| |
|
|
 | Acesso ao texto completo restrito à biblioteca da Biblioteca Rui Tendinha. Para informações adicionais entre em contato com biblioteca@incaper.es.gov.br. |
|
Registro Completo |
|
Biblioteca(s): |
Biblioteca Rui Tendinha. |
|
Data corrente: |
13/01/2015 |
|
Data da última atualização: |
13/01/2015 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Autoria: |
MARRACCINI, P.; VINECKY, F.; ALVES, G. S. C.; RAMOS, H. J. O.; ELBELT, S.; VIEIRA, N. G.; CARNEIRO, F. A.; SUJII, P. S.; ALEKCEVETCH, J. C.; SILVA, V. A.; DaMATTA, F. M.; FERRÃO, M. A. G.; LEROY, T.; POT, D.; VIEIRA, L. G. E.; SILVA, F. R. da; ANDRADE, A. C. |
|
Afiliação: |
Maria Amélia Gava Ferrão, Incaper/Embrapa Café. |
|
Título: |
Differentially expressed genes and proteins upon drought acclimation in tolerant and sensitive genotypes of Coffea canephora. |
|
Ano de publicação: |
2012 |
|
Fonte/Imprenta: |
Journal of Experimental Botany, Oxford v. 63, n. 11, p. 4191-4212, 2012. |
|
Idioma: |
Inglês |
|
Conteúdo: |
The aim of this study was to investigate the molecular mechanisms underlying drought acclimation in coffee plants by the identification of candidate genes (CGs) using different approaches. The first approach used the data generated during the Brazilian Coffee expressed sequence tag (EST) project to select 13 CGs by an in silico analysis (electronic northern). The second approach was based on screening macroarrays spotted with plasmid DNA (coffee ESTs) with separate hybridizations using leaf cDNA probes from drought-tolerant and susceptible clones of Coffea canephora var. Conilon, grown under different water regimes. This allowed the isolation of seven additional CGs. The third approach used two-dimensional gel electrophoresis to identify proteins displaying differential accumulation in leaves of drought-tolerant and susceptible clones of C. canephora. Six of them were characterized by MALDI-TOF-MS/MS (matrix-assisted laser desorption-time of flight-tandem mass spectrometry) and the corresponding proteins were identified. Finally, additional CGs were selected from the literature, and quantitative real-time polymerase chain reaction (qPCR) was performed to analyse the expression of all identified CGs. Altogether, >40 genes presenting differential gene expression during drought acclimation were identified, some of them showing different expression profiles between drought-tolerant and susceptible clones. Based on the obtained results, it can be concluded that factors involved a complex network of responses probably involving the abscisic signalling pathway and nitric oxide are major molecular determinants that might explain the better efficiency in controlling stomata closure and transpiration displayed by drought-tolerant clones of C. canephora. MenosThe aim of this study was to investigate the molecular mechanisms underlying drought acclimation in coffee plants by the identification of candidate genes (CGs) using different approaches. The first approach used the data generated during the Brazilian Coffee expressed sequence tag (EST) project to select 13 CGs by an in silico analysis (electronic northern). The second approach was based on screening macroarrays spotted with plasmid DNA (coffee ESTs) with separate hybridizations using leaf cDNA probes from drought-tolerant and susceptible clones of Coffea canephora var. Conilon, grown under different water regimes. This allowed the isolation of seven additional CGs. The third approach used two-dimensional gel electrophoresis to identify proteins displaying differential accumulation in leaves of drought-tolerant and susceptible clones of C. canephora. Six of them were characterized by MALDI-TOF-MS/MS (matrix-assisted laser desorption-time of flight-tandem mass spectrometry) and the corresponding proteins were identified. Finally, additional CGs were selected from the literature, and quantitative real-time polymerase chain reaction (qPCR) was performed to analyse the expression of all identified CGs. Altogether, >40 genes presenting differential gene expression during drought acclimation were identified, some of them showing different expression profiles between drought-tolerant and susceptible clones. Based on the obtained results, it can be concluded that factors involved a... Mostrar Tudo |
|
Palavras-Chave: |
Candidate gene; Coffea canephora; Differential expression; Drought acclimation; Genética; Proteomic; Proteômica; Real time PCR. |
|
Thesagro: |
Café; Coffea canephora; Genetics. |
|
Categoria do assunto: |
-- |
|
Marc: |
LEADER 03034naa a2200445 a 4500
001 1004907
005 2015-01-13
008 2012 bl uuuu u00u1 u #d
100 1 $aMARRACCINI, P.
245 $aDifferentially expressed genes and proteins upon drought acclimation in tolerant and sensitive genotypes of Coffea canephora.$h[electronic resource]
260 $c2012
520 $aThe aim of this study was to investigate the molecular mechanisms underlying drought acclimation in coffee plants by the identification of candidate genes (CGs) using different approaches. The first approach used the data generated during the Brazilian Coffee expressed sequence tag (EST) project to select 13 CGs by an in silico analysis (electronic northern). The second approach was based on screening macroarrays spotted with plasmid DNA (coffee ESTs) with separate hybridizations using leaf cDNA probes from drought-tolerant and susceptible clones of Coffea canephora var. Conilon, grown under different water regimes. This allowed the isolation of seven additional CGs. The third approach used two-dimensional gel electrophoresis to identify proteins displaying differential accumulation in leaves of drought-tolerant and susceptible clones of C. canephora. Six of them were characterized by MALDI-TOF-MS/MS (matrix-assisted laser desorption-time of flight-tandem mass spectrometry) and the corresponding proteins were identified. Finally, additional CGs were selected from the literature, and quantitative real-time polymerase chain reaction (qPCR) was performed to analyse the expression of all identified CGs. Altogether, >40 genes presenting differential gene expression during drought acclimation were identified, some of them showing different expression profiles between drought-tolerant and susceptible clones. Based on the obtained results, it can be concluded that factors involved a complex network of responses probably involving the abscisic signalling pathway and nitric oxide are major molecular determinants that might explain the better efficiency in controlling stomata closure and transpiration displayed by drought-tolerant clones of C. canephora.
650 $aCafé
650 $aCoffea canephora
650 $aGenetics
653 $aCandidate gene
653 $aCoffea canephora
653 $aDifferential expression
653 $aDrought acclimation
653 $aGenética
653 $aProteomic
653 $aProteômica
653 $aReal time PCR
700 1 $aVINECKY, F.
700 1 $aALVES, G. S. C.
700 1 $aRAMOS, H. J. O.
700 1 $aELBELT, S.
700 1 $aVIEIRA, N. G.
700 1 $aCARNEIRO, F. A.
700 1 $aSUJII, P. S.
700 1 $aALEKCEVETCH, J. C.
700 1 $aSILVA, V. A.
700 1 $aDaMATTA, F. M.
700 1 $aFERRÃO, M. A. G.
700 1 $aLEROY, T.
700 1 $aPOT, D.
700 1 $aVIEIRA, L. G. E.
700 1 $aSILVA, F. R. da
700 1 $aANDRADE, A. C.
773 $tJournal of Experimental Botany, Oxford$gv. 63, n. 11, p. 4191-4212, 2012.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Biblioteca Rui Tendinha (BRT) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Voltar
|
|
|
| Acesso ao texto completo restrito à biblioteca da Biblioteca Rui Tendinha. Para informações adicionais entre em contato com biblioteca@incaper.es.gov.br. |
|
Registro Completo |
|
Biblioteca(s): |
Biblioteca Rui Tendinha. |
|
Data corrente: |
16/08/2021 |
|
Data da última atualização: |
16/08/2021 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Circulação/Nível: |
B - 2 |
|
Autoria: |
ARAUJO, M. R.; MARTINS, D. dos S.; FORNAZIER, M. J.; URAMOTO, K.; FERREIRA, P. S. F.; ZUCCHI, R. A.; GODOY, W. A. C. |
|
Afiliação: |
Mayara R. de Araujo, ESALQ; David dos Santos Martins, Incaper; Mauricio José Fornazier, Incaper; Keiko Uramoto, ESALQ; Paulo S. F. Ferreira, UFV; Roberto A. Zucchi, UFV; Wesley A.C. Godoy, ESALQ. |
|
Título: |
Long-term fruit fly monitoring and impact of the systems approach on richness and abundance. |
|
Ano de publicação: |
2021 |
|
Fonte/Imprenta: |
The Canadian Entomologist, p. 1-20, 2021. |
|
Idioma: |
Inglês |
|
Conteúdo: |
We investigated ecological patterns of richness and abundance of Anastrepha fruit flies, based on a long-term series with a dataset from 1998 to 2010, subdivided into four subseries describing pest management through the systems approach (integration of different measures, at least two of which act independently, with cumulative effects), and its impact on abundance and diversity of fruit flies. Richness and abundance were influenced by time and distance but to different extents. Spatio-temporal analysis taking into account the implementation of the systems approach revealed
clear effects of the pest management on fruit fly richness and abundance. However, abundance was affected by the systems approach three years before richness was. Abundance and richness also showed different relationships with time and distance between orchards and forest. The Gompertz
model, used to describe the relationship between area and species richness, was the function that showed the best fit to the data. The richness-partitioning analysis, which decomposes beta diversity, indicated different distributions of richness values and predictions for additive partitioning that were directly associated with the implementation of the systems approach. The spectral analysis projected different trends for peaks, indicating that the systems approach is able to delay the time for new population peaks of fruit flies. |
|
Palavras-Chave: |
Modelo Gompertz. |
|
Thesagro: |
Mamão; Mosca das Frutas. |
|
Categoria do assunto: |
-- |
|
Marc: |
LEADER 02067naa a2200229 a 4500
001 1023652
005 2021-08-16
008 2021 bl uuuu u00u1 u #d
100 1 $aARAUJO, M. R.
245 $aLong-term fruit fly monitoring and impact of the systems approach on richness and abundance.$h[electronic resource]
260 $c2021
520 $aWe investigated ecological patterns of richness and abundance of Anastrepha fruit flies, based on a long-term series with a dataset from 1998 to 2010, subdivided into four subseries describing pest management through the systems approach (integration of different measures, at least two of which act independently, with cumulative effects), and its impact on abundance and diversity of fruit flies. Richness and abundance were influenced by time and distance but to different extents. Spatio-temporal analysis taking into account the implementation of the systems approach revealed clear effects of the pest management on fruit fly richness and abundance. However, abundance was affected by the systems approach three years before richness was. Abundance and richness also showed different relationships with time and distance between orchards and forest. The Gompertz model, used to describe the relationship between area and species richness, was the function that showed the best fit to the data. The richness-partitioning analysis, which decomposes beta diversity, indicated different distributions of richness values and predictions for additive partitioning that were directly associated with the implementation of the systems approach. The spectral analysis projected different trends for peaks, indicating that the systems approach is able to delay the time for new population peaks of fruit flies.
650 $aMamão
650 $aMosca das Frutas
653 $aModelo Gompertz
700 1 $aMARTINS, D. dos S.
700 1 $aFORNAZIER, M. J.
700 1 $aURAMOTO, K.
700 1 $aFERREIRA, P. S. F.
700 1 $aZUCCHI, R. A.
700 1 $aGODOY, W. A. C.
773 $tThe Canadian Entomologist, p. 1-20, 2021.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Biblioteca Rui Tendinha (BRT) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
| Expressão de busca inválida. Verifique!!! |
|
|
