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http://biblioteca.incaper.es.gov.br/digital/handle/123456789/4040| Título: | A multiplex RT‑PCR method to detect papaya meleira virus complex in adult pre‑flowering plants. |
| Autor(es): | MAURASTONI, M.  SÁ-ANTUNES, T. F. OLIVEIRA, S. SANTOS, A. M. C. VENTURA, J. A. FERNANDES, P. M. B. Marlonni Maurastoni, UFES; Tathiana F. Sá?Antunes, UFES; Scarlett A. Oliveira, UFES; Alexandre M. C. Santos, UFES; Jose Aires Ventura, Incaper; Patricia M. B. Fernandes, UFES. |
| Palavras-chave: | Doença das Planta Papaya meleira virus (PMeV) Multiplex |
| Data do documento: | 5-Jul-2020 |
| Editor: | Arch Virol, v. 165, p. 1211 1214, july 2020. |
| Descrição: | Papaya sticky disease (PSD), which can destroy orchards, was first attributed to papaya meleira virus (PMeV). However, the discovery of papaya meleira virus 2 (PMeV2) associated with PSD plants impose the need to detect this viral complex. We developed a multiplex RT-PCR (mPCR) technique capable of detecting two viruses in a single assay from pre-flowering plant samples, which is a useful tool for early diagnosis of PSD. We also determined the limit of detection (LOD) using asymmetric plasmid dilutions of both PMeV and PMeV2, which revealed that a higher titer of one virus prevents detection of the other. Thus, this technique is an alternative method for detecting PMeV and PMeV2 in a single reaction. |
| URI: | http://biblioteca.incaper.es.gov.br/digital/handle/123456789/4040 |
| Aparece nas coleções: | Memória Técnica do Incaper |
Arquivos associados a este item:
| Arquivo | Descrição | Tamanho | Formato | |
|---|---|---|---|---|
| multiplex-meleira-ventura.pdf | 558,06 kB | Adobe PDF | Visualizar/Abrir |
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