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 | Acesso ao texto completo restrito à biblioteca da Biblioteca Rui Tendinha. Para informações adicionais entre em contato com biblioteca@incaper.es.gov.br. |
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Registro Completo |
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Biblioteca(s): |
Biblioteca Rui Tendinha. |
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Data corrente: |
13/01/2015 |
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Data da última atualização: |
13/01/2015 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
MARRACCINI, P.; VINECKY, F.; ALVES, G. S. C.; RAMOS, H. J. O.; ELBELT, S.; VIEIRA, N. G.; CARNEIRO, F. A.; SUJII, P. S.; ALEKCEVETCH, J. C.; SILVA, V. A.; DaMATTA, F. M.; FERRÃO, M. A. G.; LEROY, T.; POT, D.; VIEIRA, L. G. E.; SILVA, F. R. da; ANDRADE, A. C. |
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Afiliação: |
Maria Amélia Gava Ferrão, Incaper/Embrapa Café. |
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Título: |
Differentially expressed genes and proteins upon drought acclimation in tolerant and sensitive genotypes of Coffea canephora. |
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Ano de publicação: |
2012 |
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Fonte/Imprenta: |
Journal of Experimental Botany, Oxford v. 63, n. 11, p. 4191-4212, 2012. |
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Idioma: |
Inglês |
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Conteúdo: |
The aim of this study was to investigate the molecular mechanisms underlying drought acclimation in coffee plants by the identification of candidate genes (CGs) using different approaches. The first approach used the data generated during the Brazilian Coffee expressed sequence tag (EST) project to select 13 CGs by an in silico analysis (electronic northern). The second approach was based on screening macroarrays spotted with plasmid DNA (coffee ESTs) with separate hybridizations using leaf cDNA probes from drought-tolerant and susceptible clones of Coffea canephora var. Conilon, grown under different water regimes. This allowed the isolation of seven additional CGs. The third approach used two-dimensional gel electrophoresis to identify proteins displaying differential accumulation in leaves of drought-tolerant and susceptible clones of C. canephora. Six of them were characterized by MALDI-TOF-MS/MS (matrix-assisted laser desorption-time of flight-tandem mass spectrometry) and the corresponding proteins were identified. Finally, additional CGs were selected from the literature, and quantitative real-time polymerase chain reaction (qPCR) was performed to analyse the expression of all identified CGs. Altogether, >40 genes presenting differential gene expression during drought acclimation were identified, some of them showing different expression profiles between drought-tolerant and susceptible clones. Based on the obtained results, it can be concluded that factors involved a complex network of responses probably involving the abscisic signalling pathway and nitric oxide are major molecular determinants that might explain the better efficiency in controlling stomata closure and transpiration displayed by drought-tolerant clones of C. canephora. MenosThe aim of this study was to investigate the molecular mechanisms underlying drought acclimation in coffee plants by the identification of candidate genes (CGs) using different approaches. The first approach used the data generated during the Brazilian Coffee expressed sequence tag (EST) project to select 13 CGs by an in silico analysis (electronic northern). The second approach was based on screening macroarrays spotted with plasmid DNA (coffee ESTs) with separate hybridizations using leaf cDNA probes from drought-tolerant and susceptible clones of Coffea canephora var. Conilon, grown under different water regimes. This allowed the isolation of seven additional CGs. The third approach used two-dimensional gel electrophoresis to identify proteins displaying differential accumulation in leaves of drought-tolerant and susceptible clones of C. canephora. Six of them were characterized by MALDI-TOF-MS/MS (matrix-assisted laser desorption-time of flight-tandem mass spectrometry) and the corresponding proteins were identified. Finally, additional CGs were selected from the literature, and quantitative real-time polymerase chain reaction (qPCR) was performed to analyse the expression of all identified CGs. Altogether, >40 genes presenting differential gene expression during drought acclimation were identified, some of them showing different expression profiles between drought-tolerant and susceptible clones. Based on the obtained results, it can be concluded that factors involved a... Mostrar Tudo |
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Palavras-Chave: |
Candidate gene; Coffea canephora; Differential expression; Drought acclimation; Genética; Proteomic; Proteômica; Real time PCR. |
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Thesagro: |
Café; Coffea canephora; Genetics. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 03034naa a2200445 a 4500
001 1004907
005 2015-01-13
008 2012 bl uuuu u00u1 u #d
100 1 $aMARRACCINI, P.
245 $aDifferentially expressed genes and proteins upon drought acclimation in tolerant and sensitive genotypes of Coffea canephora.$h[electronic resource]
260 $c2012
520 $aThe aim of this study was to investigate the molecular mechanisms underlying drought acclimation in coffee plants by the identification of candidate genes (CGs) using different approaches. The first approach used the data generated during the Brazilian Coffee expressed sequence tag (EST) project to select 13 CGs by an in silico analysis (electronic northern). The second approach was based on screening macroarrays spotted with plasmid DNA (coffee ESTs) with separate hybridizations using leaf cDNA probes from drought-tolerant and susceptible clones of Coffea canephora var. Conilon, grown under different water regimes. This allowed the isolation of seven additional CGs. The third approach used two-dimensional gel electrophoresis to identify proteins displaying differential accumulation in leaves of drought-tolerant and susceptible clones of C. canephora. Six of them were characterized by MALDI-TOF-MS/MS (matrix-assisted laser desorption-time of flight-tandem mass spectrometry) and the corresponding proteins were identified. Finally, additional CGs were selected from the literature, and quantitative real-time polymerase chain reaction (qPCR) was performed to analyse the expression of all identified CGs. Altogether, >40 genes presenting differential gene expression during drought acclimation were identified, some of them showing different expression profiles between drought-tolerant and susceptible clones. Based on the obtained results, it can be concluded that factors involved a complex network of responses probably involving the abscisic signalling pathway and nitric oxide are major molecular determinants that might explain the better efficiency in controlling stomata closure and transpiration displayed by drought-tolerant clones of C. canephora.
650 $aCafé
650 $aCoffea canephora
650 $aGenetics
653 $aCandidate gene
653 $aCoffea canephora
653 $aDifferential expression
653 $aDrought acclimation
653 $aGenética
653 $aProteomic
653 $aProteômica
653 $aReal time PCR
700 1 $aVINECKY, F.
700 1 $aALVES, G. S. C.
700 1 $aRAMOS, H. J. O.
700 1 $aELBELT, S.
700 1 $aVIEIRA, N. G.
700 1 $aCARNEIRO, F. A.
700 1 $aSUJII, P. S.
700 1 $aALEKCEVETCH, J. C.
700 1 $aSILVA, V. A.
700 1 $aDaMATTA, F. M.
700 1 $aFERRÃO, M. A. G.
700 1 $aLEROY, T.
700 1 $aPOT, D.
700 1 $aVIEIRA, L. G. E.
700 1 $aSILVA, F. R. da
700 1 $aANDRADE, A. C.
773 $tJournal of Experimental Botany, Oxford$gv. 63, n. 11, p. 4191-4212, 2012.
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Registro original: |
Biblioteca Rui Tendinha (BRT) |
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| Acesso ao texto completo restrito à biblioteca da Biblioteca Rui Tendinha. Para informações adicionais entre em contato com biblioteca@incaper.es.gov.br. |
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Registro Completo |
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Biblioteca(s): |
Biblioteca Rui Tendinha. |
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Data corrente: |
23/06/2025 |
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Data da última atualização: |
23/06/2025 |
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Autoria: |
VENTURA, J. A.; SALES, M. D. C.; FERNANDES, P. M. B. |
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Afiliação: |
Jose Aires Ventura, Incaper; Maria Diana Cerqueira Sales; PATRÍCIA MACHADO BUENO FERNANDES. |
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Título: |
Biobase autoestimulavel fitosanitizante, processo para producao de biobase e usos da mesma. |
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Complemento do título: |
Titular(es): Universidade Federal do Espirito Santo; Incaper. |
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Ano de publicação: |
2013 |
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Fonte/Imprenta: |
Patente de Invenção, nº depósito: BR 10 2013 015300-1 A2, classificação, A01N65/42; A01N65/20; A01P1/00; A61 K36/8962; A61 K36/484; A61 K36/886; A61 P31/04, depósito em: 10 dez. 2019, concessão em: 18 jun. 2013. |
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Idioma: |
Português |
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Conteúdo: |
A presente invenção refere-se à formulação de uma biobase concentrada autoemulsionável, seu processo de produção e seus usos. A biobase é caracterizada pela aditívação de
adjuvantes técnicos tensoativos emulsificantes e surfactantes biodegradáveis e incorporação de insumos Os bioativos 100% naturais, de forma isolada ou composta, sem a aditação de conservantes químicos. Os bioativos, extraídos de espécies biologicamente ativas, são adicionados em quantidade sinergísticamente eficaz, para alcançar a atividade pretendida. O processo para a produção da biobase caracteriza-se por dispersão mecânica, composta de um sistema emulsificável surfactante, caracterizado pela combinação de tensoativos. A presente invenção confere às formulações concentradas e que podem ser diluídas, uma composição para uso pessoal, domiciliar, agricultura ou industrial |
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Thesagro: |
Patente. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01525nam a2200145 a 4500
001 1026328
005 2025-06-23
008 2013 bl uuuu j 00u1 u #d
100 1 $aVENTURA, J. A.
245 $aBiobase autoestimulavel fitosanitizante, processo para producao de biobase e usos da mesma.$h[electronic resource]
260 $aPatente de Invenção, nº depósito: BR 10 2013 015300-1 A2, classificação, A01N65/42; A01N65/20; A01P1/00; A61 K36/8962; A61 K36/484; A61 K36/886; A61 P31/04, depósito em: 10 dez. 2019, concessão em: 18 jun. 2013.$c2013
520 $aA presente invenção refere-se à formulação de uma biobase concentrada autoemulsionável, seu processo de produção e seus usos. A biobase é caracterizada pela aditívação de adjuvantes técnicos tensoativos emulsificantes e surfactantes biodegradáveis e incorporação de insumos Os bioativos 100% naturais, de forma isolada ou composta, sem a aditação de conservantes químicos. Os bioativos, extraídos de espécies biologicamente ativas, são adicionados em quantidade sinergísticamente eficaz, para alcançar a atividade pretendida. O processo para a produção da biobase caracteriza-se por dispersão mecânica, composta de um sistema emulsificável surfactante, caracterizado pela combinação de tensoativos. A presente invenção confere às formulações concentradas e que podem ser diluídas, uma composição para uso pessoal, domiciliar, agricultura ou industrial
650 $aPatente
700 1 $aSALES, M. D. C.
700 1 $aFERNANDES, P. M. B.
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