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 | Acesso ao texto completo restrito à biblioteca da Biblioteca Rui Tendinha. Para informações adicionais entre em contato com biblioteca@incaper.es.gov.br. |
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Registro Completo |
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Biblioteca(s): |
Biblioteca Rui Tendinha. |
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Data corrente: |
06/07/2020 |
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Data da última atualização: |
06/07/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
MOURA, R. D.; CASTRO, L. A. M. de; CULIK, M. P.; FERNANDES, A. A. R.; FERNANDES, P. M. B.; VENTURA, J. A. |
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Afiliação: |
Raíssa Debacker Moura; Luiza Adami Monteiro de Castro; Mark Paul Culik, CNPq/Incaper; Antônio Alberto Ribeiro Fernandes; Patricia Machado Bueno Fernandes; Jose Aires Ventura, Incaper. |
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Título: |
Culture medium for improved production of conidia for identification and systematic studies of Fusarium pathogens. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Journal of Microbiological Methods, v. 173, June 2020. |
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DOI: |
10.1016/j.mimet.2020.105915 |
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Idioma: |
Inglês |
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Conteúdo: |
Fusarium guttiforme and Fusarium ananatum are the etiological agents of fusariosis and fruitlet core rot in pineapple, espectively, producing mycotoxins that are harmful to the health of consumers. These two fungi are morphologically similar and difficulty in obtaining macroconidia of the species limits their identification. Different types of media are available for the culture of these pathogens, but not all of them favor F. ananatum
and F. guttiforme macroconidia production. Therefore, the objective of this study was to develop a simple culture medium to improve rapid macro- and microconidia formation in both F. guttiforme and F. ananatum to facilitate taxonomic, pathogenicity and mycotoxin studies. In vitro analysis showed that basal medium with carboxymethyl cellulose (CMC) was better than other media tested with the highest macroconidia production at 7 days of incubation. The highest production of microconidia was with synthetic nutrient medium (SN) at 7 days. F. ananatum produced a relatively high number of microconidia with one septum in comparison to F. guttiforme when cultured in CMC, which suggests an additional character useful for Fusarium taxonomy. CMC medium may serve as an improved alternative to culture media currently used in Fusarium research and contribute to further knowledge of the taxonomy and mycotoxins of Fusarium species. |
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Palavras-Chave: |
Abacaxi. |
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Thesagro: |
Fusariose; Fusarium; Patogenicidade; Taxonomia. |
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Thesaurus NAL: |
Carboxymethyl cellulose; Sporulation; Taxonomy. |
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Categoria do assunto: |
H Saúde e Patologia |
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Marc: |
LEADER 02225naa a2200289 a 4500
001 1022238
005 2020-07-06
008 2020 bl uuuu u00u1 u #d
024 7 $a10.1016/j.mimet.2020.105915$2DOI
100 1 $aMOURA, R. D.
245 $aCulture medium for improved production of conidia for identification and systematic studies of Fusarium pathogens.$h[electronic resource]
260 $c2020
520 $aFusarium guttiforme and Fusarium ananatum are the etiological agents of fusariosis and fruitlet core rot in pineapple, espectively, producing mycotoxins that are harmful to the health of consumers. These two fungi are morphologically similar and difficulty in obtaining macroconidia of the species limits their identification. Different types of media are available for the culture of these pathogens, but not all of them favor F. ananatum and F. guttiforme macroconidia production. Therefore, the objective of this study was to develop a simple culture medium to improve rapid macro- and microconidia formation in both F. guttiforme and F. ananatum to facilitate taxonomic, pathogenicity and mycotoxin studies. In vitro analysis showed that basal medium with carboxymethyl cellulose (CMC) was better than other media tested with the highest macroconidia production at 7 days of incubation. The highest production of microconidia was with synthetic nutrient medium (SN) at 7 days. F. ananatum produced a relatively high number of microconidia with one septum in comparison to F. guttiforme when cultured in CMC, which suggests an additional character useful for Fusarium taxonomy. CMC medium may serve as an improved alternative to culture media currently used in Fusarium research and contribute to further knowledge of the taxonomy and mycotoxins of Fusarium species.
650 $aCarboxymethyl cellulose
650 $aSporulation
650 $aTaxonomy
650 $aFusariose
650 $aFusarium
650 $aPatogenicidade
650 $aTaxonomia
653 $aAbacaxi
700 1 $aCASTRO, L. A. M. de
700 1 $aCULIK, M. P.
700 1 $aFERNANDES, A. A. R.
700 1 $aFERNANDES, P. M. B.
700 1 $aVENTURA, J. A.
773 $tJournal of Microbiological Methods$gv. 173, June 2020.
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Registro original: |
Biblioteca Rui Tendinha (BRT) |
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| Acesso ao texto completo restrito à biblioteca da Biblioteca Rui Tendinha. Para informações adicionais entre em contato com biblioteca@incaper.es.gov.br. |
|
Registro Completo |
|
Biblioteca(s): |
Biblioteca Rui Tendinha. |
|
Data corrente: |
11/06/2019 |
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Data da última atualização: |
09/12/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
- - - |
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Autoria: |
GOMES, R. B. de A.; SOUZA, E. S. de; BARRAQUI, N. S. G.; TOSTA, C. L.; NUNES, A. P. F.; SCHUENCK, R. P.; RUAS, F. G.; VENTURA, J. A.; FILGUEIRAS, P. R.; KUSTER, R. M. |
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Afiliação: |
Rodrigo Borges de Araujo Gomes, IFES ARACRUZ; Erica Santana de Souza, UFES; Nataly Senna Gerhardt Barraqui, UFES; Cristina Luz Tosta, UFES; Ana Paula Ferreira Nunes, UFES; Ricardo Pinto Schuenck, UFES; Fabiana Gomes Ruas, Incaper; Jose Aires Ventura, Incaper; Paulo Roberto Filgueiras, UFES; Ricardo Machado Kuster, UFES. |
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Título: |
Residues from the Brazilian pepper tree (Schinus terebinthifolia Raddi) processing industry: Chemical profile and antimicrobial activity of extracts against hospital bacteria. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
Industrial Crops and Products |
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Idioma: |
Inglês |
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Conteúdo: |
Schinus terebinthifolia Raddi is a plant used in folk medicine in the treatment of various diseases and has several biological potentials. Its fruit is used as condiment and has high demand in the spice market. In the present study extracts of different polarities prepared from residues from the Brazilian pepper tree processing industry were characterized chemically by gas chromatography/mass spectrometry (GC?MS) and negative-ion mode electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry (ESI(-)FT-ICR MS). The antibacterial activity of the extracts was evaluated against multidrug-resistant strains of hospital origin (Staphylococcus aureus, Enterococcus faecium, Enterococcus faecalis, Pseudomonas aeruginosa and Acinetobacter baumannii) and standard strains (ATCC). The apolar fractions (dichloromethane and hexane) presented triterpenes as main components and the polar extracts (methanol and hydroethanolic extracts) were characterized by high contents of phenolic compounds, especially gallotannins, gallic acid and flavonoids. The methanolic fraction and the hydroethanolic extract of the residues were the most active mainly against S. aureus (MIC 0.60?0.90?mg/mL), E. faecium and E. faecalis (MIC 1.20?2.10?mg/mL). These results demonstrate the richness of bioactive compounds present in the residues and indicate a possible application of this material for the development of biotechnological products with potential against multidrug-resistant bacteria. |
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Palavras-Chave: |
Aroeira; Pimenta-rosa. |
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Thesaurus NAL: |
Antimicrobial activity; Brazilian pepper; Phenolic compounds; Residues; Schinus terebinthifolia. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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Marc: |
LEADER 02463naa a2200313 a 4500
001 1021392
005 2019-12-09
008 2019 bl uuuu u00u1 u #d
100 1 $aGOMES, R. B. de A.
245 $aResidues from the Brazilian pepper tree (Schinus terebinthifolia Raddi) processing industry$bChemical profile and antimicrobial activity of extracts against hospital bacteria.$h[electronic resource]
260 $c2019
520 $aSchinus terebinthifolia Raddi is a plant used in folk medicine in the treatment of various diseases and has several biological potentials. Its fruit is used as condiment and has high demand in the spice market. In the present study extracts of different polarities prepared from residues from the Brazilian pepper tree processing industry were characterized chemically by gas chromatography/mass spectrometry (GC?MS) and negative-ion mode electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry (ESI(-)FT-ICR MS). The antibacterial activity of the extracts was evaluated against multidrug-resistant strains of hospital origin (Staphylococcus aureus, Enterococcus faecium, Enterococcus faecalis, Pseudomonas aeruginosa and Acinetobacter baumannii) and standard strains (ATCC). The apolar fractions (dichloromethane and hexane) presented triterpenes as main components and the polar extracts (methanol and hydroethanolic extracts) were characterized by high contents of phenolic compounds, especially gallotannins, gallic acid and flavonoids. The methanolic fraction and the hydroethanolic extract of the residues were the most active mainly against S. aureus (MIC 0.60?0.90?mg/mL), E. faecium and E. faecalis (MIC 1.20?2.10?mg/mL). These results demonstrate the richness of bioactive compounds present in the residues and indicate a possible application of this material for the development of biotechnological products with potential against multidrug-resistant bacteria.
650 $aAntimicrobial activity
650 $aBrazilian pepper
650 $aPhenolic compounds
650 $aResidues
650 $aSchinus terebinthifolia
653 $aAroeira
653 $aPimenta-rosa
700 1 $aSOUZA, E. S. de
700 1 $aBARRAQUI, N. S. G.
700 1 $aTOSTA, C. L.
700 1 $aNUNES, A. P. F.
700 1 $aSCHUENCK, R. P.
700 1 $aRUAS, F. G.
700 1 $aVENTURA, J. A.
700 1 $aFILGUEIRAS, P. R.
700 1 $aKUSTER, R. M.
773 $tIndustrial Crops and Products
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