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 | Acesso ao texto completo restrito à biblioteca da Biblioteca Rui Tendinha. Para informações adicionais entre em contato com biblioteca@incaper.es.gov.br. |
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Registro Completo |
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Biblioteca(s): |
Biblioteca Rui Tendinha. |
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Data corrente: |
06/05/2016 |
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Data da última atualização: |
28/09/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SANTOS, C.; VENTURA, J. A.; LIMA, N. |
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Afiliação: |
Cledir Santos, CIBAMA/BIOREN-UFRO; Jose Aires Ventura, Incaper; Nelson Lima, Universidade do Minho. |
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Título: |
New insights for diagnosis of pineapple fusariosis by MALDI-TOF MS technique. |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
Current Microbiology, p. 1-8, apr. 2016. |
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Idioma: |
Português |
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Conteúdo: |
Fusarium is one of the most economically important fungal genus, since it includes many pathogenic species which cause a wide range of plant diseases. Morphological or molecular biology identification of Fusarium species is a limiting step in the fast diagnosis and treatment of plant disease caused by these fungi. Mass spectrometry by matrix-assisted laser/desorption ionisation-time-of-flight (MALDI-TOF)-based fingerprinting approach was applied to the fungal growth monitoring and direct detection of strain Fusarium guttiforme E-480 inoculated in both pineapple cultivars Pérola and Imperial side shoots, that are susceptible and resistant, respectively, to this fungal strain. MALDI-TOF MS technique was capable to detect fungal molecular mass peaks in the susceptible pineapple stem side shoot tissue. It is assumed that these molecular masses are mainly constituted by ribosomal proteins. MALDI-TOF-based fingerprinting approach has herein been demonstrated to be sensitive and accurate for the direct detection of F. guttiforme E-480 molecular masses on both susceptible and resistant pineapple side stem free of any pre-treatment. According to the results obtained, the changing on molecular mass peaks of infected susceptible pineapple tissue together with the possibility of fungal molecular masses analysis into this pineapple tissue can be a good indication for an early diagnosis by MALDI-TOF MS of pineapple fusariosis. |
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Thesaurus NAL: |
Fusarium; Pineapple. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01905naa a2200169 a 4500
001 1010791
005 2017-09-28
008 2016 bl uuuu u00u1 u #d
100 1 $aSANTOS, C.
245 $aNew insights for diagnosis of pineapple fusariosis by MALDI-TOF MS technique.$h[electronic resource]
260 $c2016
520 $aFusarium is one of the most economically important fungal genus, since it includes many pathogenic species which cause a wide range of plant diseases. Morphological or molecular biology identification of Fusarium species is a limiting step in the fast diagnosis and treatment of plant disease caused by these fungi. Mass spectrometry by matrix-assisted laser/desorption ionisation-time-of-flight (MALDI-TOF)-based fingerprinting approach was applied to the fungal growth monitoring and direct detection of strain Fusarium guttiforme E-480 inoculated in both pineapple cultivars Pérola and Imperial side shoots, that are susceptible and resistant, respectively, to this fungal strain. MALDI-TOF MS technique was capable to detect fungal molecular mass peaks in the susceptible pineapple stem side shoot tissue. It is assumed that these molecular masses are mainly constituted by ribosomal proteins. MALDI-TOF-based fingerprinting approach has herein been demonstrated to be sensitive and accurate for the direct detection of F. guttiforme E-480 molecular masses on both susceptible and resistant pineapple side stem free of any pre-treatment. According to the results obtained, the changing on molecular mass peaks of infected susceptible pineapple tissue together with the possibility of fungal molecular masses analysis into this pineapple tissue can be a good indication for an early diagnosis by MALDI-TOF MS of pineapple fusariosis.
650 $aFusarium
650 $aPineapple
700 1 $aVENTURA, J. A.
700 1 $aLIMA, N.
773 $tCurrent Microbiology, p. 1-8, apr. 2016.
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Registro original: |
Biblioteca Rui Tendinha (BRT) |
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| Acesso ao texto completo restrito à biblioteca da Biblioteca Rui Tendinha. Para informações adicionais entre em contato com biblioteca@incaper.es.gov.br. |
|
Registro Completo |
|
Biblioteca(s): |
Biblioteca Rui Tendinha. |
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Data corrente: |
03/02/2015 |
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Data da última atualização: |
01/09/2015 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 2 |
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Autoria: |
COSTA, H. B. da.; DELBONI, S. G.; FORTUNATO, F. da S.; VENTURA, J. A. |
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Afiliação: |
Jose Aires Ventura, Incaper. |
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Título: |
Proteolytic activity in pineapple (Ananas comosus var. comosus) stumps of cvs. Vitória, Smooth Cayenne and Pérola. |
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Ano de publicação: |
2009 |
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Fonte/Imprenta: |
Acta Horticulturae, v. 822, p. 239-244, 2009. |
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Idioma: |
Inglês |
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Conteúdo: |
Pineapple is one of the most important tropical fruits, not only due to its quality as fresh and processed product, but also as a source of subproducts such as bromelain, a proteolytic enzyme mixture with large applications in pharmaceutical and food industries. Looking for solutions for disease problems, Brazilian research institutions have developed new pineapple cultivars such as the ?Vitória?, released for farmers? use in the State of Espirito Santo in 2006. ?Vitória? plants are resistant to fusariosis, the main pineapple disease in Brazil, and show agronomic characteristics similar or even superior when compared to the traditional cvs. ?Pérola? and ?Smooth Cayenne?. In this work the total protein contents and the proteolytic and specific activities of proteolytic enzymes in pineapple stems of cvs. ?Vitória?, ?Pérola? and ?Smooth Cayenne? were determined. Results indicate that the new cv. ?Vitória? presented protein and enzymatic values in stem tissues similar to those found in the ?Smooth Cayenne? cultivar. |
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Palavras-Chave: |
Abacaxi; Fusariose. |
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Thesaurus NAL: |
Genotypes; Pineapple. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01644naa a2200205 a 4500
001 1005348
005 2015-09-01
008 2009 bl uuuu u00u1 u #d
100 1 $aCOSTA, H. B. da.
245 $aProteolytic activity in pineapple (Ananas comosus var. comosus) stumps of cvs. Vitória, Smooth Cayenne and Pérola.$h[electronic resource]
260 $c2009
520 $aPineapple is one of the most important tropical fruits, not only due to its quality as fresh and processed product, but also as a source of subproducts such as bromelain, a proteolytic enzyme mixture with large applications in pharmaceutical and food industries. Looking for solutions for disease problems, Brazilian research institutions have developed new pineapple cultivars such as the ?Vitória?, released for farmers? use in the State of Espirito Santo in 2006. ?Vitória? plants are resistant to fusariosis, the main pineapple disease in Brazil, and show agronomic characteristics similar or even superior when compared to the traditional cvs. ?Pérola? and ?Smooth Cayenne?. In this work the total protein contents and the proteolytic and specific activities of proteolytic enzymes in pineapple stems of cvs. ?Vitória?, ?Pérola? and ?Smooth Cayenne? were determined. Results indicate that the new cv. ?Vitória? presented protein and enzymatic values in stem tissues similar to those found in the ?Smooth Cayenne? cultivar.
650 $aGenotypes
650 $aPineapple
653 $aAbacaxi
653 $aFusariose
700 1 $aDELBONI, S. G.
700 1 $aFORTUNATO, F. da S.
700 1 $aVENTURA, J. A.
773 $tActa Horticulturae$gv. 822, p. 239-244, 2009.
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