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| 8. |  | CAVATTE, P. C.; FERRÃO, R. G.; SILVA, A. T. da; SILVA, J. A. da Uso de índices fisiológicos para identificar clones de Coffea canephora com maior tolerância a seca: simplificando metodologias para abordar um problema complexo. In: CONGRESSO CAPIXABA DE PESQUISA AGROPECUÁRIA, 1., Vitória, ES. Anais 2021 : congresso capixaba de pesquisa agropecuária [recurso eletrônico]. Vitória, ES: Incaper, 2021. color. PDF ; 25,4 MB. E-book, no formato PDF. (Incaper, Documentos, 289). Pedro Luís Pereira Teixeira de Carvalho, Carlos Henrique Rodrigues de Oliveira, José Aires Ventura, Marcos Vinicius Winckler Caldeira e Romário Gava Ferrão, editores. p. 121| Biblioteca(s): Biblioteca Rui Tendinha. |
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| 9. | | MILHEIROS, I. S.; SILVA, U. R.; SENRA, J. F. de B.; SILVA, J. A. da; ESPOSTI, M. D. Avaliação do índice de vegetação por diferença normalizada e índice SPAD em jardim clonal superadensado de Coffea Canephora. In: SIMPÓSIO INCAPER PESQUISA, 1. , Vitória, ES. Avaliação do índice de vegetação por diferença normalizada e índice SPAD em jardim clonal superadensado de Coffea Canephora. Editores, Andre Guarçoni Martins ... [et al]., Vitória, ES : Incaper, 2022. Anais... Vitória, ES : Incaper, p. 25, 2022.| Biblioteca(s): Biblioteca Rui Tendinha. |
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| 11. | | ZACARIAS, A. J.; CAETANO, L. C. S.; ESPOSTI, M. D.; CONCEIÇÃO, A. O. da; SILVA, J. A. da; SILVA, F. G. da; MILHEIROS, I. S. Avaliacao da polinizacao natural em maracujazeiro no Sul do Espirito Santo. In: ENCONTRO LATINO AMERICANO DE INICIACAO CIENTIFICA, 26.; ENCONTRO LATINO AMERICANO DE POS GRADUACAO, 22., 2023, Sao Jose dos Campos, SP. Ciencias basicas e o desenvolvimento sustentavel: a interface dos saberes para a sociedade . Sao Jose dos Campos: UNIVAP, 2022.| Biblioteca(s): Biblioteca Rui Tendinha. |
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| 13. | | SILVA, J. A. da S.; SENRA, J. F. de B.; SILVA, F. G. da; MILHEIROS, I. S.; VEIGA, J. C. da S.; ESPOSTI, M. D. Desempenho inicial de genótipos de Café Conilon cultivados em condições contrastantes de altitude. In: SIMPÓSIO INCAPER PESQUISA, 1. , Vitória, ES. Desempenho inicial de genótipos de Café Conilon cultivados em condições contrastantes de altitude. Editores, Andre Guarçoni Martins ... [et al]., Vitória, ES : Incaper, 2022. Anais... Vitória, ES : Incaper, p. 26, 2022.| Biblioteca(s): Biblioteca Rui Tendinha. |
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| 14. | | RAIMUNDO, E. G.; MILHEIROS, I. S.; SILVA, J. A. da; CONCEIÇÃO, A. O. da; SILVA, F. G. da; ESPOSTI, M. D.; GUARÇONI M., A. Influencia de fontes minerais de K na produtividade e na granulometria dos graos do cafe conilon. In: ENCONTRO LATINO AMERICANO DE INICIACAO CIENTIFICA, 26.; ENCONTRO LATINO AMERICANO DE POS GRADUACAO, 22., 2023, Sao Jose dos Campos, SP. Ciencias basicas e o desenvolvimento sustentavel: a interface dos saberes para a sociedade . Sao Jose dos Campos: UNIVAP, 2022.| Biblioteca(s): Biblioteca Rui Tendinha. |
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| 16. | | SILVA, F. G. da; SILVA, J. A. da; MILHEIROS, I. S.; RAIMUNDO, E. G.; CONCEIÇÃO, A. O. da; SENRA, J. F. de B.; ESPOSTI, M. D. Seleção de genótipos promissores de café conilon oriundos da cultivar ?ES8152?. In: ENCONTRO LATINO AMERICANO DE INICIACAO CIENTIFICA, 26.; ENCONTRO LATINO AMERICANO DE POS GRADUACAO, 22., 2023, Sao Jose dos Campos, SP. Ciencias basicas e o desenvolvimento sustentavel: a interface dos saberes para a sociedade . Sao Jose dos Campos: UNIVAP, 2022.| Biblioteca(s): Biblioteca Rui Tendinha. |
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| 17. | | CONCEICAO, A. O. da; SENRA, J. F. de B.; ESPOSTI, M. D.; SILVA, J. A. da; ZACARIAS, A. J.; SILVA, F. G. da; MILHEIROS, I. S. Selecao de genotipos promissores de cafe conilon em sistema agroflorestal com base no indice de area foliar. In: ENCONTRO LATINO AMERICANO DE INICIACAO CIENTIFICA, 26.; ENCONTRO LATINO AMERICANO DE POS GRADUACAO, 22., 2023, Sao Jose dos Campos, SP. Ciencias basicas e o desenvolvimento sustentavel: a interface dos saberes para a sociedade . Sao Jose dos Campos: UNIVAP, 2022.| Biblioteca(s): Biblioteca Rui Tendinha. |
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| 18. | | SENRA, J. F. de B.; SILVA, J. A. da; FERREIRA, A.; ESPOSTI, M. D.; SILVA, U. R.; MILHEIROS, I. S.; ZACARIAS, A. J. Seleção de modelos não lineares e o estudo do crescimento dos frutos de café conilon. Research, Society and Development, v. 11, n. 4, e21511427093, 2022.| Biblioteca(s): Biblioteca Rui Tendinha. |
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| 19. | | GADELHA, R. S. S.; FERNANDES, S. G.; CARVALHO, S. M. P. de; SILVA, J. A. da C. e; COSTA, R. A. da; OLIVEIRA, L. A. A. de; SARMENTO, W. da R. M. A cultura do abacaxi: perspectivas, tecnologias e viabilidade. Niterói: PESAGRO-RIO, 1996. 36 27 p. (PESAGRO-Rio. Documentos, 36).| Biblioteca(s): Biblioteca Rui Tendinha. |
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| 20. | | MALDONADO, J. F. M.; SILVA, J. A. da C. e; FERNANDES, S. G.; CARVALHO, S. M. P. de; COSTA, R. A. da; OLIVEIRA, L. A. A. de; SARMENTO, W. da R. M.; CUNHA, H.; CARVALHO, A. C. P. P. de A cultura da banana : perspectivas, tecnologias e viabilidade. Niterói-RJ : PESAGRO-RIO, 1998. 44p. (PESAGRO-RIO. Documentos, 45).| Biblioteca(s): Biblioteca Rui Tendinha. |
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Biblioteca(s): |
Biblioteca Rui Tendinha. |
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Data corrente: |
13/01/2015 |
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Data da última atualização: |
13/01/2015 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
MARRACCINI, P.; VINECKY, F.; ALVES, G. S. C.; RAMOS, H. J. O.; ELBELT, S.; VIEIRA, N. G.; CARNEIRO, F. A.; SUJII, P. S.; ALEKCEVETCH, J. C.; SILVA, V. A.; DaMATTA, F. M.; FERRÃO, M. A. G.; LEROY, T.; POT, D.; VIEIRA, L. G. E.; SILVA, F. R. da; ANDRADE, A. C. |
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Afiliação: |
Maria Amélia Gava Ferrão, Incaper/Embrapa Café. |
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Título: |
Differentially expressed genes and proteins upon drought acclimation in tolerant and sensitive genotypes of Coffea canephora. |
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Ano de publicação: |
2012 |
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Fonte/Imprenta: |
Journal of Experimental Botany, Oxford v. 63, n. 11, p. 4191-4212, 2012. |
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Idioma: |
Inglês |
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Conteúdo: |
The aim of this study was to investigate the molecular mechanisms underlying drought acclimation in coffee plants by the identification of candidate genes (CGs) using different approaches. The first approach used the data generated during the Brazilian Coffee expressed sequence tag (EST) project to select 13 CGs by an in silico analysis (electronic northern). The second approach was based on screening macroarrays spotted with plasmid DNA (coffee ESTs) with separate hybridizations using leaf cDNA probes from drought-tolerant and susceptible clones of Coffea canephora var. Conilon, grown under different water regimes. This allowed the isolation of seven additional CGs. The third approach used two-dimensional gel electrophoresis to identify proteins displaying differential accumulation in leaves of drought-tolerant and susceptible clones of C. canephora. Six of them were characterized by MALDI-TOF-MS/MS (matrix-assisted laser desorption-time of flight-tandem mass spectrometry) and the corresponding proteins were identified. Finally, additional CGs were selected from the literature, and quantitative real-time polymerase chain reaction (qPCR) was performed to analyse the expression of all identified CGs. Altogether, >40 genes presenting differential gene expression during drought acclimation were identified, some of them showing different expression profiles between drought-tolerant and susceptible clones. Based on the obtained results, it can be concluded that factors involved a complex network of responses probably involving the abscisic signalling pathway and nitric oxide are major molecular determinants that might explain the better efficiency in controlling stomata closure and transpiration displayed by drought-tolerant clones of C. canephora. MenosThe aim of this study was to investigate the molecular mechanisms underlying drought acclimation in coffee plants by the identification of candidate genes (CGs) using different approaches. The first approach used the data generated during the Brazilian Coffee expressed sequence tag (EST) project to select 13 CGs by an in silico analysis (electronic northern). The second approach was based on screening macroarrays spotted with plasmid DNA (coffee ESTs) with separate hybridizations using leaf cDNA probes from drought-tolerant and susceptible clones of Coffea canephora var. Conilon, grown under different water regimes. This allowed the isolation of seven additional CGs. The third approach used two-dimensional gel electrophoresis to identify proteins displaying differential accumulation in leaves of drought-tolerant and susceptible clones of C. canephora. Six of them were characterized by MALDI-TOF-MS/MS (matrix-assisted laser desorption-time of flight-tandem mass spectrometry) and the corresponding proteins were identified. Finally, additional CGs were selected from the literature, and quantitative real-time polymerase chain reaction (qPCR) was performed to analyse the expression of all identified CGs. Altogether, >40 genes presenting differential gene expression during drought acclimation were identified, some of them showing different expression profiles between drought-tolerant and susceptible clones. Based on the obtained results, it can be concluded that factors involved a... Mostrar Tudo |
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Palavras-Chave: |
Candidate gene; Coffea canephora; Differential expression; Drought acclimation; Genética; Proteomic; Proteômica; Real time PCR. |
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Thesagro: |
Café; Coffea canephora; Genetics. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 03034naa a2200445 a 4500
001 1004907
005 2015-01-13
008 2012 bl uuuu u00u1 u #d
100 1 $aMARRACCINI, P.
245 $aDifferentially expressed genes and proteins upon drought acclimation in tolerant and sensitive genotypes of Coffea canephora.$h[electronic resource]
260 $c2012
520 $aThe aim of this study was to investigate the molecular mechanisms underlying drought acclimation in coffee plants by the identification of candidate genes (CGs) using different approaches. The first approach used the data generated during the Brazilian Coffee expressed sequence tag (EST) project to select 13 CGs by an in silico analysis (electronic northern). The second approach was based on screening macroarrays spotted with plasmid DNA (coffee ESTs) with separate hybridizations using leaf cDNA probes from drought-tolerant and susceptible clones of Coffea canephora var. Conilon, grown under different water regimes. This allowed the isolation of seven additional CGs. The third approach used two-dimensional gel electrophoresis to identify proteins displaying differential accumulation in leaves of drought-tolerant and susceptible clones of C. canephora. Six of them were characterized by MALDI-TOF-MS/MS (matrix-assisted laser desorption-time of flight-tandem mass spectrometry) and the corresponding proteins were identified. Finally, additional CGs were selected from the literature, and quantitative real-time polymerase chain reaction (qPCR) was performed to analyse the expression of all identified CGs. Altogether, >40 genes presenting differential gene expression during drought acclimation were identified, some of them showing different expression profiles between drought-tolerant and susceptible clones. Based on the obtained results, it can be concluded that factors involved a complex network of responses probably involving the abscisic signalling pathway and nitric oxide are major molecular determinants that might explain the better efficiency in controlling stomata closure and transpiration displayed by drought-tolerant clones of C. canephora.
650 $aCafé
650 $aCoffea canephora
650 $aGenetics
653 $aCandidate gene
653 $aCoffea canephora
653 $aDifferential expression
653 $aDrought acclimation
653 $aGenética
653 $aProteomic
653 $aProteômica
653 $aReal time PCR
700 1 $aVINECKY, F.
700 1 $aALVES, G. S. C.
700 1 $aRAMOS, H. J. O.
700 1 $aELBELT, S.
700 1 $aVIEIRA, N. G.
700 1 $aCARNEIRO, F. A.
700 1 $aSUJII, P. S.
700 1 $aALEKCEVETCH, J. C.
700 1 $aSILVA, V. A.
700 1 $aDaMATTA, F. M.
700 1 $aFERRÃO, M. A. G.
700 1 $aLEROY, T.
700 1 $aPOT, D.
700 1 $aVIEIRA, L. G. E.
700 1 $aSILVA, F. R. da
700 1 $aANDRADE, A. C.
773 $tJournal of Experimental Botany, Oxford$gv. 63, n. 11, p. 4191-4212, 2012.
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