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http://biblioteca.incaper.es.gov.br/digital/handle/item/162| Título: | Molecular diagnosis of Papaya meleira virus (PMeV) from leaf samples of Carica papaya L. using conventional and real-time RT-PCR. |
| Autor(es): | ABREU, P. M. V.  PICCIN, J. G. RODRIGUES, S. P. VENTURA. J. A. FERNANDES, P. M. B. Paolla M.V. Abreu, UFES; João G. Piccin, UFES; Silas P. Rodrigues, UFES; Jose Aires Ventura, Incaper; Patricia M.B. Fernandes, UFES. |
| Palavras-chave: | Papaya meleira virus Papaya Resistance induction Phytopathology DsRNA Real-time RT-PCR |
| Data do documento: | 8-Jan-2014 |
| Editor: | Crop Protection, vol. 36, p. 7-10, 2012. |
| Descrição: | Papaya meleira virus (PMeV) is the causal agent of papaya sticky disease. This study describes two methods for molecular diagnosis of PMeV using conventional and real-time PCR. These methods were shown to be more efficient than current methods of viral detection using extraction of PMeV dsRNA and observation of symptoms in the field. The methods described here were used to evaluate the effect of inoculation of papaya plants with purified PMeV dsRNA on the progress of PMeV infection. A single inoculation with PMeV dsRNA was observed to delay the progress of the virus infection by several weeks. The possibility of vertical transmission of PMeV was also investigated. No evidence was found for PMeV transmission through seeds collected from diseased fruit. The implications of these results for the epidemiology of PMeV and the management of papaya sticky disease are discussed. |
| URI: | http://ac.els-cdn.com/S0166093411004885/1-s2.0-S0166093411004885-main.pdf?_tid=597935f2-7857-11e3-a060-00000aacb35e&acdnat=1389180411_31ada3a608c8d17c0a26fd345cb062ae http://localhost:8080/digital/handle/item/162 http://biblioteca.incaper.es.gov.br/digital/handle/item/162 |
| Aparece nas coleções: | Memória Técnica do Incaper |
Arquivos associados a este item:
| Arquivo | Descrição | Tamanho | Formato | |
|---|---|---|---|---|
| JVirolMethods-2012.PDF | 404,56 kB | Adobe PDF |  Visualizar/Abrir |
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