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1. | | PERON, F. N.; FERNANDES, P. M. B.; VENTURA, J. A. Detecção do pineapple mealybug wilt-associated virus (PMWaV1, PMWaV2 e PMWaV3) em abacaxizeiros no Estado do Espírito Santo. Tropical Plant Pathology, Brasília, DF, v. 35, p. S185, ago. 2010. Suplemento, ref. 05.091. Edição dos resumos do XLIII Congresso Brasileiro de Fitopatologia, Cuiabá, ago. 2010.Biblioteca(s): Biblioteca Rui Tendinha. |
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3. | | COSTA, H. B.; FERNANDES, P.M.B.; VENTURA, J. A. Extração, purificação parcial e comparação de duas técnicas de precipitação de enzimas proteolíticas do abacaxizeiro. In: CONGRESSO BRASILEIRO DE FRUTICULTURA, 20.; ANNUAL MEETING OF THE INTERAMERICAN SOCIETY FOR TROPICAL HORTICULTURE, 54., 2008, Vitória. Frutas para todos: estratégias, tecnologias e visão sustentável: anais. Vitória: INCAPER: Sociedade Brasileira de Fruticultura, 2008. 5p.Biblioteca(s): Biblioteca Rui Tendinha. |
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4. | | RODRIGUES, S. P.; VENTURA, J. A.; ZINGALI, R. B.; FERNANDES, P. M. B. Avaliação de diferentes métodos de preparação de amostra de proteínas de folha de mamoeiro para análise proteômica por eletroforese bi-dimensional. In: CONGRESSO BRASILEIRO DE FRUTICULTURA, 20.; ANNUAL MEETING OF THE INTERAMERICAN SOCIETY FOR TROPICAL HORTICULTURE, 54., 2008, Vitória. Frutas para todos: estratégias, tecnologias e visão sustentável: anais. Vitória: INCAPER: Sociedade Brasileira de Fruticultura, 2008. 5p.Biblioteca(s): Biblioteca Rui Tendinha. |
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6. | | RODRIGUES, S. P.; CUNHA, M. da.; VENTURA, J. A.; FERNANDES, P. M. B. Análise estrutural e histoquímica de compostos presentes no latex de plantas com meleira e sua relação com o Papaya meleira virus (PMev). In: SIMPÓSIO PAPAYA BRASILEIRO, 2., 2005, Vitória, ES. MARTINS, D dos S. (Ed.). Papaya Brasil : mercado e inovações tecnológicas para o mamão. Vitória: Incaper, 2005. p. 430-433.Biblioteca(s): Biblioteca Rui Tendinha. |
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10. | | QUADROS, O. de F.; VENTURA, J. A.; FERNANDES, A. A. R.; FERNANDES, P. M. B. Atividade fungicida e larvicida de extrato proteico de folhas de mamoeiro infectadas pelo vírus da meleira. In: SIMPÓSIO DO PAPAYA BRASILEIRO, 6., 2015, Vitória, ES. Tecnologia de produção e mercado para o mamão brasileiro : Anais... Vitória, ES : Incaper, 2015.Biblioteca(s): Biblioteca Rui Tendinha. |
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12. | | CARNIELLI, L.; FERNANDES, P. M. B.; FERNANDES, A. A. R.; VENTURA, J. A. Diagnóstico molecular de Fusarium guttiforme com PCR em tempo real. In: CONGRESSO BRASILEIRO DE FITOPATOLOGIA, 48.; CONGRESSO BRASILEIRO DE PATOLOGIA PÓS-COLHEITA, 2., 2015, São Pedro, SP. Fitopatologia de precisão: fronteiras da ciência: anais. São Pedro, SP: Sociedade Brasileira de Fitopatologia, 2015.Biblioteca(s): Biblioteca Rui Tendinha. |
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14. | | DETONI, J. L.; KORRES, A. M. N.; VENTURA, J. A.; FERNANDES, P. M. B. Estudo de enzimas proteolíticas e pectinolíticas relacionadas a microrganismos associados à podridão espumosa do abacaxi. In: CONGRESSO BRASILEIRO DE FRUTICULTURA, 20.; ANNUAL MEETING OF THE INTERAMERICAN SOCIETY FOR TROPICAL HORTICULTURE, 54., 2008, Vitória. Frutas para todos: estratégias, tecnologias e visão sustentável: anais. Vitória: INCAPER: Sociedade Brasileira de Fruticultura, 2008. 5p.Biblioteca(s): Biblioteca Rui Tendinha. |
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16. | | SILVA, W. P. da; QUADROS, O. de F.; FERNANDES, P. M. B.; VENTURA, J. A. Pré-aclimatação de plantas transformadas por CRISPR/CAS9 para deleção do gene ?-1,3-glucanase e por gateway para superexpressão da proteína Quitinase em Carica Papaya L. In: SIMPÓSIO DO PAPAYA BRASILEIRO, 8. , Linhares, ES. Papaya Brasil : produção e sustentabilidade. Organizadores, David dos Santos Martins e José Aires Ventura. Vitória, ES : Incaper, 2022. Anais... Linhares, ES : Incaper, p. 204-209, 2022.Biblioteca(s): Biblioteca Rui Tendinha. |
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17. | | COSTA, H. B. da.; VENTURA, J. A.; FERNANDES, P. M. B. Processo de purificação de bromelina, bromelina purificada e usos da enzima purificada. Titular: Instituto Capixaba de Pesquisa, Assistência Técnica e Extensão Rural; Universidade Federal do Espírito Santo. Patente de Invenção, nº depósito: BR 10 2012 027122-2 A2, classificação, A61 K36/88; A61 K38/48; A61K125/00, depósito em: 23 out. 2012, concessão em: 10 març. 2015.Biblioteca(s): Biblioteca Rui Tendinha. |
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Registros recuperados : 65 | |
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Registro Completo |
Biblioteca(s): |
Biblioteca Rui Tendinha. |
Data corrente: |
25/09/2014 |
Data da última atualização: |
01/09/2015 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 1 |
Autoria: |
ABREU, P. M. V.; GASPAR, C. G.; BUSS, D. S.; VENTURA, J. A.; FERREIRA, P. C. G.; FERNANDES, P. M. B. |
Afiliação: |
Paolla M. V. Abreu, Universidade Federal do Espíríto Santo; Clicia G. Gaspar, Univervidade Federal do Rio de Janeiro; David S. Buss, Universidade Federal do Espírito Santo; Jose Aires Ventura, Incaper; Paulo C. G. Ferreira, Universidade Federal do Rio de Janeiro; Patricia M. B. Fernandes, Universidade Federal do Espírito Santo. |
Título: |
Carica papaya MicroRNAs Are Responsive to Papaya meleira virus Infection. |
Ano de publicação: |
2014 |
Fonte/Imprenta: |
PLOS ONE, v. 9, issue 7, p. 1-13, july 2014. |
Páginas: |
13 p. |
Descrição Física: |
il., color. |
ISSN: |
1932-6203 |
DOI: |
10.1371 |
Idioma: |
Português |
Conteúdo: |
MicroRNAs are implicated in the response to biotic stresses. Papaya meleira virus (PMeV) is the causal agent of sticky disease, a commercially important pathology in papaya for which there are currently no resistant varieties. PMeV has a number of unusual features, such as residence in the laticifers of infected plants, and the response of the papaya to PMeV infection is not well understood. The protein levels of 20S proteasome subunits increase during PMeV infection, suggesting that proteolysis could be an important aspect of the plant defense response mechanism. To date, 10,598 plant microRNAs have been identified in the Plant miRNAs Database, but only two, miR162 and miR403, are from papaya. In this study, known plant microRNA sequences were used to search for potential microRNAs in the papaya genome. A total of 462 microRNAs, representing 72 microRNA families, were identified. The expression of 11 microRNAs, whose targets are involved in 20S and 26S proteasomal degradation and in other stress response pathways, was compared by real-time PCR in healthy and infected papaya leaf tissue. We found that the expression of miRNAs involved in proteasomal degradation increased in response to very low levels of PMeV titre and decreased as the viral titre increased. In contrast, miRNAs implicated in the plant response to biotic stress decreased their expression at very low level of PMeV and increased at high PMeV levels. Corroborating with this results, analysed target genes for this miRNAs had their expression modulated in a dependent manner. This study represents a comprehensive identification of conserved miRNAs inpapaya. The data presented here might help to complement the available molecular and genomic tools for the study of papaya. The differential expression of some miRNAs and identifying their target genes will be helpful for understanding the regulation and interaction of PMeV and papaya. MenosMicroRNAs are implicated in the response to biotic stresses. Papaya meleira virus (PMeV) is the causal agent of sticky disease, a commercially important pathology in papaya for which there are currently no resistant varieties. PMeV has a number of unusual features, such as residence in the laticifers of infected plants, and the response of the papaya to PMeV infection is not well understood. The protein levels of 20S proteasome subunits increase during PMeV infection, suggesting that proteolysis could be an important aspect of the plant defense response mechanism. To date, 10,598 plant microRNAs have been identified in the Plant miRNAs Database, but only two, miR162 and miR403, are from papaya. In this study, known plant microRNA sequences were used to search for potential microRNAs in the papaya genome. A total of 462 microRNAs, representing 72 microRNA families, were identified. The expression of 11 microRNAs, whose targets are involved in 20S and 26S proteasomal degradation and in other stress response pathways, was compared by real-time PCR in healthy and infected papaya leaf tissue. We found that the expression of miRNAs involved in proteasomal degradation increased in response to very low levels of PMeV titre and decreased as the viral titre increased. In contrast, miRNAs implicated in the plant response to biotic stress decreased their expression at very low level of PMeV and increased at high PMeV levels. Corroborating with this results, analysed target genes for thi... Mostrar Tudo |
Palavras-Chave: |
Carica papaya; Doenças; Mamão; Meleira; MiRNAs; PMeV; Vírus. |
Thesaurus NAL: |
Carica papaya; Disease; Meleira; MiRNAs; PMeV; Virus. |
Categoria do assunto: |
-- |
URL: |
http://biblioteca.incaper.es.gov.br/digital/bitstream/item/397/1/Carica-papaya-microRNAS-Are-responsive-to-papaya.pdf
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Marc: |
LEADER 02852naa a2200373 a 4500 001 1004325 005 2015-09-01 008 2014 bl uuuu u00u1 u #d 022 $a1932-6203 024 7 $a10.1371$2DOI 100 1 $aABREU, P. M. V. 245 $aCarica papaya MicroRNAs Are Responsive to Papaya meleira virus Infection.$h[electronic resource] 260 $c2014 300 $a13 p.$cil., color. 520 $aMicroRNAs are implicated in the response to biotic stresses. Papaya meleira virus (PMeV) is the causal agent of sticky disease, a commercially important pathology in papaya for which there are currently no resistant varieties. PMeV has a number of unusual features, such as residence in the laticifers of infected plants, and the response of the papaya to PMeV infection is not well understood. The protein levels of 20S proteasome subunits increase during PMeV infection, suggesting that proteolysis could be an important aspect of the plant defense response mechanism. To date, 10,598 plant microRNAs have been identified in the Plant miRNAs Database, but only two, miR162 and miR403, are from papaya. In this study, known plant microRNA sequences were used to search for potential microRNAs in the papaya genome. A total of 462 microRNAs, representing 72 microRNA families, were identified. The expression of 11 microRNAs, whose targets are involved in 20S and 26S proteasomal degradation and in other stress response pathways, was compared by real-time PCR in healthy and infected papaya leaf tissue. We found that the expression of miRNAs involved in proteasomal degradation increased in response to very low levels of PMeV titre and decreased as the viral titre increased. In contrast, miRNAs implicated in the plant response to biotic stress decreased their expression at very low level of PMeV and increased at high PMeV levels. Corroborating with this results, analysed target genes for this miRNAs had their expression modulated in a dependent manner. This study represents a comprehensive identification of conserved miRNAs inpapaya. The data presented here might help to complement the available molecular and genomic tools for the study of papaya. The differential expression of some miRNAs and identifying their target genes will be helpful for understanding the regulation and interaction of PMeV and papaya. 650 $aCarica papaya 650 $aDisease 650 $aMeleira 650 $aMiRNAs 650 $aPMeV 650 $aVirus 653 $aCarica papaya 653 $aDoenças 653 $aMamão 653 $aMeleira 653 $aMiRNAs 653 $aPMeV 653 $aVírus 700 1 $aGASPAR, C. G. 700 1 $aBUSS, D. S. 700 1 $aVENTURA, J. A. 700 1 $aFERREIRA, P. C. G. 700 1 $aFERNANDES, P. M. B. 773 $tPLOS ONE$gv. 9, issue 7, p. 1-13, july 2014.
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