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http://biblioteca.incaper.es.gov.br/digital/handle/123456789/3593| Título: | Bioinformatics approach to the study of the molecular behaviour of mealybug wilt of pineapple. |
| Autor(es): | PERON, F. N.  CALLONI, R. VENTURA, J. A. FERNANDES, P. M. B. UFES; UFGRS; Jose Aires Ventura, Incaper; UFES. |
| Palavras-chave: | Abacaxi |
| Data do documento: | 24-Jun-2019 |
| Editor: | Acta Horticulturae, 1239, p. 177-184, 2019. |
| Descrição: | Mealybug wilt of pineapple (MWP) is a disease caused by the Pineapple mealybug wilt-associated virus (PMWaV) complex transmitted by Dysmicoccus brevipes and D. neobrevipes. MWP symptoms are characterized by root dessication, leaf wilting and consequent failure to produce a fruit. The molecular mechanisms involved in the pineapple-PMWaV interaction for MWP symptomatology are still unclear. In this work, mRNAs of asymptomatic and symptomatic pineapple plants were evaluated using Illumina RNA sequencing technology. From a total of 79 million reads per sample, 16,097 genes were identified using STAR aligner and HTseq for paired-end files. Differentially expressed genes (DEGs) between the evaluated groups were estimated using DESeq2 and edgeR, with an FDR cutoff of ≤0.05. A total of 207 DEGs were detected, with 61 upregulated and 146 downregulated in symptomatic plants infected by PMWaV-2. The methodologies improved by the assays presented in this article and the detected DEGs can substantiate further researches with pineapple and the MWP disease. |
| URI: | http://biblioteca.incaper.es.gov.br/digital/handle/123456789/3593 |
| Aparece nas coleções: | Memória Técnica do Incaper |
Arquivos associados a este item:
| Arquivo | Descrição | Tamanho | Formato | |
|---|---|---|---|---|
| Bioinformatics-approach-to-the-molecular-study-Mealybug-wilt-pineapple-2019.pdf | 465,2 kB | Adobe PDF | Visualizar/Abrir |
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