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 | Acesso ao texto completo restrito à biblioteca da Biblioteca Rui Tendinha. Para informações adicionais entre em contato com biblioteca@incaper.es.gov.br. |
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Registro Completo |
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Biblioteca(s): |
Biblioteca Rui Tendinha. |
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Data corrente: |
13/01/2015 |
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Data da última atualização: |
13/01/2015 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
MARRACCINI, P.; VINECKY, F.; ALVES, G. S. C.; RAMOS, H. J. O.; ELBELT, S.; VIEIRA, N. G.; CARNEIRO, F. A.; SUJII, P. S.; ALEKCEVETCH, J. C.; SILVA, V. A.; DaMATTA, F. M.; FERRÃO, M. A. G.; LEROY, T.; POT, D.; VIEIRA, L. G. E.; SILVA, F. R. da; ANDRADE, A. C. |
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Afiliação: |
Maria Amélia Gava Ferrão, Incaper/Embrapa Café. |
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Título: |
Differentially expressed genes and proteins upon drought acclimation in tolerant and sensitive genotypes of Coffea canephora. |
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Ano de publicação: |
2012 |
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Fonte/Imprenta: |
Journal of Experimental Botany, Oxford v. 63, n. 11, p. 4191-4212, 2012. |
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Idioma: |
Inglês |
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Conteúdo: |
The aim of this study was to investigate the molecular mechanisms underlying drought acclimation in coffee plants by the identification of candidate genes (CGs) using different approaches. The first approach used the data generated during the Brazilian Coffee expressed sequence tag (EST) project to select 13 CGs by an in silico analysis (electronic northern). The second approach was based on screening macroarrays spotted with plasmid DNA (coffee ESTs) with separate hybridizations using leaf cDNA probes from drought-tolerant and susceptible clones of Coffea canephora var. Conilon, grown under different water regimes. This allowed the isolation of seven additional CGs. The third approach used two-dimensional gel electrophoresis to identify proteins displaying differential accumulation in leaves of drought-tolerant and susceptible clones of C. canephora. Six of them were characterized by MALDI-TOF-MS/MS (matrix-assisted laser desorption-time of flight-tandem mass spectrometry) and the corresponding proteins were identified. Finally, additional CGs were selected from the literature, and quantitative real-time polymerase chain reaction (qPCR) was performed to analyse the expression of all identified CGs. Altogether, >40 genes presenting differential gene expression during drought acclimation were identified, some of them showing different expression profiles between drought-tolerant and susceptible clones. Based on the obtained results, it can be concluded that factors involved a complex network of responses probably involving the abscisic signalling pathway and nitric oxide are major molecular determinants that might explain the better efficiency in controlling stomata closure and transpiration displayed by drought-tolerant clones of C. canephora. MenosThe aim of this study was to investigate the molecular mechanisms underlying drought acclimation in coffee plants by the identification of candidate genes (CGs) using different approaches. The first approach used the data generated during the Brazilian Coffee expressed sequence tag (EST) project to select 13 CGs by an in silico analysis (electronic northern). The second approach was based on screening macroarrays spotted with plasmid DNA (coffee ESTs) with separate hybridizations using leaf cDNA probes from drought-tolerant and susceptible clones of Coffea canephora var. Conilon, grown under different water regimes. This allowed the isolation of seven additional CGs. The third approach used two-dimensional gel electrophoresis to identify proteins displaying differential accumulation in leaves of drought-tolerant and susceptible clones of C. canephora. Six of them were characterized by MALDI-TOF-MS/MS (matrix-assisted laser desorption-time of flight-tandem mass spectrometry) and the corresponding proteins were identified. Finally, additional CGs were selected from the literature, and quantitative real-time polymerase chain reaction (qPCR) was performed to analyse the expression of all identified CGs. Altogether, >40 genes presenting differential gene expression during drought acclimation were identified, some of them showing different expression profiles between drought-tolerant and susceptible clones. Based on the obtained results, it can be concluded that factors involved a... Mostrar Tudo |
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Palavras-Chave: |
Candidate gene; Coffea canephora; Differential expression; Drought acclimation; Genética; Proteomic; Proteômica; Real time PCR. |
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Thesagro: |
Café; Coffea canephora; Genetics. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 03034naa a2200445 a 4500
001 1004907
005 2015-01-13
008 2012 bl uuuu u00u1 u #d
100 1 $aMARRACCINI, P.
245 $aDifferentially expressed genes and proteins upon drought acclimation in tolerant and sensitive genotypes of Coffea canephora.$h[electronic resource]
260 $c2012
520 $aThe aim of this study was to investigate the molecular mechanisms underlying drought acclimation in coffee plants by the identification of candidate genes (CGs) using different approaches. The first approach used the data generated during the Brazilian Coffee expressed sequence tag (EST) project to select 13 CGs by an in silico analysis (electronic northern). The second approach was based on screening macroarrays spotted with plasmid DNA (coffee ESTs) with separate hybridizations using leaf cDNA probes from drought-tolerant and susceptible clones of Coffea canephora var. Conilon, grown under different water regimes. This allowed the isolation of seven additional CGs. The third approach used two-dimensional gel electrophoresis to identify proteins displaying differential accumulation in leaves of drought-tolerant and susceptible clones of C. canephora. Six of them were characterized by MALDI-TOF-MS/MS (matrix-assisted laser desorption-time of flight-tandem mass spectrometry) and the corresponding proteins were identified. Finally, additional CGs were selected from the literature, and quantitative real-time polymerase chain reaction (qPCR) was performed to analyse the expression of all identified CGs. Altogether, >40 genes presenting differential gene expression during drought acclimation were identified, some of them showing different expression profiles between drought-tolerant and susceptible clones. Based on the obtained results, it can be concluded that factors involved a complex network of responses probably involving the abscisic signalling pathway and nitric oxide are major molecular determinants that might explain the better efficiency in controlling stomata closure and transpiration displayed by drought-tolerant clones of C. canephora.
650 $aCafé
650 $aCoffea canephora
650 $aGenetics
653 $aCandidate gene
653 $aCoffea canephora
653 $aDifferential expression
653 $aDrought acclimation
653 $aGenética
653 $aProteomic
653 $aProteômica
653 $aReal time PCR
700 1 $aVINECKY, F.
700 1 $aALVES, G. S. C.
700 1 $aRAMOS, H. J. O.
700 1 $aELBELT, S.
700 1 $aVIEIRA, N. G.
700 1 $aCARNEIRO, F. A.
700 1 $aSUJII, P. S.
700 1 $aALEKCEVETCH, J. C.
700 1 $aSILVA, V. A.
700 1 $aDaMATTA, F. M.
700 1 $aFERRÃO, M. A. G.
700 1 $aLEROY, T.
700 1 $aPOT, D.
700 1 $aVIEIRA, L. G. E.
700 1 $aSILVA, F. R. da
700 1 $aANDRADE, A. C.
773 $tJournal of Experimental Botany, Oxford$gv. 63, n. 11, p. 4191-4212, 2012.
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Biblioteca Rui Tendinha (BRT) |
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| Acesso ao texto completo restrito à biblioteca da Biblioteca Rui Tendinha. Para informações adicionais entre em contato com biblioteca@incaper.es.gov.br. |
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Registro Completo |
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Biblioteca(s): |
Biblioteca Rui Tendinha. |
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Data corrente: |
01/08/2025 |
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Data da última atualização: |
01/08/2025 |
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Tipo da produção científica: |
Publicação em Anais de Congresso |
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Autoria: |
OLIVEIRA, A. C. de; SILVA, A. M. da.; SIQUEIRA, A. P.; COSLOP, R. L. A.; COSTA, A. L. |
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Afiliação: |
Angélica Carvalhais de Oliveira, Incaper; Alessandra Maria da Silva, UFCA; Ana Paula de Oliveira Siqueira, Incaper; Rogelielder Luiz Arpini Coslop; Abel Lopes Costa, Incaper. |
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Título: |
Relato de experiencia do projeto Cadernetas Agroecológicas Capixabas com pescadoras de Itapemirim-ES : impactos socioeconomicos e ambientais sob uma perspectiva feminista e agroecologica. |
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Ano de publicação: |
2025 |
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Fonte/Imprenta: |
In: SIMPOSIO INCAPER PESQUISA, 4., Seminário de Iniciação Científica do Incaper, 4., 2024, Vitoria. Desafios e potencialidades para a agropecuária, pesquisa e extensão na era da automação e inteligência artificial. Vitoria, ES : Incaper, 2025. p. 96. Editores: Andrea Ferreira da Costa, Jose Salazar Zanuncio Junior e Renato Correa Taques. |
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Idioma: |
Português |
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Palavras-Chave: |
Agroecologia; Metodologias de ATER. |
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Thesagro: |
Igualdade de Genero; Mulher; Mulher Rural. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01036nam a2200205 a 4500
001 1026571
005 2025-08-01
008 2025 bl uuuu u01u1 u #d
100 1 $aOLIVEIRA, A. C. de
245 $aRelato de experiencia do projeto Cadernetas Agroecológicas Capixabas com pescadoras de Itapemirim-ES$bimpactos socioeconomicos e ambientais sob uma perspectiva feminista e agroecologica.$h[electronic resource]
260 $aIn: SIMPOSIO INCAPER PESQUISA, 4., Seminário de Iniciação Científica do Incaper, 4., 2024, Vitoria. Desafios e potencialidades para a agropecuária, pesquisa e extensão na era da automação e inteligência artificial. Vitoria, ES : Incaper, 2025. p. 96. Editores: Andrea Ferreira da Costa, Jose Salazar Zanuncio Junior e Renato Correa Taques.$c2025
650 $aIgualdade de Genero
650 $aMulher
650 $aMulher Rural
653 $aAgroecologia
653 $aMetodologias de ATER
700 1 $aSILVA, A. M. da.
700 1 $aSIQUEIRA, A. P.
700 1 $aCOSLOP, R. L. A.
700 1 $aCOSTA, A. L.
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