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 | Acesso ao texto completo restrito à biblioteca da Biblioteca Rui Tendinha. Para informações adicionais entre em contato com biblioteca@incaper.es.gov.br. |
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Registro Completo |
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Biblioteca(s): |
Biblioteca Rui Tendinha. |
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Data corrente: |
13/01/2015 |
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Data da última atualização: |
13/01/2015 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
MARRACCINI, P.; VINECKY, F.; ALVES, G. S. C.; RAMOS, H. J. O.; ELBELT, S.; VIEIRA, N. G.; CARNEIRO, F. A.; SUJII, P. S.; ALEKCEVETCH, J. C.; SILVA, V. A.; DaMATTA, F. M.; FERRÃO, M. A. G.; LEROY, T.; POT, D.; VIEIRA, L. G. E.; SILVA, F. R. da; ANDRADE, A. C. |
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Afiliação: |
Maria Amélia Gava Ferrão, Incaper/Embrapa Café. |
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Título: |
Differentially expressed genes and proteins upon drought acclimation in tolerant and sensitive genotypes of Coffea canephora. |
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Ano de publicação: |
2012 |
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Fonte/Imprenta: |
Journal of Experimental Botany, Oxford v. 63, n. 11, p. 4191-4212, 2012. |
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Idioma: |
Inglês |
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Conteúdo: |
The aim of this study was to investigate the molecular mechanisms underlying drought acclimation in coffee plants by the identification of candidate genes (CGs) using different approaches. The first approach used the data generated during the Brazilian Coffee expressed sequence tag (EST) project to select 13 CGs by an in silico analysis (electronic northern). The second approach was based on screening macroarrays spotted with plasmid DNA (coffee ESTs) with separate hybridizations using leaf cDNA probes from drought-tolerant and susceptible clones of Coffea canephora var. Conilon, grown under different water regimes. This allowed the isolation of seven additional CGs. The third approach used two-dimensional gel electrophoresis to identify proteins displaying differential accumulation in leaves of drought-tolerant and susceptible clones of C. canephora. Six of them were characterized by MALDI-TOF-MS/MS (matrix-assisted laser desorption-time of flight-tandem mass spectrometry) and the corresponding proteins were identified. Finally, additional CGs were selected from the literature, and quantitative real-time polymerase chain reaction (qPCR) was performed to analyse the expression of all identified CGs. Altogether, >40 genes presenting differential gene expression during drought acclimation were identified, some of them showing different expression profiles between drought-tolerant and susceptible clones. Based on the obtained results, it can be concluded that factors involved a complex network of responses probably involving the abscisic signalling pathway and nitric oxide are major molecular determinants that might explain the better efficiency in controlling stomata closure and transpiration displayed by drought-tolerant clones of C. canephora. MenosThe aim of this study was to investigate the molecular mechanisms underlying drought acclimation in coffee plants by the identification of candidate genes (CGs) using different approaches. The first approach used the data generated during the Brazilian Coffee expressed sequence tag (EST) project to select 13 CGs by an in silico analysis (electronic northern). The second approach was based on screening macroarrays spotted with plasmid DNA (coffee ESTs) with separate hybridizations using leaf cDNA probes from drought-tolerant and susceptible clones of Coffea canephora var. Conilon, grown under different water regimes. This allowed the isolation of seven additional CGs. The third approach used two-dimensional gel electrophoresis to identify proteins displaying differential accumulation in leaves of drought-tolerant and susceptible clones of C. canephora. Six of them were characterized by MALDI-TOF-MS/MS (matrix-assisted laser desorption-time of flight-tandem mass spectrometry) and the corresponding proteins were identified. Finally, additional CGs were selected from the literature, and quantitative real-time polymerase chain reaction (qPCR) was performed to analyse the expression of all identified CGs. Altogether, >40 genes presenting differential gene expression during drought acclimation were identified, some of them showing different expression profiles between drought-tolerant and susceptible clones. Based on the obtained results, it can be concluded that factors involved a... Mostrar Tudo |
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Palavras-Chave: |
Candidate gene; Coffea canephora; Differential expression; Drought acclimation; Genética; Proteomic; Proteômica; Real time PCR. |
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Thesagro: |
Café; Coffea canephora; Genetics. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 03034naa a2200445 a 4500
001 1004907
005 2015-01-13
008 2012 bl uuuu u00u1 u #d
100 1 $aMARRACCINI, P.
245 $aDifferentially expressed genes and proteins upon drought acclimation in tolerant and sensitive genotypes of Coffea canephora.$h[electronic resource]
260 $c2012
520 $aThe aim of this study was to investigate the molecular mechanisms underlying drought acclimation in coffee plants by the identification of candidate genes (CGs) using different approaches. The first approach used the data generated during the Brazilian Coffee expressed sequence tag (EST) project to select 13 CGs by an in silico analysis (electronic northern). The second approach was based on screening macroarrays spotted with plasmid DNA (coffee ESTs) with separate hybridizations using leaf cDNA probes from drought-tolerant and susceptible clones of Coffea canephora var. Conilon, grown under different water regimes. This allowed the isolation of seven additional CGs. The third approach used two-dimensional gel electrophoresis to identify proteins displaying differential accumulation in leaves of drought-tolerant and susceptible clones of C. canephora. Six of them were characterized by MALDI-TOF-MS/MS (matrix-assisted laser desorption-time of flight-tandem mass spectrometry) and the corresponding proteins were identified. Finally, additional CGs were selected from the literature, and quantitative real-time polymerase chain reaction (qPCR) was performed to analyse the expression of all identified CGs. Altogether, >40 genes presenting differential gene expression during drought acclimation were identified, some of them showing different expression profiles between drought-tolerant and susceptible clones. Based on the obtained results, it can be concluded that factors involved a complex network of responses probably involving the abscisic signalling pathway and nitric oxide are major molecular determinants that might explain the better efficiency in controlling stomata closure and transpiration displayed by drought-tolerant clones of C. canephora.
650 $aCafé
650 $aCoffea canephora
650 $aGenetics
653 $aCandidate gene
653 $aCoffea canephora
653 $aDifferential expression
653 $aDrought acclimation
653 $aGenética
653 $aProteomic
653 $aProteômica
653 $aReal time PCR
700 1 $aVINECKY, F.
700 1 $aALVES, G. S. C.
700 1 $aRAMOS, H. J. O.
700 1 $aELBELT, S.
700 1 $aVIEIRA, N. G.
700 1 $aCARNEIRO, F. A.
700 1 $aSUJII, P. S.
700 1 $aALEKCEVETCH, J. C.
700 1 $aSILVA, V. A.
700 1 $aDaMATTA, F. M.
700 1 $aFERRÃO, M. A. G.
700 1 $aLEROY, T.
700 1 $aPOT, D.
700 1 $aVIEIRA, L. G. E.
700 1 $aSILVA, F. R. da
700 1 $aANDRADE, A. C.
773 $tJournal of Experimental Botany, Oxford$gv. 63, n. 11, p. 4191-4212, 2012.
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Registro original: |
Biblioteca Rui Tendinha (BRT) |
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| Acesso ao texto completo restrito à biblioteca da Biblioteca Rui Tendinha. Para informações adicionais entre em contato com biblioteca@incaper.es.gov.br. |
|
Registro Completo |
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Biblioteca(s): |
Biblioteca Rui Tendinha. |
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Data corrente: |
10/01/2017 |
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Data da última atualização: |
10/01/2017 |
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Tipo da produção científica: |
Capítulo em Livro Técnico-Científico |
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Autoria: |
AL-SADI, A. M.; QUEIROZ, R. B.; DONKERSLEY, P.; NASEHI, A.; ELLIOT, S. L. |
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Afiliação: |
A.M. Al-Sadi, Sultan Qaboos University; Renan Batista Queiroz, Incaper; Philip Donkersley, UFV; A. Nasehi, Sultan Qaboos University; Simon L. Elliot, UFV. |
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Título: |
Plant Protection : lime diseases and insect pests. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
In: Khan, M. Mumtaz, editor. The lime (botany, production and uses). Boston, MA : CABI, p. 149-166, 2017. |
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Idioma: |
Inglês |
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Conteúdo: |
Acid lime is affected by several diseases and pests, resulting in variable losses, which range from negligible effects on leaves, roots, stems or fruits to reduction in growth and yield or complete decline. The distribution of acid lime pests and diseases and their severity are affected by several factors, including conduciveness of the environmental conditions, aggressiveness of pathogen strains, type of insect pests, and resistance of limes and rootstocks. Phytoplasmainduced witches? broom disease of lime is a serious
disease, especially in the Middle East. Citrus canker, Huanglongbing disease and several fungaland viral diseases are common in different parts of the world. Leafhoppers, psyllids and leaf miners are among the most damaging pests
of limes... |
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Thesaurus NAL: |
Acid Lime Pests; Anthracnose; Bemisia tabaci; Citrus canker; Citrus tristeza virus; Gummosis and foot; Hemiptera; Huanglongbing; Lime; Melanose; Pests; Plant protection. |
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Categoria do assunto: |
H Saúde e Patologia |
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Marc: |
LEADER 01638naa a2200313 a 4500
001 1013976
005 2017-01-10
008 2017 bl uuuu u00u1 u #d
100 1 $aAL-SADI, A. M.
245 $aPlant Protection$blime diseases and insect pests.$h[electronic resource]
260 $c2017
520 $aAcid lime is affected by several diseases and pests, resulting in variable losses, which range from negligible effects on leaves, roots, stems or fruits to reduction in growth and yield or complete decline. The distribution of acid lime pests and diseases and their severity are affected by several factors, including conduciveness of the environmental conditions, aggressiveness of pathogen strains, type of insect pests, and resistance of limes and rootstocks. Phytoplasmainduced witches? broom disease of lime is a serious disease, especially in the Middle East. Citrus canker, Huanglongbing disease and several fungaland viral diseases are common in different parts of the world. Leafhoppers, psyllids and leaf miners are among the most damaging pests of limes...
650 $aAcid Lime Pests
650 $aAnthracnose
650 $aBemisia tabaci
650 $aCitrus canker
650 $aCitrus tristeza virus
650 $aGummosis and foot
650 $aHemiptera
650 $aHuanglongbing
650 $aLime
650 $aMelanose
650 $aPests
650 $aPlant protection
700 1 $aQUEIROZ, R. B.
700 1 $aDONKERSLEY, P.
700 1 $aNASEHI, A.
700 1 $aELLIOT, S. L.
773 $tIn: Khan, M. Mumtaz, editor. The lime (botany, production and uses). Boston, MA : CABI, p. 149-166, 2017.
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