|
|
Registro Completo |
Biblioteca(s): |
Biblioteca Rui Tendinha. |
Data corrente: |
02/12/2019 |
Data da última atualização: |
02/12/2019 |
Tipo da produção científica: |
Folheto/Folha |
Autoria: |
Incaper. Coordenação de Meteorologia. |
Título: |
Informativo Climático Mensal do Espírito Santo - outubro de 2019. |
Ano de publicação: |
2019 |
Fonte/Imprenta: |
Vitória, ES, ano. 1, n. 10, outubro 2019. |
Páginas: |
2 p. |
Idioma: |
Português |
Notas: |
Esta publicação foi concebida em dois formatos, ePUB e PDF. |
Conteúdo: |
Em outubro de 2019, poucos sistemas influenciaram as condições de tempo no Estado, que teve dias predominantemente de sol com altas temperaturas... |
Palavras-Chave: |
Chuva; Clima; Clima capixaba; Espírito Santo (Estado). |
Thesagro: |
Balanço Hídrico; Hidrometeorologia; Precipitação pluvial; Previsão do Tempo; Temperatura. |
Categoria do assunto: |
J Meteorologia |
URL: |
https://biblioteca.incaper.es.gov.br/digital/bitstream/123456789/3945/1/Informativo-Climatico-Mensal-ES-ano1-n10-outubro2019-Incaper.pdf
https://biblioteca.incaper.es.gov.br/digital/bitstream/123456789/3946/1/Informativo-Climatico-Mensal-ES-ano1-n10-outubro2019-Incaper.epub
|
Marc: |
LEADER 00969nam a2200241 a 4500 001 1021980 005 2019-12-02 008 2019 bl uuuu u0uu1 u #d 100 1 $aIncaper. Coordenação de Meteorologia. 245 $aInformativo Climático Mensal do Espírito Santo - outubro de 2019.$h[electronic resource] 260 $aVitória, ES, ano. 1, n. 10, outubro 2019.$c2019 300 $a2 p. 500 $aEsta publicação foi concebida em dois formatos, ePUB e PDF. 520 $aEm outubro de 2019, poucos sistemas influenciaram as condições de tempo no Estado, que teve dias predominantemente de sol com altas temperaturas... 650 $aBalanço Hídrico 650 $aHidrometeorologia 650 $aPrecipitação pluvial 650 $aPrevisão do Tempo 650 $aTemperatura 653 $aChuva 653 $aClima 653 $aClima capixaba 653 $aEspírito Santo (Estado)
Download
Esconder MarcMostrar Marc Completo |
Registro original: |
Biblioteca Rui Tendinha (BRT) |
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
|
Registro Completo |
Biblioteca(s): |
Biblioteca Rui Tendinha. |
Data corrente: |
09/09/2020 |
Data da última atualização: |
09/09/2020 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 1 |
Autoria: |
DUARTE, M. F.; PEREIRA-CARVALHO, R. de C.; REIS, L. de N. A. dos; ROJAS, M. J.; GILBERTSON, R.; COSTA, H.; BOITEUX, L. S.; FONSECA, M. E. N. |
Afiliação: |
Macaria Ferreira Duarte, UnB; Rita de Cássia Pereira Carvalho, UnB; Luciane de Nazaré Almeida dos Reis, UnB; Maria J. Rojas, UC-DAVIS; Robert Gilbertson, UC-DAVIS; Helcio Costa, Incaper; Leonardo Silva Boiteux, EMBRAPA; Maria Esther N. Fonseca, EMBRAPA. |
Título: |
Natural Infection of Tomatoes (Solanum lycopersicum) by Euphorbia yellow mosaic virus Isolates across Four Brazilian States. |
Ano de publicação: |
2020 |
Fonte/Imprenta: |
Plant Disease, set. 2020. |
Idioma: |
Inglês |
Conteúdo: |
Severe yield losses induced by a complex of whitefly?transmitted Begomovirus species (family Geminiviridae) have been reported in tomatoes in Brazil (Reis et al. 2020). Nine isolates were obtained from tomato plants exhibiting begomovirus?like symptoms (viz. apical and interveinal chlorosis, yellow spots, and stunting) during independent field surveys: one isolate in Sumaré, São Paulo?SP State (isolate SP?066) in 2001, two in Serra Negra, Minas Gerais?MG (MG?012 and MG?016) in 2002, five in Caxias do Sul, Rio Grande do Sul?RS (RS?039, RS?045, RS?046, RS?047 and RS?058) in 2011 and one in Domingos Martins, Espírito Santo?ES (ES?148) in 2016. Disease incidence across all sampled fields ranged from 30% (in Domingos Martins?ES) to 90% in Sumaré?SP. Total DNA extraction was done by a modified CTAB method (Boiteux et al., 1999). Begomovirus infection was confirmed in all isolates by selective amplification of viral DNA?A segments using the primer pairs ?PAL1v1978 / PAR1c496? (Rojas et al., 1993) and ?BegomoAFor1? / ?BegomoARev1? (Ha et al., 2006), which produce two large and non?overlapping segments (≈1120 bp and ≈1205 bp, respectively). These PCR amplicons were initially characterized via direct Sanger dideoxy sequencing at CNPH. BLASTn analysis of the partial DNA?A genomes of these nine isolates indicated identity levels of 95?97% to three euphorbia yellow mosaic virus (EuYMV) reference isolates (= KY559532, JF756674, and KY559583) found infecting the weed Euphorbia heterophylla L. The entire DNA?A (2,609 nts = MN746971) and DNA?B (2,579 nts = MN746970) components of the MG?016 isolate were obtained via high?performance sequencing using Illumina HiSeq 2500 system (Macrogen Inc., South Korea). Sequences were assembled with the CLC Genomics Workbench program 10. Contigs were validated by BLASTx and BLASTn and compared to the ssDNA virus database at NCBI (www.ncbi.nlm.nih.gov). The fully?characterized MG?016 isolate displayed identity levels ranging from 97 to 99% to the EuYMV reference isolates as well as similar genomic features such as the conserved TATA box, nonanucleotide, and iterons (that were in agreement with a cognate nature of the DNA?A and DNA?B components). A partial sequence of the DNA?B genome was also obtained for the MG?012 isolate (MT7831942). The isolates MG?012 and MG?016 were found in mixed infections with tomato severe rugose virus (ToSRV) and tomato golden vein virus (TGVV), respectively. In addition, the complete DNA?A genomes of ES?148 (MN746972) and SP?066 (MN782438) were also obtained via a combination of primer walking and Sanger dideoxy sequencing, displaying 96?98% identity to EuYMV isolates. To our knowledge, this is the first report of multiple and independent events of natural infection of tomatoes by EuYMV isolates. Our results confirm the natural host status of tomatoes to EuYMV isolates as indicated in previous infectivity assays using biolistic inoculation (Barreto et al., 2013). The weed E. heterophylla is widely disseminated and very often present within tomato fields due to its higher levels of tolerance to the major herbicide (metribuzin) employed in this crop. Therefore, this weed may act as a persistent reservoir of tomato?infecting EuYMV isolates, which may allow the selection of viral populations potentially more adapted to this vegetable crop. MenosSevere yield losses induced by a complex of whitefly?transmitted Begomovirus species (family Geminiviridae) have been reported in tomatoes in Brazil (Reis et al. 2020). Nine isolates were obtained from tomato plants exhibiting begomovirus?like symptoms (viz. apical and interveinal chlorosis, yellow spots, and stunting) during independent field surveys: one isolate in Sumaré, São Paulo?SP State (isolate SP?066) in 2001, two in Serra Negra, Minas Gerais?MG (MG?012 and MG?016) in 2002, five in Caxias do Sul, Rio Grande do Sul?RS (RS?039, RS?045, RS?046, RS?047 and RS?058) in 2011 and one in Domingos Martins, Espírito Santo?ES (ES?148) in 2016. Disease incidence across all sampled fields ranged from 30% (in Domingos Martins?ES) to 90% in Sumaré?SP. Total DNA extraction was done by a modified CTAB method (Boiteux et al., 1999). Begomovirus infection was confirmed in all isolates by selective amplification of viral DNA?A segments using the primer pairs ?PAL1v1978 / PAR1c496? (Rojas et al., 1993) and ?BegomoAFor1? / ?BegomoARev1? (Ha et al., 2006), which produce two large and non?overlapping segments (≈1120 bp and ≈1205 bp, respectively). These PCR amplicons were initially characterized via direct Sanger dideoxy sequencing at CNPH. BLASTn analysis of the partial DNA?A genomes of these nine isolates indicated identity levels of 95?97% to three euphorbia yellow mosaic virus (EuYMV) reference isolates (= KY559532, JF756674, and KY559583) found infecting the weed Euphorbia ... Mostrar Tudo |
Thesagro: |
Doença de Planta; Erva Daninha; Mosaico; Tomate. |
Thesaurus NAL: |
Begomovirus. |
Categoria do assunto: |
H Saúde e Patologia |
URL: |
https://biblioteca.incaper.es.gov.br/digital/bitstream/123456789/4073/1/natural-infection-tomatoes-costa.pdf
|
Marc: |
LEADER 04125naa a2200265 a 4500 001 1022277 005 2020-09-09 008 2020 bl uuuu u00u1 u #d 100 1 $aDUARTE, M. F. 245 $aNatural Infection of Tomatoes (Solanum lycopersicum) by Euphorbia yellow mosaic virus Isolates across Four Brazilian States.$h[electronic resource] 260 $c2020 520 $aSevere yield losses induced by a complex of whitefly?transmitted Begomovirus species (family Geminiviridae) have been reported in tomatoes in Brazil (Reis et al. 2020). Nine isolates were obtained from tomato plants exhibiting begomovirus?like symptoms (viz. apical and interveinal chlorosis, yellow spots, and stunting) during independent field surveys: one isolate in Sumaré, São Paulo?SP State (isolate SP?066) in 2001, two in Serra Negra, Minas Gerais?MG (MG?012 and MG?016) in 2002, five in Caxias do Sul, Rio Grande do Sul?RS (RS?039, RS?045, RS?046, RS?047 and RS?058) in 2011 and one in Domingos Martins, Espírito Santo?ES (ES?148) in 2016. Disease incidence across all sampled fields ranged from 30% (in Domingos Martins?ES) to 90% in Sumaré?SP. Total DNA extraction was done by a modified CTAB method (Boiteux et al., 1999). Begomovirus infection was confirmed in all isolates by selective amplification of viral DNA?A segments using the primer pairs ?PAL1v1978 / PAR1c496? (Rojas et al., 1993) and ?BegomoAFor1? / ?BegomoARev1? (Ha et al., 2006), which produce two large and non?overlapping segments (≈1120 bp and ≈1205 bp, respectively). These PCR amplicons were initially characterized via direct Sanger dideoxy sequencing at CNPH. BLASTn analysis of the partial DNA?A genomes of these nine isolates indicated identity levels of 95?97% to three euphorbia yellow mosaic virus (EuYMV) reference isolates (= KY559532, JF756674, and KY559583) found infecting the weed Euphorbia heterophylla L. The entire DNA?A (2,609 nts = MN746971) and DNA?B (2,579 nts = MN746970) components of the MG?016 isolate were obtained via high?performance sequencing using Illumina HiSeq 2500 system (Macrogen Inc., South Korea). Sequences were assembled with the CLC Genomics Workbench program 10. Contigs were validated by BLASTx and BLASTn and compared to the ssDNA virus database at NCBI (www.ncbi.nlm.nih.gov). The fully?characterized MG?016 isolate displayed identity levels ranging from 97 to 99% to the EuYMV reference isolates as well as similar genomic features such as the conserved TATA box, nonanucleotide, and iterons (that were in agreement with a cognate nature of the DNA?A and DNA?B components). A partial sequence of the DNA?B genome was also obtained for the MG?012 isolate (MT7831942). The isolates MG?012 and MG?016 were found in mixed infections with tomato severe rugose virus (ToSRV) and tomato golden vein virus (TGVV), respectively. In addition, the complete DNA?A genomes of ES?148 (MN746972) and SP?066 (MN782438) were also obtained via a combination of primer walking and Sanger dideoxy sequencing, displaying 96?98% identity to EuYMV isolates. To our knowledge, this is the first report of multiple and independent events of natural infection of tomatoes by EuYMV isolates. Our results confirm the natural host status of tomatoes to EuYMV isolates as indicated in previous infectivity assays using biolistic inoculation (Barreto et al., 2013). The weed E. heterophylla is widely disseminated and very often present within tomato fields due to its higher levels of tolerance to the major herbicide (metribuzin) employed in this crop. Therefore, this weed may act as a persistent reservoir of tomato?infecting EuYMV isolates, which may allow the selection of viral populations potentially more adapted to this vegetable crop. 650 $aBegomovirus 650 $aDoença de Planta 650 $aErva Daninha 650 $aMosaico 650 $aTomate 700 1 $aPEREIRA-CARVALHO, R. de C. 700 1 $aREIS, L. de N. A. dos 700 1 $aROJAS, M. J. 700 1 $aGILBERTSON, R. 700 1 $aCOSTA, H. 700 1 $aBOITEUX, L. S. 700 1 $aFONSECA, M. E. N. 773 $tPlant Disease, set. 2020.
Download
Esconder MarcMostrar Marc Completo |
Registro original: |
Biblioteca Rui Tendinha (BRT) |
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
|
Registros recuperados : 1 | |
Registros recuperados : 1 | |
|
|
|