Santo?ES (ES?148) in 2016. Disease incidence across all sampled fields ranged from 30% (in Domingos Martins?ES) to 90% in Sumaré?SP. Total DNA extraction was done by a modified CTAB method (Boiteux et al., 1999). Begomovirus infection was confirmed in all isolates by selective amplification of viral DNA?A segments using the primer pairs ?PAL1v1978 / PAR1c496? (Rojas et al., 1993) and ?BegomoAFor1? / ?BegomoARev1? (Ha et al., 2006), which produce two large and non?overlapping segments (≈1120 bp and ≈1205 bp, respectively). These PCR amplicons were initially characterized via direct Sanger dideoxy sequencing at CNPH. BLASTn analysis of the partial DNA?A genomes of these nine isolates indicated identity levels of 95?97% to three euphorbia yellow mosaic virus (EuYMV) reference isolates (= KY559532, JF756674, and KY559583) found infecting the weed Euphorbia heterophylla L. The entire DNA?A (2,609 nts = MN746971) and DNA?B (2,579 nts = MN746970) components of the MG?016 isolate were obtained via high?performance sequencing using Illumina HiSeq 2500 system (Macrogen Inc., South Korea). Sequences were assembled with the CLC Genomics Workbench program 10. Contigs were validated by BLASTx and BLASTn and compared to the ssDNA virus database at NCBI (www.ncbi.nlm.nih.gov). The fully?characterized MG?016 isolate displayed identity levels ranging from 97 to 99% to the EuYMV reference isolates as well as similar genomic features such as the conserved TATA box, nonanucleotide, and iterons (that were in agreement with a cognate nature of the DNA?A and DNA?B components). A partial sequence of the DNA?B genome was also obtained for the MG?012 isolate (MT7831942). The isolates MG?012 and MG?016 were found in mixed infections with tomato severe rugose virus (ToSRV) and tomato golden vein virus (TGVV), respectively. In addition, the complete DNA?A genomes of ES?148 (MN746972) and SP?066 (MN782438) were also obtained via a combination of primer walking and Sanger dideoxy sequencing, displaying 96?98% identity to EuYMV isolates. To our knowledge, this is the first report of multiple and independent events of natural infection of tomatoes by EuYMV isolates. Our results confirm the natural host status of tomatoes to EuYMV isolates as indicated in previous infectivity assays using biolistic inoculation (Barreto et al., 2013). The weed E. heterophylla is widely disseminated and very often present within tomato fields due to its higher levels of tolerance to the major herbicide (metribuzin) employed in this crop. Therefore, this weed may act as a persistent reservoir of tomato?infecting EuYMV isolates, which may allow the selection of viral populations potentially more adapted to this vegetable crop. MenosSevere yield losses induced by a complex of whitefly?transmitted Begomovirus species (family Geminiviridae) have been reported in tomatoes in Brazil (Reis et al. 2020). Nine isolates were obtained from tomato plants exhibiting begomovirus?like symptoms (viz. apical and interveinal chlorosis, yellow spots, and stunting) during independent field surveys: one isolate in Sumaré, São Paulo?SP State (isolate SP?066) in 2001, two in Serra Negra, Minas Gerais?MG (MG?012 and MG?016) in 2002, five in Caxias do Sul, Rio Grande do Sul?RS (RS?039, RS?045, RS?046, RS?047 and RS?058) in 2011 and one in Domingos Martins, Espírito Santo?ES (ES?148) in 2016. Disease incidence across all sampled fields ranged from 30% (in Domingos Martins?ES) to 90% in Sumaré?SP. Total DNA extraction was done by a modified CTAB method (Boiteux et al., 1999). Begomovirus infection was confirmed in all isolates by selective amplification of viral DNA?A segments using the primer pairs ?PAL1v1978 / PAR1c496? (Rojas et al., 1993) and ?BegomoAFor1? / ?BegomoARev1? (Ha et al., 2006), which produce two large and non?overlapping segments (≈1120 bp and ≈1205 bp, respectively). These PCR amplicons were initially characterized via direct Sanger dideoxy sequencing at CNPH. BLASTn analysis of the partial DNA?A genomes of these nine isolates indicated identity levels of 95?97% to three euphorbia yellow mosaic virus (EuYMV) reference isolates (= KY559532, JF756674, and KY559583) found infecting the weed Euphorbia ... Mostrar Tudo |
Thesagro: |
Doença de Planta; Erva Daninha; Mosaico; Tomate. |
Thesaurus NAL: |
Begomovirus. |
Categoria do assunto: |
H Saúde e Patologia |
URL: |
https://biblioteca.incaper.es.gov.br/digital/bitstream/123456789/4073/1/natural-infection-tomatoes-costa.pdf
|
Marc: |
LEADER 04125naa a2200265 a 4500 001 1022277 005 2020-09-09 008 2020 bl uuuu u00u1 u #d 100 1 $aDUARTE, M. F. 245 $aNatural Infection of Tomatoes (Solanum lycopersicum) by Euphorbia yellow mosaic virus Isolates across Four Brazilian States.$h[electronic resource] 260 $c2020 520 $aSevere yield losses induced by a complex of whitefly?transmitted Begomovirus species (family Geminiviridae) have been reported in tomatoes in Brazil (Reis et al. 2020). Nine isolates were obtained from tomato plants exhibiting begomovirus?like symptoms (viz. apical and interveinal chlorosis, yellow spots, and stunting) during independent field surveys: one isolate in Sumaré, São Paulo?SP State (isolate SP?066) in 2001, two in Serra Negra, Minas Gerais?MG (MG?012 and MG?016) in 2002, five in Caxias do Sul, Rio Grande do Sul?RS (RS?039, RS?045, RS?046, RS?047 and RS?058) in 2011 and one in Domingos Martins, Espírito Santo?ES (ES?148) in 2016. Disease incidence across all sampled fields ranged from 30% (in Domingos Martins?ES) to 90% in Sumaré?SP. Total DNA extraction was done by a modified CTAB method (Boiteux et al., 1999). Begomovirus infection was confirmed in all isolates by selective amplification of viral DNA?A segments using the primer pairs ?PAL1v1978 / PAR1c496? (Rojas et al., 1993) and ?BegomoAFor1? / ?BegomoARev1? (Ha et al., 2006), which produce two large and non?overlapping segments (≈1120 bp and ≈1205 bp, respectively). These PCR amplicons were initially characterized via direct Sanger dideoxy sequencing at CNPH. BLASTn analysis of the partial DNA?A genomes of these nine isolates indicated identity levels of 95?97% to three euphorbia yellow mosaic virus (EuYMV) reference isolates (= KY559532, JF756674, and KY559583) found infecting the weed Euphorbia heterophylla L. The entire DNA?A (2,609 nts = MN746971) and DNA?B (2,579 nts = MN746970) components of the MG?016 isolate were obtained via high?performance sequencing using Illumina HiSeq 2500 system (Macrogen Inc., South Korea). Sequences were assembled with the CLC Genomics Workbench program 10. Contigs were validated by BLASTx and BLASTn and compared to the ssDNA virus database at NCBI (www.ncbi.nlm.nih.gov). The fully?characterized MG?016 isolate displayed identity levels ranging from 97 to 99% to the EuYMV reference isolates as well as similar genomic features such as the conserved TATA box, nonanucleotide, and iterons (that were in agreement with a cognate nature of the DNA?A and DNA?B components). A partial sequence of the DNA?B genome was also obtained for the MG?012 isolate (MT7831942). The isolates MG?012 and MG?016 were found in mixed infections with tomato severe rugose virus (ToSRV) and tomato golden vein virus (TGVV), respectively. In addition, the complete DNA?A genomes of ES?148 (MN746972) and SP?066 (MN782438) were also obtained via a combination of primer walking and Sanger dideoxy sequencing, displaying 96?98% identity to EuYMV isolates. To our knowledge, this is the first report of multiple and independent events of natural infection of tomatoes by EuYMV isolates. Our results confirm the natural host status of tomatoes to EuYMV isolates as indicated in previous infectivity assays using biolistic inoculation (Barreto et al., 2013). The weed E. heterophylla is widely disseminated and very often present within tomato fields due to its higher levels of tolerance to the major herbicide (metribuzin) employed in this crop. Therefore, this weed may act as a persistent reservoir of tomato?infecting EuYMV isolates, which may allow the selection of viral populations potentially more adapted to this vegetable crop. 650 $aBegomovirus 650 $aDoença de Planta 650 $aErva Daninha 650 $aMosaico 650 $aTomate 700 1 $aPEREIRA-CARVALHO, R. de C. 700 1 $aREIS, L. de N. A. dos 700 1 $aROJAS, M. J. 700 1 $aGILBERTSON, R. 700 1 $aCOSTA, H. 700 1 $aBOITEUX, L. S. 700 1 $aFONSECA, M. E. N. 773 $tPlant Disease, set. 2020.
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